Systematic Evaluation of Antigenic Stimulation in Chronic Lymphocytic Leukemia: Humoral Immunity as Biomarkers for Disease Evolution
Chronic lymphocytic leukemia (CLL) is the most common leukemia in the Western world. Studies of CLL antibody reactivity have shown differential targets to autoantigens and antimicrobial molecular motifs that support the current hypothesis of CLL pathogenesis. Methods: In this study, we conducted a q...
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-01-01
|
| Series: | Cancers |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2072-6694/15/3/891 |
| _version_ | 1797625000950235136 |
|---|---|
| author | Alicia Landeira-Viñuela Miguel Alcoceba-Sanchez Almudena Navarro-Bailón Carlota Arias-Hidalgo Pablo Juanes-Velasco José Manuel Sánchez-Santos Quentin Lecrevisse Carlos Eduardo Pedreira Marina L. García-Vaquero Ángela-Patricia Hernández Enrique Montalvillo Rafael Góngora Javier De las Rivas Marcos González-Díaz Alberto Orfao Manuel Fuentes |
| author_facet | Alicia Landeira-Viñuela Miguel Alcoceba-Sanchez Almudena Navarro-Bailón Carlota Arias-Hidalgo Pablo Juanes-Velasco José Manuel Sánchez-Santos Quentin Lecrevisse Carlos Eduardo Pedreira Marina L. García-Vaquero Ángela-Patricia Hernández Enrique Montalvillo Rafael Góngora Javier De las Rivas Marcos González-Díaz Alberto Orfao Manuel Fuentes |
| author_sort | Alicia Landeira-Viñuela |
| collection | DOAJ |
| description | Chronic lymphocytic leukemia (CLL) is the most common leukemia in the Western world. Studies of CLL antibody reactivity have shown differential targets to autoantigens and antimicrobial molecular motifs that support the current hypothesis of CLL pathogenesis. Methods: In this study, we conducted a quantitative serum analysis of 7 immunoglobulins in CLL and monoclonal B-cell lymphocytosis (MBL) patients (bead-suspension protein arrays) and a serological profile (IgG and IgM) study of autoantibodies and antimicrobial antigens (protein microarrays). Results: Significant differences in the IgA levels were observed according to disease progression and evolution as well as significant alterations in IgG1 according to IGHV mutational status. More representative IgG autoantibodies in the cohort were against nonmutagenic proteins and IgM autoantibodies were against vesicle proteins. Antimicrobial IgG and IgM were detected against microbes associated with respiratory tract infections. Conclusions: Quantitative differences in immunoglobulin serum levels could be potential biomarkers for disease progression. In the top 5 tumoral antigens, we detected autoantibodies (IgM and IgG) against proteins related to cell homeostasis and metabolism in the studied cohort. The top 5 microbial antigens were associated with respiratory and gastrointestinal infections; moreover, the subsets with better prognostics were characterized by a reactivation of Cytomegalovirus. The viral humoral response could be a potential prognosis biomarker for disease progression. |
| first_indexed | 2024-03-11T09:50:39Z |
| format | Article |
| id | doaj.art-fad604c9757e46738dbe811d746d8c1e |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-11T09:50:39Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-fad604c9757e46738dbe811d746d8c1e2023-11-16T16:18:48ZengMDPI AGCancers2072-66942023-01-0115389110.3390/cancers15030891Systematic Evaluation of Antigenic Stimulation in Chronic Lymphocytic Leukemia: Humoral Immunity as Biomarkers for Disease EvolutionAlicia Landeira-Viñuela0Miguel Alcoceba-Sanchez1Almudena Navarro-Bailón2Carlota Arias-Hidalgo3Pablo Juanes-Velasco4José Manuel Sánchez-Santos5Quentin Lecrevisse6Carlos Eduardo Pedreira7Marina L. García-Vaquero8Ángela-Patricia Hernández9Enrique Montalvillo10Rafael Góngora11Javier De las Rivas12Marcos González-Díaz13Alberto Orfao14Manuel Fuentes15Department of Medicine and General Service of Cytometry, CIBERONC-CB16/12/00400, Cancer Research Centre-IBMCC, CSIC-USAL, IBSAL, Campus Miguel de Unamuno s/n, University of Salamanca-CSIC, 37008 Salamanca, SpainDepartment of Hematology, Center Research-Centre IBMCC (CSIC-USAL, IBSAL), University Hospital