Identification of Early Salinity Stress-Responsive Proteins in <i>Dunaliella salina</i> by isobaric tags for relative and absolute quantitation (iTRAQ)-Based Quantitative Proteomic Analysis
Salt stress is one of the most serious abiotic factors that inhibit plant growth. <i>Dunaliella salina</i> has been recognized as a model organism for stress response research due to its high capacity to tolerate extreme salt stress. A proteomic approach based on isobaric tags for relati...
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| Format: | Article |
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MDPI AG
2019-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/20/3/599 |
| _version_ | 1811330809111511040 |
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| author | Yuan Wang Yuting Cong Yonghua Wang Zihu Guo Jinrong Yue Zhenyu Xing Xiangnan Gao Xiaojie Chai |
| author_facet | Yuan Wang Yuting Cong Yonghua Wang Zihu Guo Jinrong Yue Zhenyu Xing Xiangnan Gao Xiaojie Chai |
| author_sort | Yuan Wang |
| collection | DOAJ |
| description | Salt stress is one of the most serious abiotic factors that inhibit plant growth. <i>Dunaliella salina</i> has been recognized as a model organism for stress response research due to its high capacity to tolerate extreme salt stress. A proteomic approach based on isobaric tags for relative and absolute quantitation (iTRAQ) was used to analyze the proteome of <i>D. salina</i> during early response to salt stress and identify the differentially abundant proteins (DAPs). A total of 141 DAPs were identified in salt-treated samples, including 75 upregulated and 66 downregulated DAPs after 3 and 24 h of salt stress. DAPs were annotated and classified into gene ontology functional groups. The Kyoto Encyclopedia of Genes and Genomes pathway analysis linked DAPs to tricarboxylic acid cycle, photosynthesis and oxidative phosphorylation. Using search tool for the retrieval of interacting genes (STRING) software, regulatory protein⁻protein interaction (PPI) networks of the DAPs containing 33 and 52 nodes were built at each time point, which showed that photosynthesis and ATP synthesis were crucial for the modulation of early salinity-responsive pathways. The corresponding transcript levels of five DAPs were quantified by quantitative real-time polymerase chain reaction (qRT-PCR). These results presented an overview of the systematic molecular response to salt stress. This study revealed a complex regulatory mechanism of early salt tolerance in <i>D. salina</i> and potentially contributes to developing strategies to improve stress resilience. |
| first_indexed | 2024-04-13T16:09:27Z |
| format | Article |
| id | doaj.art-fad4b1baa90e407d9c4db72e98e3c446 |
| institution | Directory Open Access Journal |
| issn | 1422-0067 |
| language | English |
| last_indexed | 2024-04-13T16:09:27Z |
| publishDate | 2019-01-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-fad4b1baa90e407d9c4db72e98e3c4462022-12-22T02:40:18ZengMDPI AGInternational Journal of Molecular Sciences1422-00672019-01-0120359910.3390/ijms20030599ijms20030599Identification of Early Salinity Stress-Responsive Proteins in <i>Dunaliella salina</i> by isobaric tags for relative and absolute quantitation (iTRAQ)-Based Quantitative Proteomic AnalysisYuan Wang0Yuting Cong1Yonghua Wang2Zihu Guo3Jinrong Yue4Zhenyu Xing5Xiangnan Gao6Xiaojie Chai7Key Laboratory of Hydrobiology in Liaoning Province’s Universities, Dalian Ocean University, Dalian 116021, ChinaCollege of fisheries and life science, Dalian Ocean University, Dalian 116021, ChinaBioinformatics Center, College of Life Sciences, Northwest A&F University, Yangling 712100, ChinaCollege of Life Sciences, Northwest University, Xi’an, Shaanxi 710069, ChinaCollege of fisheries and life science, Dalian Ocean University, Dalian 116021, ChinaCollege of fisheries and life science, Dalian Ocean University, Dalian 116021, ChinaCollege of fisheries and life science, Dalian Ocean University, Dalian 116021, ChinaCollege of fisheries and life science, Dalian Ocean University, Dalian 116021, ChinaSalt stress is one of the most serious abiotic factors that inhibit plant growth. <i>Dunaliella salina</i> has been recognized as a model organism for stress response research due to its high capacity to tolerate extreme salt stress. A proteomic approach based on isobaric tags for relative and absolute quantitation (iTRAQ) was used to analyze the proteome of <i>D. salina</i> during early response to salt stress and identify the differentially abundant proteins (DAPs). A total of 141 DAPs were identified in salt-treated samples, including 75 upregulated and 66 downregulated DAPs after 3 and 24 h of salt stress. DAPs were annotated and classified into gene ontology functional groups. The Kyoto Encyclopedia of Genes and Genomes pathway analysis linked DAPs to tricarboxylic acid cycle, photosynthesis and oxidative phosphorylation. Using search tool for the retrieval of interacting genes (STRING) software, regulatory protein⁻protein interaction (PPI) networks of the DAPs containing 33 and 52 nodes were built at each time point, which showed that photosynthesis and ATP synthesis were crucial for the modulation of early salinity-responsive pathways. The corresponding transcript levels of five DAPs were quantified by quantitative real-time polymerase chain reaction (qRT-PCR). These results presented an overview of the systematic molecular response to salt stress. This study revealed a complex regulatory mechanism of early salt tolerance in <i>D. salina</i> and potentially contributes to developing strategies to improve stress resilience.https://www.mdpi.com/1422-0067/20/3/599Salinity stress<i>Dunaliella salina</i>isobaric tags for relative and absolute quantitationdifferentially abundant proteinsproteomics |
| spellingShingle | Yuan Wang Yuting Cong Yonghua Wang Zihu Guo Jinrong Yue Zhenyu Xing Xiangnan Gao Xiaojie Chai Identification of Early Salinity Stress-Responsive Proteins in <i>Dunaliella salina</i> by isobaric tags for relative and absolute quantitation (iTRAQ)-Based Quantitative Proteomic Analysis International Journal of Molecular Sciences Salinity stress <i>Dunaliella salina</i> isobaric tags for relative and absolute quantitation differentially abundant proteins proteomics |
| title | Identification of Early Salinity Stress-Responsive Proteins in <i>Dunaliella salina</i> by isobaric tags for relative and absolute quantitation (iTRAQ)-Based Quantitative Proteomic Analysis |
| title_full | Identification of Early Salinity Stress-Responsive Proteins in <i>Dunaliella salina</i> by isobaric tags for relative and absolute quantitation (iTRAQ)-Based Quantitative Proteomic Analysis |
| title_fullStr | Identification of Early Salinity Stress-Responsive Proteins in <i>Dunaliella salina</i> by isobaric tags for relative and absolute quantitation (iTRAQ)-Based Quantitative Proteomic Analysis |
| title_full_unstemmed | Identification of Early Salinity Stress-Responsive Proteins in <i>Dunaliella salina</i> by isobaric tags for relative and absolute quantitation (iTRAQ)-Based Quantitative Proteomic Analysis |
| title_short | Identification of Early Salinity Stress-Responsive Proteins in <i>Dunaliella salina</i> by isobaric tags for relative and absolute quantitation (iTRAQ)-Based Quantitative Proteomic Analysis |
| title_sort | identification of early salinity stress responsive proteins in i dunaliella salina i by isobaric tags for relative and absolute quantitation itraq based quantitative proteomic analysis |
| topic | Salinity stress <i>Dunaliella salina</i> isobaric tags for relative and absolute quantitation differentially abundant proteins proteomics |
| url | https://www.mdpi.com/1422-0067/20/3/599 |
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