Copper transporter Ctr1 contributes to enhancement of the sensitivity of cisplatin in esophageal squamous cell carcinoma
Increasing evidence has demonstrated that Ctr1 plays a crucial role in the regulation of cisplatin uptake in a variety of tumors. The purpose of this study was to investigate its role in mediating cisplatin sensitivity in ESCC cells. Immunohistochemistry (IHC), In situ hybridization (ISH) and semi-q...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | Translational Oncology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1936523323000116 |
| _version_ | 1797936419082076160 |
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| author | Xin Wang Qianqian Lou Tianli Fan Qing Zhang Xiangxiang Yang Hongtao Liu Ruitai Fan |
| author_facet | Xin Wang Qianqian Lou Tianli Fan Qing Zhang Xiangxiang Yang Hongtao Liu Ruitai Fan |
| author_sort | Xin Wang |
| collection | DOAJ |
| description | Increasing evidence has demonstrated that Ctr1 plays a crucial role in the regulation of cisplatin uptake in a variety of tumors. The purpose of this study was to investigate its role in mediating cisplatin sensitivity in ESCC cells. Immunohistochemistry (IHC), In situ hybridization (ISH) and semi-quantitative RT-PCR were used to detect Ctr1 expressions in ESCC tissues. qRT-PCR and Western blot was performed to investigate the levels of Ctr1 mRNA and protein in ESCC cells. CCK-8, Flow cytometry and Transwell chamber assay were carried out to examine cell proliferation, apoptosis, migration and invasion abilities in ESCC cells. We found that ESCC tissues and cells had higher Ctr1 level than normal tissues and Het-1A cell. Ctr1 expression was correlated with histological grade, invasion depth, TNM staging and lymph node metastasis in ESCC patients. Ctr1 depletion reduced the suppressive role of proliferation, migration and invasion as well as the inductive role of cell apoptosis and Caspase-3 activity evoked by cisplatin, whereas Ctr1 upregulation combined with cisplatin exerted the synergistic role in regulation of proliferation, apoptosis, Caspase-3 activity, migration and invasion in ESCC. In conclusion, Ctr1 is implicated in ESCC development and progression and its expression may be a novel predictor for assessment of cisplatin sensitivity in ESCC. |
| first_indexed | 2024-04-10T18:30:30Z |
| format | Article |
| id | doaj.art-fabf70e474f24b8ca50d67af5c66a524 |
| institution | Directory Open Access Journal |
| issn | 1936-5233 |
| language | English |
| last_indexed | 2024-04-10T18:30:30Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Translational Oncology |
| spelling | doaj.art-fabf70e474f24b8ca50d67af5c66a5242023-02-02T04:48:11ZengElsevierTranslational Oncology1936-52332023-03-0129101626Copper transporter Ctr1 contributes to enhancement of the sensitivity of cisplatin in esophageal squamous cell carcinomaXin Wang0Qianqian Lou1Tianli Fan2Qing Zhang3Xiangxiang Yang4Hongtao Liu5Ruitai Fan6Department of Radiotherapy, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450052, ChinaSchool of Life Sciences, Zhengzhou University, Zhengzhou, Henan, 450001, ChinaDepartment of Pharmacology, School of Basic Medicine, Zhengzhou University, 100 Kexue Road, Zhengzhou, Henan, 450001, ChinaTranslational Medicine Research Center, Zhengzhou People's Hospital, Zhengzhou, Henan, 450003, ChinaDepartment of Radiotherapy, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450052, ChinaSchool of Life Sciences, Zhengzhou University, Zhengzhou, Henan, 450001, China; Translational Medicine Research Center, Zhengzhou People's Hospital, Zhengzhou, Henan, 450003, China; Corresponding author at: College of Life Sciences, Zhengzhou University, 100 Kexue Road, Zhengzhou, 450001, China.Department of Radiotherapy, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, 450052, China; Corresponding author at: Department of Radiotherapy, the First Affiliated Hospital of Zhengzhou University, No. 1, Jianshe East Road, Zhengzhou, Henan, 450052, China.Increasing evidence has demonstrated that Ctr1 plays a crucial role in the regulation of cisplatin uptake in a variety of tumors. The purpose of this study was to investigate its role in mediating cisplatin sensitivity in ESCC cells. Immunohistochemistry (IHC), In situ hybridization (ISH) and semi-quantitative RT-PCR were used to detect Ctr1 expressions in ESCC tissues. qRT-PCR and Western blot was performed to investigate the levels of Ctr1 mRNA and protein in ESCC cells. CCK-8, Flow cytometry and Transwell chamber assay were carried out to examine cell proliferation, apoptosis, migration and invasion abilities in ESCC cells. We found that ESCC tissues and cells had higher Ctr1 level than normal tissues and Het-1A cell. Ctr1 expression was correlated with histological grade, invasion depth, TNM staging and lymph node metastasis in ESCC patients. Ctr1 depletion reduced the suppressive role of proliferation, migration and invasion as well as the inductive role of cell apoptosis and Caspase-3 activity evoked by cisplatin, whereas Ctr1 upregulation combined with cisplatin exerted the synergistic role in regulation of proliferation, apoptosis, Caspase-3 activity, migration and invasion in ESCC. In conclusion, Ctr1 is implicated in ESCC development and progression and its expression may be a novel predictor for assessment of cisplatin sensitivity in ESCC.http://www.sciencedirect.com/science/article/pii/S1936523323000116Esophageal squamous cell carcinomaCopper transporter 1CisplatinAntitumor efficacy |
| spellingShingle | Xin Wang Qianqian Lou Tianli Fan Qing Zhang Xiangxiang Yang Hongtao Liu Ruitai Fan Copper transporter Ctr1 contributes to enhancement of the sensitivity of cisplatin in esophageal squamous cell carcinoma Translational Oncology Esophageal squamous cell carcinoma Copper transporter 1 Cisplatin Antitumor efficacy |
| title | Copper transporter Ctr1 contributes to enhancement of the sensitivity of cisplatin in esophageal squamous cell carcinoma |
| title_full | Copper transporter Ctr1 contributes to enhancement of the sensitivity of cisplatin in esophageal squamous cell carcinoma |
| title_fullStr | Copper transporter Ctr1 contributes to enhancement of the sensitivity of cisplatin in esophageal squamous cell carcinoma |
| title_full_unstemmed | Copper transporter Ctr1 contributes to enhancement of the sensitivity of cisplatin in esophageal squamous cell carcinoma |
| title_short | Copper transporter Ctr1 contributes to enhancement of the sensitivity of cisplatin in esophageal squamous cell carcinoma |
| title_sort | copper transporter ctr1 contributes to enhancement of the sensitivity of cisplatin in esophageal squamous cell carcinoma |
| topic | Esophageal squamous cell carcinoma Copper transporter 1 Cisplatin Antitumor efficacy |
| url | http://www.sciencedirect.com/science/article/pii/S1936523323000116 |
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