A Novel Analysis of the Peptide Terminome Characterizes Dynamics of Proteolytic Regulation in Vertebrate Skeletal Muscle Under Severe Stress
In healthy cells, proteolysis is orderly executed to maintain basal homeostasis and normal physiology. Dyscontrol in proteolysis under severe stress condition induces cell death, but the dynamics of proteolytic regulation towards the critical phase remain unclear. Teleosts have been suggested an alt...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-02-01
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| Series: | Proteomes |
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| Online Access: | https://www.mdpi.com/2227-7382/7/1/6 |
| _version_ | 1811306537228959744 |
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| author | Yuri Kominami Tatsuya Hayashi Tetsuji Tokihiro Hideki Ushio |
| author_facet | Yuri Kominami Tatsuya Hayashi Tetsuji Tokihiro Hideki Ushio |
| author_sort | Yuri Kominami |
| collection | DOAJ |
| description | In healthy cells, proteolysis is orderly executed to maintain basal homeostasis and normal physiology. Dyscontrol in proteolysis under severe stress condition induces cell death, but the dynamics of proteolytic regulation towards the critical phase remain unclear. Teleosts have been suggested an alternative model for the study of proteolysis under severe stress. In this study, horse mackerel (<i>Trachurus japonicus</i>) was used and exacerbated under severe stress conditions due to air exposure. Although the complete genome for <i>T. japonicus</i> is not available, a transcriptomic analysis was performed to construct a reference protein database, and the expression of 72 proteases were confirmed. Quantitative peptidomic analysis revealed that proteins related to glycolysis and muscle contraction systems were highly cleaved into peptides immediately under the severe stress. Novel analysis of the peptide terminome using a multiple linear regression model demonstrated profiles of proteolysis under severe stress. The results indicated a phase transition towards dyscontrol in proteolysis in <i>T. japonicus</i> skeletal muscle during air exposure. Our novel approach will aid in investigating the dynamics of proteolytic regulation in skeletal muscle of non-model vertebrates. |
| first_indexed | 2024-04-13T08:47:14Z |
| format | Article |
| id | doaj.art-fad0c6c3358f44898edf6e2b4586ea90 |
| institution | Directory Open Access Journal |
| issn | 2227-7382 |
| language | English |
| last_indexed | 2024-04-13T08:47:14Z |
| publishDate | 2019-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Proteomes |
| spelling | doaj.art-fad0c6c3358f44898edf6e2b4586ea902022-12-22T02:53:39ZengMDPI AGProteomes2227-73822019-02-0171610.3390/proteomes7010006proteomes7010006A Novel Analysis of the Peptide Terminome Characterizes Dynamics of Proteolytic Regulation in Vertebrate Skeletal Muscle Under Severe StressYuri Kominami0Tatsuya Hayashi1Tetsuji Tokihiro2Hideki Ushio3Department of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, JapanDepartment of Mathematical Sciences, Graduate School of Mathematical Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8914, JapanDepartment of Mathematical Sciences, Graduate School of Mathematical Sciences, The University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8914, JapanDepartment of Aquatic Bioscience, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, JapanIn healthy cells, proteolysis is orderly executed to maintain basal homeostasis and normal physiology. Dyscontrol in proteolysis under severe stress condition induces cell death, but the dynamics of proteolytic regulation towards the critical phase remain unclear. Teleosts have been suggested an alternative model for the study of proteolysis under severe stress. In this study, horse mackerel (<i>Trachurus japonicus</i>) was used and exacerbated under severe stress conditions due to air exposure. Although the complete genome for <i>T. japonicus</i> is not available, a transcriptomic analysis was performed to construct a reference protein database, and the expression of 72 proteases were confirmed. Quantitative peptidomic analysis revealed that proteins related to glycolysis and muscle contraction systems were highly cleaved into peptides immediately under the severe stress. Novel analysis of the peptide terminome using a multiple linear regression model demonstrated profiles of proteolysis under severe stress. The results indicated a phase transition towards dyscontrol in proteolysis in <i>T. japonicus</i> skeletal muscle during air exposure. Our novel approach will aid in investigating the dynamics of proteolytic regulation in skeletal muscle of non-model vertebrates.https://www.mdpi.com/2227-7382/7/1/6peptide terminomeproteolysispeptidomic analysismultiple linear regression modelnon-model vertebrates |
| spellingShingle | Yuri Kominami Tatsuya Hayashi Tetsuji Tokihiro Hideki Ushio A Novel Analysis of the Peptide Terminome Characterizes Dynamics of Proteolytic Regulation in Vertebrate Skeletal Muscle Under Severe Stress Proteomes peptide terminome proteolysis peptidomic analysis multiple linear regression model non-model vertebrates |
| title | A Novel Analysis of the Peptide Terminome Characterizes Dynamics of Proteolytic Regulation in Vertebrate Skeletal Muscle Under Severe Stress |
| title_full | A Novel Analysis of the Peptide Terminome Characterizes Dynamics of Proteolytic Regulation in Vertebrate Skeletal Muscle Under Severe Stress |
| title_fullStr | A Novel Analysis of the Peptide Terminome Characterizes Dynamics of Proteolytic Regulation in Vertebrate Skeletal Muscle Under Severe Stress |
| title_full_unstemmed | A Novel Analysis of the Peptide Terminome Characterizes Dynamics of Proteolytic Regulation in Vertebrate Skeletal Muscle Under Severe Stress |
| title_short | A Novel Analysis of the Peptide Terminome Characterizes Dynamics of Proteolytic Regulation in Vertebrate Skeletal Muscle Under Severe Stress |
| title_sort | novel analysis of the peptide terminome characterizes dynamics of proteolytic regulation in vertebrate skeletal muscle under severe stress |
| topic | peptide terminome proteolysis peptidomic analysis multiple linear regression model non-model vertebrates |
| url | https://www.mdpi.com/2227-7382/7/1/6 |
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