A new apparatus for standardized rat kidney biopsy.
Survival biopsies are frequently applied in rat kidney disease models, but several drawbacks such as surgical kidney trauma, bleeding risk and variable loss of kidney tissue are still unsolved. Therefore, we developed an easy-to-use core biopsy instrument and evaluated whether two consecutive kidney...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4266664?pdf=render |
| _version_ | 1828393374793596928 |
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| author | Holger Schirutschke Lars Gladrow Christian Norkus Simon Paul Parmentier Bernd Hohenstein Christian P M Hugo |
| author_facet | Holger Schirutschke Lars Gladrow Christian Norkus Simon Paul Parmentier Bernd Hohenstein Christian P M Hugo |
| author_sort | Holger Schirutschke |
| collection | DOAJ |
| description | Survival biopsies are frequently applied in rat kidney disease models, but several drawbacks such as surgical kidney trauma, bleeding risk and variable loss of kidney tissue are still unsolved. Therefore, we developed an easy-to-use core biopsy instrument and evaluated whether two consecutive kidney biopsies within the same kidney can be carried out in a standardized manner. On day 0, 18 Lewis rats underwent a right nephrectomy and 9 of these rats a subsequent first biopsy of the left kidney (Bx group). 9 control rats had a sham biopsy of the left kidney (Ctrl group). On day 7, a second kidney biopsy/sham biopsy was performed. On day 42, all animals were sacrificed and their kidneys were removed for histology. Biopsy cylinders contained 57±28 glomeruli per transversal section, representing an adequate sample size. PAS staining showed that the biopsy depth was limited to the renal cortex whereas surgical tissue damage was limited to the area immediately adjacent to the taken biopsy cylinder. On day 42, the reduction of functional renal mass after two biopsies was only 5.2% and no differences of body weight, blood pressure, proteinuria, serum creatinine, glomerulosclerosis, interstitial fibrosis or number of ED-1 positive macrophages were found between both groups. In summary, our apparatus offers a safe method to perform repetitive kidney biopsies with minimal trauma and sufficient sample size and quality even in experimental disease models restricted to one single kidney. |
| first_indexed | 2024-12-10T07:38:12Z |
| format | Article |
| id | doaj.art-fac3c8cb65554dd0a6ba95869bd8a3b2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T07:38:12Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS ONE |
| spelling | doaj.art-fac3c8cb65554dd0a6ba95869bd8a3b22022-12-22T01:57:23ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01912e11536810.1371/journal.pone.0115368A new apparatus for standardized rat kidney biopsy.Holger SchirutschkeLars GladrowChristian NorkusSimon Paul ParmentierBernd HohensteinChristian P M HugoSurvival biopsies are frequently applied in rat kidney disease models, but several drawbacks such as surgical kidney trauma, bleeding risk and variable loss of kidney tissue are still unsolved. Therefore, we developed an easy-to-use core biopsy instrument and evaluated whether two consecutive kidney biopsies within the same kidney can be carried out in a standardized manner. On day 0, 18 Lewis rats underwent a right nephrectomy and 9 of these rats a subsequent first biopsy of the left kidney (Bx group). 9 control rats had a sham biopsy of the left kidney (Ctrl group). On day 7, a second kidney biopsy/sham biopsy was performed. On day 42, all animals were sacrificed and their kidneys were removed for histology. Biopsy cylinders contained 57±28 glomeruli per transversal section, representing an adequate sample size. PAS staining showed that the biopsy depth was limited to the renal cortex whereas surgical tissue damage was limited to the area immediately adjacent to the taken biopsy cylinder. On day 42, the reduction of functional renal mass after two biopsies was only 5.2% and no differences of body weight, blood pressure, proteinuria, serum creatinine, glomerulosclerosis, interstitial fibrosis or number of ED-1 positive macrophages were found between both groups. In summary, our apparatus offers a safe method to perform repetitive kidney biopsies with minimal trauma and sufficient sample size and quality even in experimental disease models restricted to one single kidney.http://europepmc.org/articles/PMC4266664?pdf=render |
| spellingShingle | Holger Schirutschke Lars Gladrow Christian Norkus Simon Paul Parmentier Bernd Hohenstein Christian P M Hugo A new apparatus for standardized rat kidney biopsy. PLoS ONE |
| title | A new apparatus for standardized rat kidney biopsy. |
| title_full | A new apparatus for standardized rat kidney biopsy. |
| title_fullStr | A new apparatus for standardized rat kidney biopsy. |
| title_full_unstemmed | A new apparatus for standardized rat kidney biopsy. |
| title_short | A new apparatus for standardized rat kidney biopsy. |
| title_sort | new apparatus for standardized rat kidney biopsy |
| url | http://europepmc.org/articles/PMC4266664?pdf=render |
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