Ergosterol and Its Metabolites Induce Ligninolytic Activity in the Lignin-Degrading Fungus <i>Phanerochaete sordida</i> YK-624
White-rot fungi are the most important group of lignin biodegraders. <i>Phanerochaete sordida</i> YK-624 has higher ligninolytic activity than that of model white-rot fungi. However, the underlying mechanism responsible for lignin degradation by white-rot fungi remains unknown, and the i...
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MDPI AG
2023-09-01
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| Series: | Journal of Fungi |
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| author | Jianqiao Wang Ru Yin Yuki Hashizume Yasushi Todoroki Toshio Mori Hirokazu Kawagishi Hirofumi Hirai |
| author_facet | Jianqiao Wang Ru Yin Yuki Hashizume Yasushi Todoroki Toshio Mori Hirokazu Kawagishi Hirofumi Hirai |
| author_sort | Jianqiao Wang |
| collection | DOAJ |
| description | White-rot fungi are the most important group of lignin biodegraders. <i>Phanerochaete sordida</i> YK-624 has higher ligninolytic activity than that of model white-rot fungi. However, the underlying mechanism responsible for lignin degradation by white-rot fungi remains unknown, and the induced compounds isolated from white-rot fungi for lignin degradation have never been studied. In the present study, we tried to screen ligninolytic-inducing compounds produced by <i>P. sordida</i> YK-624. After large-scale incubation of <i>P. sordida</i> YK-624, the culture and mycelium were separated by filtration. After the separation and purification, purified compounds were analyzed by high-resolution electrospray ionization mass spectrometry and nuclear magnetic resonance. The sterilized unbleached hardwood kraft pulp was used for the initial evaluation of ligninolytic activity. Ergosterol was isolated and identified and it induced the lignin-degrading activity of this fungus. Moreover, we investigated ergosterol metabolites from <i>P. sordida</i> YK-624, and the ergosterol metabolites ergosta-4,7,22-triene-3,6-dione and ergosta-4,6,8(14),22-tetraen-3-one were identified and then chemically synthesized. These compounds significantly improved the lignin-degrading activity of the fungus. This is the first report on the ligninolytic-inducing compounds produced by white-rot fungi. |
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| spelling | doaj.art-fac3fb7155584a009b7bfec21245f8d72023-11-19T11:29:34ZengMDPI AGJournal of Fungi2309-608X2023-09-019995110.3390/jof9090951Ergosterol and Its Metabolites Induce Ligninolytic Activity in the Lignin-Degrading Fungus <i>Phanerochaete sordida</i> YK-624Jianqiao Wang0Ru Yin1Yuki Hashizume2Yasushi Todoroki3Toshio Mori4Hirokazu Kawagishi5Hirofumi Hirai6Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, School of Environmental Science and Engineering, Guangzhou University, Guangzhou 510006, ChinaGraduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, JapanFaculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, JapanFaculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, JapanFaculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, JapanFaculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, JapanResearch Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, JapanWhite-rot fungi are the most important group of lignin biodegraders. <i>Phanerochaete sordida</i> YK-624 has higher ligninolytic activity than that of model white-rot fungi. However, the underlying mechanism responsible for lignin degradation by white-rot fungi remains unknown, and the induced compounds isolated from white-rot fungi for lignin degradation have never been studied. In the present study, we tried to screen ligninolytic-inducing compounds produced by <i>P. sordida</i> YK-624. After large-scale incubation of <i>P. sordida</i> YK-624, the culture and mycelium were separated by filtration. After the separation and purification, purified compounds were analyzed by high-resolution electrospray ionization mass spectrometry and nuclear magnetic resonance. The sterilized unbleached hardwood kraft pulp was used for the initial evaluation of ligninolytic activity. Ergosterol was isolated and identified and it induced the lignin-degrading activity of this fungus. Moreover, we investigated ergosterol metabolites from <i>P. sordida</i> YK-624, and the ergosterol metabolites ergosta-4,7,22-triene-3,6-dione and ergosta-4,6,8(14),22-tetraen-3-one were identified and then chemically synthesized. These compounds significantly improved the lignin-degrading activity of the fungus. This is the first report on the ligninolytic-inducing compounds produced by white-rot fungi.https://www.mdpi.com/2309-608X/9/9/951lignin degradationwhite-rot fungi<i>Phanerochaete sordida</i> YK-624inducing compounds |
| spellingShingle | Jianqiao Wang Ru Yin Yuki Hashizume Yasushi Todoroki Toshio Mori Hirokazu Kawagishi Hirofumi Hirai Ergosterol and Its Metabolites Induce Ligninolytic Activity in the Lignin-Degrading Fungus <i>Phanerochaete sordida</i> YK-624 Journal of Fungi lignin degradation white-rot fungi <i>Phanerochaete sordida</i> YK-624 inducing compounds |
| title | Ergosterol and Its Metabolites Induce Ligninolytic Activity in the Lignin-Degrading Fungus <i>Phanerochaete sordida</i> YK-624 |
| title_full | Ergosterol and Its Metabolites Induce Ligninolytic Activity in the Lignin-Degrading Fungus <i>Phanerochaete sordida</i> YK-624 |
| title_fullStr | Ergosterol and Its Metabolites Induce Ligninolytic Activity in the Lignin-Degrading Fungus <i>Phanerochaete sordida</i> YK-624 |
| title_full_unstemmed | Ergosterol and Its Metabolites Induce Ligninolytic Activity in the Lignin-Degrading Fungus <i>Phanerochaete sordida</i> YK-624 |
| title_short | Ergosterol and Its Metabolites Induce Ligninolytic Activity in the Lignin-Degrading Fungus <i>Phanerochaete sordida</i> YK-624 |
| title_sort | ergosterol and its metabolites induce ligninolytic activity in the lignin degrading fungus i phanerochaete sordida i yk 624 |
| topic | lignin degradation white-rot fungi <i>Phanerochaete sordida</i> YK-624 inducing compounds |
| url | https://www.mdpi.com/2309-608X/9/9/951 |
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