Clinical significance and in vitro biological function of GALNS protein in hepatocellular carcinoma
Objective To investigate the clinical significance and in vitro biological function of N-acetylgalactosamine-6-sulfate sulfatase (GALNS) in hepatocellular carcinoma (HCC). Methods A retrospective cohort study was conducted on 72 HCC patients in our hospital from January 2008 to December 2014. Their...
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| Format: | Article |
| Language: | zho |
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Editorial Office of Journal of Army Medical University
2022-12-01
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| Series: | 陆军军医大学学报 |
| Subjects: | |
| Online Access: | http://aammt.tmmu.edu.cn/Upload/rhtml/202209304.htm |
| _version_ | 1797974805083848704 |
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| author | LIU Zeyu LUO Yuandeng LI Xun |
| author_facet | LIU Zeyu LUO Yuandeng LI Xun |
| author_sort | LIU Zeyu |
| collection | DOAJ |
| description | Objective To investigate the clinical significance and in vitro biological function of N-acetylgalactosamine-6-sulfate sulfatase (GALNS) in hepatocellular carcinoma (HCC). Methods A retrospective cohort study was conducted on 72 HCC patients in our hospital from January 2008 to December 2014. Their HCC tissues and para-cancerous tissues were collected and detected for GALNS expression with immunohistochemical staining. The clinical significance of GALNS in HCC was analyzed based on the results to immunohistochemical staining and clinicopathological data. The biological function of GALNS in HCC cell lines Huh7 and LM3 was detected by cell migration and proliferation assay after GALNS knockdown. Results ① In the 72 HCC tissues, there were 32 samples with high and 40 with low expression of GALNS. Its high level was associated with tumor length, TNM stage and intrahepatic metastasis (Chi-square=20.649, 14.849, 5.667, P < 0.05); ②The 1-, 3- and 5-year survival rates of the 72 patients after surgical treatment were 87.50%, 42.50% and 40.00%, respectively for those with low GALNS level, and 37.50%, 12.50% and 3.13%, for those high expression level. The survival rate was significantly better in the low expression patients than those of high level (Chi-square=9.268, P < 0.05); ③GALNS expression (HR=2.502, 95%CI=1.369- 4.572, P=0.003), distant metastasis (HR=1.908, 95%CI=1.018-3.573, P=0.044), vascular invasion (HR=2.361, 95%CI=1.267-4.398, P=0.044) P=0.007) and pathological stage (HR=2.223, 95%CI=1.185-4.171, P=0.013) were independent risk factors for HCC. ④Down-regulation of GALNS significantly inhibited the proliferation and metastasis of HCC cells (P < 0.05). Conclusion GALNS is highly expressed in liver cancer tissues and an independent risk factor for the prognosis of HCC patients. Downregulation of GALNS can inhibit the proliferation and migration of liver cancer cells.
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| first_indexed | 2024-04-11T04:24:28Z |
| format | Article |
| id | doaj.art-fad38f680285436e8d398d4b3bee0061 |
| institution | Directory Open Access Journal |
| issn | 2097-0927 |
| language | zho |
| last_indexed | 2024-04-11T04:24:28Z |
| publishDate | 2022-12-01 |
| publisher | Editorial Office of Journal of Army Medical University |
| record_format | Article |
| series | 陆军军医大学学报 |
| spelling | doaj.art-fad38f680285436e8d398d4b3bee00612022-12-30T03:03:02ZzhoEditorial Office of Journal of Army Medical University陆军军医大学学报2097-09272022-12-0144242514252210.16016/j.2097-0927.202209304Clinical significance and in vitro biological function of GALNS protein in hepatocellular carcinomaLIU Zeyu0LUO Yuandeng1LI Xun2Institute of Hepatobiliary Surgery, First Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing, 400038, ChinaInstitute of Hepatobiliary Surgery, First Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing, 400038, ChinaInstitute of Hepatobiliary Surgery, First Affiliated Hospital, Army Medical University (Third Military Medical University), Chongqing, 400038, ChinaObjective To investigate the clinical significance and in vitro biological function of N-acetylgalactosamine-6-sulfate sulfatase (GALNS) in hepatocellular carcinoma (HCC). Methods A retrospective cohort study was conducted on 72 HCC patients in our hospital from January 2008 to December 2014. Their HCC tissues and para-cancerous tissues were collected and detected for GALNS expression with immunohistochemical staining. The clinical significance of GALNS in HCC was analyzed based on the results to immunohistochemical staining and clinicopathological data. The biological function of GALNS in HCC cell lines Huh7 and LM3 was detected by cell migration and proliferation assay after GALNS knockdown. Results ① In the 72 HCC tissues, there were 32 samples with high and 40 with low expression of GALNS. Its high level was associated with tumor length, TNM stage and intrahepatic metastasis (Chi-square=20.649, 14.849, 5.667, P < 0.05); ②The 1-, 3- and 5-year survival rates of the 72 patients after surgical treatment were 87.50%, 42.50% and 40.00%, respectively for those with low GALNS level, and 37.50%, 12.50% and 3.13%, for those high expression level. The survival rate was significantly better in the low expression patients than those of high level (Chi-square=9.268, P < 0.05); ③GALNS expression (HR=2.502, 95%CI=1.369- 4.572, P=0.003), distant metastasis (HR=1.908, 95%CI=1.018-3.573, P=0.044), vascular invasion (HR=2.361, 95%CI=1.267-4.398, P=0.044) P=0.007) and pathological stage (HR=2.223, 95%CI=1.185-4.171, P=0.013) were independent risk factors for HCC. ④Down-regulation of GALNS significantly inhibited the proliferation and metastasis of HCC cells (P < 0.05). Conclusion GALNS is highly expressed in liver cancer tissues and an independent risk factor for the prognosis of HCC patients. Downregulation of GALNS can inhibit the proliferation and migration of liver cancer cells. http://aammt.tmmu.edu.cn/Upload/rhtml/202209304.htmn-acetylgalactosamine-6-sulfatasehepatocellular carcinomabiological functionprognostic markers |
| spellingShingle | LIU Zeyu LUO Yuandeng LI Xun Clinical significance and in vitro biological function of GALNS protein in hepatocellular carcinoma 陆军军医大学学报 n-acetylgalactosamine-6-sulfatase hepatocellular carcinoma biological function prognostic markers |
| title | Clinical significance and in vitro biological function of GALNS protein in hepatocellular carcinoma |
| title_full | Clinical significance and in vitro biological function of GALNS protein in hepatocellular carcinoma |
| title_fullStr | Clinical significance and in vitro biological function of GALNS protein in hepatocellular carcinoma |
| title_full_unstemmed | Clinical significance and in vitro biological function of GALNS protein in hepatocellular carcinoma |
| title_short | Clinical significance and in vitro biological function of GALNS protein in hepatocellular carcinoma |
| title_sort | clinical significance and in vitro biological function of galns protein in hepatocellular carcinoma |
| topic | n-acetylgalactosamine-6-sulfatase hepatocellular carcinoma biological function prognostic markers |
| url | http://aammt.tmmu.edu.cn/Upload/rhtml/202209304.htm |
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