of Salamanca, CIBERONC-CB16/12/00233, 37007 Salamanca, SpainDepartment of Hematology, Center Research-Centre IBMCC (CSIC-USAL, IBSAL), University Hospital of Salamanca, CIBERONC-CB16/12/00233, 37007 Salamanca, SpainDepartment of Medicine and General Service of Cytometry, CIBERONC-CB16/12/00400, Cancer Research Centre-IBMCC, CSIC-USAL, IBSAL, Campus Miguel de Unamuno s/n, University of Salamanca-CSIC, 37008 Salamanca, SpainDepartment of Medicine and General Service of Cytometry, CIBERONC-CB16/12/00400, Cancer Research Centre-IBMCC, CSIC-USAL, IBSAL, Campus Miguel de Unamuno s/n, University of Salamanca-CSIC, 37008 Salamanca, SpainStatistics Department, University of Salamanca, 37008 Salamanca, SpainDepartment of Medicine and General Service of Cytometry, CIBERONC-CB16/12/00400, Cancer Research Centre-IBMCC, CSIC-USAL, IBSAL, Campus Miguel de Unamuno s/n, University of Salamanca-CSIC, 37008 Salamanca, SpainSystems and Computing Department (COPPE-PESC), Universidade Federal do Rio de Janeiro (UFRJ), Rio de Janeiro 21941-914, BrazilDepartment of Medicine and General Service of Cytometry, CIBERONC-CB16/12/00400, Cancer Research Centre-IBMCC, CSIC-USAL, IBSAL, Campus Miguel de Unamuno s/n, University of Salamanca-CSIC, 37008 Salamanca, SpainDepartment of Medicine and General Service of Cytometry, CIBERONC-CB16/12/00400, Cancer Research Centre-IBMCC, CSIC-USAL, IBSAL, Campus Miguel de Unamuno s/n, University of Salamanca-CSIC, 37008 Salamanca, SpainDepartment of Medicine and General Service of Cytometry, CIBERONC-CB16/12/00400, Cancer Research Centre-IBMCC, CSIC-USAL, IBSAL, Campus Miguel de Unamuno s/n, University of Salamanca-CSIC, 37008 Salamanca, SpainDepartment of Medicine and General Service of Cytometry, CIBERONC-CB16/12/00400, Cancer Research Centre-IBMCC, CSIC-USAL, IBSAL, Campus Miguel de Unamuno s/n, University of Salamanca-CSIC, 37008 Salamanca, SpainBioinformatics and Functional Genomics Group, Cancer Research Center (CiC-IBMCC, CSIC/USAL), Consejo Superior de Investigaciones Científicas (CSIC) and University of Salamanca (USAL), 37008 Salamanca, SpainDepartment of Hematology, Center Research-Centre IBMCC (CSIC-USAL, IBSAL), University Hospital of Salamanca, CIBERONC-CB16/12/00233, 37007 Salamanca, SpainDepartment of Medicine and General Service of Cytometry, CIBERONC-CB16/12/00400, Cancer Research Centre-IBMCC, CSIC-USAL, IBSAL, Campus Miguel de Unamuno s/n, University of Salamanca-CSIC, 37008 Salamanca, SpainDepartment of Medicine and General Service of Cytometry, CIBERONC-CB16/12/00400, Cancer Research Centre-IBMCC, CSIC-USAL, IBSAL, Campus Miguel de Unamuno s/n, University of Salamanca-CSIC, 37008 Salamanca, SpainChronic lymphocytic leukemia (CLL) is the most common leukemia in the Western world. Studies of CLL antibody reactivity have shown differential targets to autoantigens and antimicrobial molecular motifs that support the current hypothesis of CLL pathogenesis. Methods: In this study, we conducted a quantitative serum analysis of 7 immunoglobulins in CLL and monoclonal B-cell lymphocytosis (MBL) patients (bead-suspension protein arrays) and a serological profile (IgG and IgM) study of autoantibodies and antimicrobial antigens (protein microarrays). Results: Significant differences in the IgA levels were observed according to disease progression and evolution as well as significant alterations in IgG1 according to IGHV mutational status. More representative IgG autoantibodies in the cohort were against nonmutagenic proteins and IgM autoantibodies were against vesicle proteins. Antimicrobial IgG and IgM were detected against microbes associated with respiratory tract infections. Conclusions: Quantitative differences in immunoglobulin serum levels could be potential biomarkers for disease progression. In the top 5 tumoral antigens, we detected autoantibodies (IgM and IgG) against proteins related to cell homeostasis and metabolism in the studied cohort. The top 5 microbial antigens were associated with respiratory and gastrointestinal infections; moreover, the subsets with better prognostics were characterized by a reactivation of Cytomegalovirus. The viral humoral response could be a potential prognosis biomarker for disease progression.https://www.mdpi.com/2072-6694/15/3/891chronic lymphocytic leukemiaantimicrobial antibodiesautoantibodiesprotein microarrays |
