Silver nanoparticles–decorated extracellular matrix graft: fabrication and tendon reconstruction performance
Abstract Background The reconstruction of tendons with large defects requires grafts with high mechanical strength and is often hindered by complications such as infection and adhesion. Hence, grafts combining the advantages of mechanical resilience and antibacterial/antiadhesion activity are highly...
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| Format: | Article |
| Language: | English |
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American Association for the Advancement of Science (AAAS)
2023-09-01
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| Series: | Biomaterials Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40824-023-00428-0 |
| _version_ | 1827331689453453312 |
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| author | Sunfang Chen Dan Cai Qi Dong Gaoxiang Ma Chennan Xu Xiaogang Bao Wei Yuan Bing Wu Bin Fang |
| author_facet | Sunfang Chen Dan Cai Qi Dong Gaoxiang Ma Chennan Xu Xiaogang Bao Wei Yuan Bing Wu Bin Fang |
| author_sort | Sunfang Chen |
| collection | DOAJ |
| description | Abstract Background The reconstruction of tendons with large defects requires grafts with high mechanical strength and is often hindered by complications such as infection and adhesion. Hence, grafts combining the advantages of mechanical resilience and antibacterial/antiadhesion activity are highly sought after. Methods The silver nanoparticles (GA-Ag NPs) synthesized from gallic acid and silver nitrate were attached to a decellularized extracellular matrix (Decellularized Tendon crosslinking GA-AgNPs, DT-Ag). We examined the histological structure, mechanical property, morphology, Zeta potential, cytotoxicity, antibacterial properties, antioxidant and anti-inflammatory properties, and ability of the DT-Ag to treat tendon defects in animals. Results Approximately 108.57 ± 0.94 μg GA-Ag NPs loaded per 50 mg DT, the cross-linked part of GA-Ag NPs was 65.47 ± 0.57%, which provided DT-Ag with long-lasting antibacterial activity. Meanwhile, GA endowed DT-Ag with good antioxidant and anti-inflammatory activities. Additionally, The DT-Ag facilitated M2 macrophage polarization, and suppressed fibrin deposition by hindering fibroblast adhesion. Mormore, the main advantages of DT-Ag, namely its long-lasting antibacterial activity (tested using Escherichia coli and Staphylococcus aureus as models) and the ability to prevent tissue adhesion were confirmed in vivo. Conclusion The fabricated multifunctional tendon graft was highly hydrophilic, biocompatible, and mechanically resilient, and concluded to be well suited for dealing with the main complications of surgical tendon reconstruction and has bright application prospects. Graphical Abstract |
| first_indexed | 2024-03-07T16:42:51Z |
| format | Article |
| id | doaj.art-fad624828f80401dbf5f4979f625292a |
| institution | Directory Open Access Journal |
| issn | 2055-7124 |
| language | English |
| last_indexed | 2024-03-07T16:42:51Z |
| publishDate | 2023-09-01 |
| publisher | American Association for the Advancement of Science (AAAS) |
| record_format | Article |
| series | Biomaterials Research |
| spelling | doaj.art-fad624828f80401dbf5f4979f625292a2024-03-03T07:52:01ZengAmerican Association for the Advancement of Science (AAAS)Biomaterials Research2055-71242023-09-0127111910.1186/s40824-023-00428-0Silver nanoparticles–decorated extracellular matrix graft: fabrication and tendon reconstruction performanceSunfang Chen0Dan Cai1Qi Dong2Gaoxiang Ma3Chennan Xu4Xiaogang Bao5Wei Yuan6Bing Wu7Bin Fang8Department of Orthopedics, the First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine)Department of Orthopedics, the First People’s Hospital of Huzhou, First Affiliated Hospital of Huzhou UniversityDepartment of Orthopedics, Honghui Hospital, Xi’an Jiao Tong UniversityDepartment of Orthopedics, the Central Hospital Affiliated to Shaoxing UniversityDepartment of Orthopedics, the Central Hospital Affiliated to Shaoxing UniversityDepartment of Orthopedics, The Spine Surgical Center, Second Affiliated Hospital of Naval Medical UniversityDepartment of Orthopedics, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese MedicineDepartment of Orthopedics, the Central Hospital Affiliated to Shaoxing UniversityDepartment of Orthopedics, the First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine)Abstract Background The reconstruction of tendons with large defects requires grafts with high mechanical strength and is often hindered by complications such as infection and adhesion. Hence, grafts combining the advantages of mechanical resilience and antibacterial/antiadhesion activity are highly sought after. Methods The silver nanoparticles (GA-Ag NPs) synthesized from gallic acid and silver nitrate were attached to a decellularized extracellular matrix (Decellularized Tendon crosslinking GA-AgNPs, DT-Ag). We examined the histological structure, mechanical property, morphology, Zeta potential, cytotoxicity, antibacterial properties, antioxidant and anti-inflammatory properties, and ability of the DT-Ag to treat tendon defects in animals. Results Approximately 108.57 ± 0.94 μg GA-Ag NPs loaded per 50 mg DT, the cross-linked part of GA-Ag NPs was 65.47 ± 0.57%, which provided DT-Ag with long-lasting antibacterial activity. Meanwhile, GA endowed DT-Ag with good antioxidant and anti-inflammatory activities. Additionally, The DT-Ag facilitated M2 macrophage polarization, and suppressed fibrin deposition by hindering fibroblast adhesion. Mormore, the main advantages of DT-Ag, namely its long-lasting antibacterial activity (tested using Escherichia coli and Staphylococcus aureus as models) and the ability to prevent tissue adhesion were confirmed in vivo. Conclusion The fabricated multifunctional tendon graft was highly hydrophilic, biocompatible, and mechanically resilient, and concluded to be well suited for dealing with the main complications of surgical tendon reconstruction and has bright application prospects. Graphical Abstracthttps://doi.org/10.1186/s40824-023-00428-0Tendon graftExtracellular matrixSilver nanoparticlesInfectionInflammation |
| spellingShingle | Sunfang Chen Dan Cai Qi Dong Gaoxiang Ma Chennan Xu Xiaogang Bao Wei Yuan Bing Wu Bin Fang Silver nanoparticles–decorated extracellular matrix graft: fabrication and tendon reconstruction performance Biomaterials Research Tendon graft Extracellular matrix Silver nanoparticles Infection Inflammation |
| title | Silver nanoparticles–decorated extracellular matrix graft: fabrication and tendon reconstruction performance |
| title_full | Silver nanoparticles–decorated extracellular matrix graft: fabrication and tendon reconstruction performance |
| title_fullStr | Silver nanoparticles–decorated extracellular matrix graft: fabrication and tendon reconstruction performance |
| title_full_unstemmed | Silver nanoparticles–decorated extracellular matrix graft: fabrication and tendon reconstruction performance |
| title_short | Silver nanoparticles–decorated extracellular matrix graft: fabrication and tendon reconstruction performance |
| title_sort | silver nanoparticles decorated extracellular matrix graft fabrication and tendon reconstruction performance |
| topic | Tendon graft Extracellular matrix Silver nanoparticles Infection Inflammation |
| url | https://doi.org/10.1186/s40824-023-00428-0 |
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