Development of a monoclonal antibody against bovine α-casein to evaluate functional status of mammary epithelial cells during mastitis
Mastitis is a widespread disease in dairy animals and causes huge economic losses around the globe. Mastitis affects the health of animals and also reduces the protein content in the milk. In lactating animals, the surface of mammary gland is coated and lined with mammary epithelial cells (MECs). Th...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Kafkas University, Faculty of Veterinary Medicine
2019-03-01
|
| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| Subjects: | |
| Online Access: | https://vetdergikafkas.org/pdf.php?id=2499 |
| _version_ | 1827922243669196800 |
|---|---|
| author | Wentao MA Yi WANG Fei GAO Mengxia NING Ahua LIU Yanyan LI Yang GAO Ping LU Dekun CHEN |
| author_facet | Wentao MA Yi WANG Fei GAO Mengxia NING Ahua LIU Yanyan LI Yang GAO Ping LU Dekun CHEN |
| author_sort | Wentao MA |
| collection | DOAJ |
| description | Mastitis is a widespread disease in dairy animals and causes huge economic losses around the globe. Mastitis affects the health of animals and also
reduces the protein content in the milk. In lactating animals, the surface of mammary gland is coated and lined with mammary epithelial cells (MECs).
The functional status of MECs can be evaluated by determining the expression of caseins. The total content of caseins can largely reflect the quality
of milk. In the previous literature, there is no general information about the milk quality and expressions of casein in mastitis. The current study was
designed to determine if there is any correlations between mastitis and α-casein expression in MECs. We prepared a hybridoma cell line that produces
antibody against bovine α-casein to evaluate the casein expression status in vitro on the MECs during lipopolysaccharide (LPS)-induced mastitis. The
results showed that the expression of α-casein has not changed significantly under the stimulation of LPS. Our study established a useful tool to
determine the expression profile of α-casein in bovine MECs. Furthermore, the result indicated that the expression level of α-casein remained stable
during mastitis. |
| first_indexed | 2024-03-13T04:38:54Z |
| format | Article |
| id | doaj.art-fac53a655bf34027abd2fdb1dcb429fd |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:38:54Z |
| publishDate | 2019-03-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-fac53a655bf34027abd2fdb1dcb429fd2023-06-19T06:34:21ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512019-03-0125444545010.9775/kvfd.2018.208972499Development of a monoclonal antibody against bovine α-casein to evaluate functional status of mammary epithelial cells during mastitisWentao MA0Yi WANG1Fei GAO2Mengxia NING3Ahua LIU4Yanyan LI5Yang GAO6Ping LU7Dekun CHENVeterinary Immunology Laboratory, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, Shaanxi Province, CHINAVeterinary Immunology Laboratory, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, Shaanxi Province, CHINAVeterinary Immunology Laboratory, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, Shaanxi Province, CHINAVeterinary Immunology Laboratory, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, Shaanxi Province, CHINAVeterinary Immunology Laboratory, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, Shaanxi Province, CHINAVeterinary Immunology Laboratory, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, Shaanxi Province, CHINAVeterinary Immunology Laboratory, College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, Shaanxi Province, CHINAChina Animal Health and Epidemiology Center, Qingdao 266032, Shandong Province, CHINAMastitis is a widespread disease in dairy animals and causes huge economic losses around the globe. Mastitis affects the health of animals and also reduces the protein content in the milk. In lactating animals, the surface of mammary gland is coated and lined with mammary epithelial cells (MECs). The functional status of MECs can be evaluated by determining the expression of caseins. The total content of caseins can largely reflect the quality of milk. In the previous literature, there is no general information about the milk quality and expressions of casein in mastitis. The current study was designed to determine if there is any correlations between mastitis and α-casein expression in MECs. We prepared a hybridoma cell line that produces antibody against bovine α-casein to evaluate the casein expression status in vitro on the MECs during lipopolysaccharide (LPS)-induced mastitis. The results showed that the expression of α-casein has not changed significantly under the stimulation of LPS. Our study established a useful tool to determine the expression profile of α-casein in bovine MECs. Furthermore, the result indicated that the expression level of α-casein remained stable during mastitis.https://vetdergikafkas.org/pdf.php?id=2499α-caseinmonoclonal antibodymammary epithelial cellsmastitis |
| spellingShingle | Wentao MA Yi WANG Fei GAO Mengxia NING Ahua LIU Yanyan LI Yang GAO Ping LU Dekun CHEN Development of a monoclonal antibody against bovine α-casein to evaluate functional status of mammary epithelial cells during mastitis Kafkas Universitesi Veteriner Fakültesi Dergisi α-casein monoclonal antibody mammary epithelial cells mastitis |
| title | Development of a monoclonal antibody against bovine α-casein to evaluate functional status of mammary epithelial cells during mastitis |
| title_full | Development of a monoclonal antibody against bovine α-casein to evaluate functional status of mammary epithelial cells during mastitis |
| title_fullStr | Development of a monoclonal antibody against bovine α-casein to evaluate functional status of mammary epithelial cells during mastitis |
| title_full_unstemmed | Development of a monoclonal antibody against bovine α-casein to evaluate functional status of mammary epithelial cells during mastitis |
| title_short | Development of a monoclonal antibody against bovine α-casein to evaluate functional status of mammary epithelial cells during mastitis |
| title_sort | development of a monoclonal antibody against bovine α casein to evaluate functional status of mammary epithelial cells during mastitis |
| topic | α-casein monoclonal antibody mammary epithelial cells mastitis |
| url | https://vetdergikafkas.org/pdf.php?id=2499 |
| work_keys_str_mv | AT wentaoma developmentofamonoclonalantibodyagainstbovineacaseintoevaluatefunctionalstatusofmammaryepithelialcellsduringmastitis AT yiwang developmentofamonoclonalantibodyagainstbovineacaseintoevaluatefunctionalstatusofmammaryepithelialcellsduringmastitis AT feigao developmentofamonoclonalantibodyagainstbovineacaseintoevaluatefunctionalstatusofmammaryepithelialcellsduringmastitis AT mengxianing developmentofamonoclonalantibodyagainstbovineacaseintoevaluatefunctionalstatusofmammaryepithelialcellsduringmastitis AT ahualiu developmentofamonoclonalantibodyagainstbovineacaseintoevaluatefunctionalstatusofmammaryepithelialcellsduringmastitis AT yanyanli developmentofamonoclonalantibodyagainstbovineacaseintoevaluatefunctionalstatusofmammaryepithelialcellsduringmastitis AT yanggao developmentofamonoclonalantibodyagainstbovineacaseintoevaluatefunctionalstatusofmammaryepithelialcellsduringmastitis AT pinglu developmentofamonoclonalantibodyagainstbovineacaseintoevaluatefunctionalstatusofmammaryepithelialcellsduringmastitis AT dekunchen developmentofamonoclonalantibodyagainstbovineacaseintoevaluatefunctionalstatusofmammaryepithelialcellsduringmastitis |