| spellingShingle | Alicia Landeira-Viñuela Miguel Alcoceba-Sanchez Almudena Navarro-Bailón Carlota Arias-Hidalgo Pablo Juanes-Velasco José Manuel Sánchez-Santos Quentin Lecrevisse Carlos Eduardo Pedreira Marina L. García-Vaquero Ángela-Patricia Hernández Enrique Montalvillo Rafael Góngora Javier De las Rivas Marcos González-Díaz Alberto Orfao Manuel Fuentes Systematic Evaluation of Antigenic Stimulation in Chronic Lymphocytic Leukemia: Humoral Immunity as Biomarkers for Disease Evolution Cancers chronic lymphocytic leukemia antimicrobial antibodies autoantibodies protein microarrays |
| title | Systematic Evaluation of Antigenic Stimulation in Chronic Lymphocytic Leukemia: Humoral Immunity as Biomarkers for Disease Evolution |
| title_full | Systematic Evaluation of Antigenic Stimulation in Chronic Lymphocytic Leukemia: Humoral Immunity as Biomarkers for Disease Evolution |
| title_fullStr | Systematic Evaluation of Antigenic Stimulation in Chronic Lymphocytic Leukemia: Humoral Immunity as Biomarkers for Disease Evolution |
| title_full_unstemmed | Systematic Evaluation of Antigenic Stimulation in Chronic Lymphocytic Leukemia: Humoral Immunity as Biomarkers for Disease Evolution |
| title_short | Systematic Evaluation of Antigenic Stimulation in Chronic Lymphocytic Leukemia: Humoral Immunity as Biomarkers for Disease Evolution |
| title_sort | systematic evaluation of antigenic stimulation in chronic lymphocytic leukemia humoral immunity as biomarkers for disease evolution |
| topic | chronic lymphocytic leukemia antimicrobial antibodies autoantibodies protein microarrays |
| url | https://www.mdpi.com/2072-6694/15/3/891 |
| work_keys_str_mv | AT alicialandeiravinuela systematicevaluationofantigenicstimulationinchroniclymphocyticleukemiahumoralimmunityasbiomarkersfordiseaseevolution AT miguelalcocebasanchez systematicevaluationofantigenicstimulationinchroniclymphocyticleukemiahumoralimmunityasbiomarkersfordiseaseevolution AT almudenanavarrobailon systematicevaluationofantigenicstimulationinchroniclymphocyticleukemiahumoralimmunityasbiomarkersfordiseaseevolution AT carlotaariashidalgo systematicevaluationofantigenicstimulationinchroniclymphocyticleukemiahumoralimmunityasbiomarkersfordiseaseevolution AT pablojuanesvelasco systematicevaluationofantigenicstimulationinchroniclymphocyticleukemiahumoralimmunityasbiomarkersfordiseaseevolution AT josemanuelsanchezsantos systematicevaluationofantigenicstimulationinchroniclymphocyticleukemiahumoralimmunityasbiomarkersfordiseaseevolution AT quentinlecrevisse systematicevaluationofantigenicstimulationinchroniclymphocyticleukemiahumoralimmunityasbiomarkersfordiseaseevolution AT carloseduardopedreira systematicevaluationofantigenicstimulationinchroniclymphocyticleukemiahumoralimmunityasbiomarkersfordiseaseevolution AT marinalgarciavaquero systematicevaluationofantigenicstimulationinchroniclymphocyticleukemiahumoralimmunityasbiomarkersfordiseaseevolution AT angelapatriciahernandez systematicevaluationofantigenicstimulationinchroniclymphocyticleukemiahumoralimmunityasbiomarkersfordiseaseevolution AT enriquemontalvillo systematicevaluationofantigenicstimulationinchroniclymphocyticleukemiahumoralimmunityasbiomarkersfordiseaseevolution AT rafaelgongora systematicevaluationofantigenicstimulationinchroniclymphocyticleukemiahumoralimmunityasbiomarkersfordiseaseevolution AT javierdelasrivas systematicevaluationofantigenicstimulationinchroniclymphocyticleukemiahumoralimmunityasbiomarkersfordiseaseevolution AT marcosgonzalezdiaz systematicevaluationofantigenicstimulationinchroniclymphocyticleukemiahumoralimmunityasbiomarkersfordiseaseevolution AT albertoorfao systematicevaluationofantigenicstimulationinchroniclymphocyticleukemiahumoralimmunityasbiomarkersfordiseaseevolution AT manuelfuentes systematicevaluationofantigenicstimulationinchroniclymphocyticleukemiahumoralimmunityasbiomarkersfordiseaseevolution |