Overview of Gene Expression Dynamics during Human Oogenesis/Folliculogenesis
The oocyte transcriptome follows a tightly controlled dynamic that leads the oocyte to grow and mature. This succession of distinct transcriptional states determines embryonic development prior to embryonic genome activation. However, these oocyte maternal mRNA regulatory events have yet to be decod...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/1/33 |
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| author | Bastien Ducreux Lucile Ferreux Catherine Patrat Patricia Fauque |
| author_facet | Bastien Ducreux Lucile Ferreux Catherine Patrat Patricia Fauque |
| author_sort | Bastien Ducreux |
| collection | DOAJ |
| description | The oocyte transcriptome follows a tightly controlled dynamic that leads the oocyte to grow and mature. This succession of distinct transcriptional states determines embryonic development prior to embryonic genome activation. However, these oocyte maternal mRNA regulatory events have yet to be decoded in humans. We reanalyzed human single-oocyte RNA-seq datasets previously published in the literature to decrypt the transcriptomic reshuffles ensuring that the oocyte is fully competent. We applied trajectory analysis (pseudotime) and a meta-analysis and uncovered the fundamental transcriptomic requirements of the oocyte at any moment of oogenesis until reaching the metaphase II stage (MII). We identified a bunch of genes showing significant variation in expression from primordial-to-antral follicle oocyte development and characterized their temporal regulation and their biological relevance. We also revealed the selective regulation of specific transcripts during the germinal vesicle-to-MII transition. Transcripts associated with energy production and mitochondrial functions were extensively downregulated, while those associated with cytoplasmic translation, histone modification, meiotic processes, and RNA processes were conserved. From the genes identified in this study, some appeared as sensitive to environmental factors such as maternal age, polycystic ovary syndrome, cryoconservation, and in vitro maturation. In the future, the atlas of transcriptomic changes described in this study will enable more precise identification of the transcripts responsible for follicular growth and oocyte maturation failures. |
| first_indexed | 2024-03-08T15:06:57Z |
| format | Article |
| id | doaj.art-fac97900e37f4ed297ca2adffa764c31 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T15:06:57Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-fac97900e37f4ed297ca2adffa764c312024-01-10T14:57:54ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-12-012513310.3390/ijms25010033Overview of Gene Expression Dynamics during Human Oogenesis/FolliculogenesisBastien Ducreux0Lucile Ferreux1Catherine Patrat2Patricia Fauque3Université Bourgogne Franche-Comté-Equipe Génétique des Anomalies du Développement (GAD) INSERM UMR1231, 2 Rue Angélique Ducoudray, F-21000 Dijon, FranceFaculty of Medicine, Inserm 1016, Université de Paris Cité, F-75014 Paris, FranceFaculty of Medicine, Inserm 1016, Université de Paris Cité, F-75014 Paris, FranceUniversité Bourgogne Franche-Comté-Equipe Génétique des Anomalies du Développement (GAD) INSERM UMR1231, 2 Rue Angélique Ducoudray, F-21000 Dijon, FranceThe oocyte transcriptome follows a tightly controlled dynamic that leads the oocyte to grow and mature. This succession of distinct transcriptional states determines embryonic development prior to embryonic genome activation. However, these oocyte maternal mRNA regulatory events have yet to be decoded in humans. We reanalyzed human single-oocyte RNA-seq datasets previously published in the literature to decrypt the transcriptomic reshuffles ensuring that the oocyte is fully competent. We applied trajectory analysis (pseudotime) and a meta-analysis and uncovered the fundamental transcriptomic requirements of the oocyte at any moment of oogenesis until reaching the metaphase II stage (MII). We identified a bunch of genes showing significant variation in expression from primordial-to-antral follicle oocyte development and characterized their temporal regulation and their biological relevance. We also revealed the selective regulation of specific transcripts during the germinal vesicle-to-MII transition. Transcripts associated with energy production and mitochondrial functions were extensively downregulated, while those associated with cytoplasmic translation, histone modification, meiotic processes, and RNA processes were conserved. From the genes identified in this study, some appeared as sensitive to environmental factors such as maternal age, polycystic ovary syndrome, cryoconservation, and in vitro maturation. In the future, the atlas of transcriptomic changes described in this study will enable more precise identification of the transcripts responsible for follicular growth and oocyte maturation failures.https://www.mdpi.com/1422-0067/25/1/33folliculogenesismaturationoocyteRNA-seqtranscriptome |
| spellingShingle | Bastien Ducreux Lucile Ferreux Catherine Patrat Patricia Fauque Overview of Gene Expression Dynamics during Human Oogenesis/Folliculogenesis International Journal of Molecular Sciences folliculogenesis maturation oocyte RNA-seq transcriptome |
| title | Overview of Gene Expression Dynamics during Human Oogenesis/Folliculogenesis |
| title_full | Overview of Gene Expression Dynamics during Human Oogenesis/Folliculogenesis |
| title_fullStr | Overview of Gene Expression Dynamics during Human Oogenesis/Folliculogenesis |
| title_full_unstemmed | Overview of Gene Expression Dynamics during Human Oogenesis/Folliculogenesis |
| title_short | Overview of Gene Expression Dynamics during Human Oogenesis/Folliculogenesis |
| title_sort | overview of gene expression dynamics during human oogenesis folliculogenesis |
| topic | folliculogenesis maturation oocyte RNA-seq transcriptome |
| url | https://www.mdpi.com/1422-0067/25/1/33 |
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