Effect of lyophilization on HRP–antibody conjugation: an enhanced antibody labeling technology

Effect of lyophilization on HRP–antibody conjugation: an enhanced antibody labeling technology

Abstract Objective Immunoassay usually deal with the antibody labeling with various reporter molecules, one such useful reporter molecule is horseradish peroxidase (HRPO). Conjugating enzyme with antibody without losing its enzymatic activity is a challenging task. Our aim is to modify existing clas...

Detaylı Bibliyografya
Asıl Yazarlar:	Saikant Regidi, Shilpa Ravindran, Ashitha L. Vijayan, Vani Maya, Lakshmi Sreedharan, Jeslin Varghese, Kartik Ramaswami, Manoj Gopi
Materyal Türü:	Makale
Dil:	English
Baskı/Yayın Bilgisi:	BMC 2018-08-01
Seri Bilgileri:	BMC Research Notes
Konular:	Conjugation Horseradish peroxidase ELISA Immunoassay
Online Erişim:	http://link.springer.com/article/10.1186/s13104-018-3688-8

Benzer Materyaller

Discovering a Dihydrofluorescein Analogue as a Promising Fluorescence Substrate to HRP
Yazar:: Jiayan Zhu, ve diğerleri
Baskı/Yayın Bilgisi: (2023-02-01)

Comparison of two peroxidases with high potential for biotechnology applications – HRP vs. APEX2
Yazar:: Sanja Škulj, ve diğerleri
Baskı/Yayın Bilgisi: (2024-12-01)

Biocatalytic properties of horseradish root extract peroxidase (HRP)
Yazar:: D Purev, ve diğerleri
Baskı/Yayın Bilgisi: (2014-12-01)

Homologous ELISA for detection of prednisolone in human serum
Yazar:: Dinesh Kumar, ve diğerleri
Baskı/Yayın Bilgisi: (2018-01-01)

Study on the false positive problem caused by HRP-labeled secondary antibody in detecting heme binding protein
Yazar:: Yanan ZHANG, ve diğerleri
Baskı/Yayın Bilgisi: (2019-06-01)

Chemiluminescence based immunoassay for the detection of heroin and its metabolites
Yazar:: Smritee Singh, ve diğerleri
Baskı/Yayın Bilgisi: (2018-01-01)

A Simple Biosensor Based on Streptavidin-HRP for the Detection of Bacteria Exploiting HRPs Molecular Surface Properties
Yazar:: Filippo Savini, ve diğerleri
Baskı/Yayın Bilgisi: (2023-09-01)

Development of nanobody-horseradish peroxidase-based sandwich ELISA to detect Salmonella Enteritidis in milk and in vivo colonization in chicken
Yazar:: Kui Gu, ve diğerleri
Baskı/Yayın Bilgisi: (2022-03-01)

Immobilization of HRP in Mesoporous Silica and Its Application for the Construction of Polyaniline Modified Hydrogen Peroxide Biosensor
Yazar:: Chien-Chung Chen, ve diğerleri
Baskı/Yayın Bilgisi: (2009-06-01)

Stopped Flow of Glycerol Induces the Enhancement of Adsorption and Aggregation of HRP on Mica
Yazar:: Yuri D. Ivanov, ve diğerleri
Baskı/Yayın Bilgisi: (2023-05-01)

A Novel Highly Sensitive Chemiluminescence Enzyme Immunoassay with Signal Enhancement Using Horseradish Peroxidase-Luminol-Hydrogen Peroxide Reaction for the Quantitation of Monoclonal Antibodies Used for Cancer Immunotherapy
Yazar:: Ibrahim A. Darwish, ve diğerleri
Baskı/Yayın Bilgisi: (2023-04-01)

Competitive HRP-Linked Colorimetric Aptasensor for the Detection of Fumonisin B1 in Food based on Dual Biotin-Streptavidin Interaction
Yazar:: Zui Tao, ve diğerleri
Baskı/Yayın Bilgisi: (2020-03-01)

Design of a Boron-Doped Diamond Microcell Grafted with HRP for the Sensitive and Selective Detection of Ochratoxin A
Yazar:: Amani Chrouda, ve diğerleri
Baskı/Yayın Bilgisi: (2023-03-01)

Efficacy of Horseradish Peroxidase (HRP) Enzyme Process and H2O2 in Removal of Linear Alkyl Benzene Sulfonate (LAS) from Aqueous Solution
Yazar:: Fathollah Gholami-Borujeni, ve diğerleri
Baskı/Yayın Bilgisi: (2018-02-01)

Ficus sycomorus latex: An efficient alternative Egyptian source for horseradish peroxidase in labeling with antibodies for immunodiagnostic kits
Yazar:: Azza M. Abdel-Aty, ve diğerleri
Baskı/Yayın Bilgisi: (2018-10-01)

Characterization of some psorosis and concave gum isolates from northwestern Argentina Caracterización de aislamientos de psorosis y concave gum del noroeste argentino
Yazar:: Julia Figueroa, ve diğerleri
Baskı/Yayın Bilgisi: (2009-01-01)

Development and optimization of an in-house heterologous ELISA for detection of prednisolone drug in enzyme conjugates using spacers
Yazar:: Dinesh Kumar, ve diğerleri
Baskı/Yayın Bilgisi: (2023-08-01)

Enhanced Immobilization of Enzymes on Plasma Micro-Nanotextured Surfaces and Microfluidics: Application to HRP
Yazar:: Stefania Vorvi, ve diğerleri
Baskı/Yayın Bilgisi: (2024-10-01)

Immobilization of peroxidase from fresh horseradish extract for anthraquinone dye decolorization
Yazar:: Nataša Ž. Šekuljica, ve diğerleri
Baskı/Yayın Bilgisi: (2015-09-01)

Immobilization of horseradish peroxidase onto kaolin by glutaraldehyde method and its application in decolorization of anthraquinone dye
Yazar:: Šekuljica Nataša Ž., ve diğerleri
Baskı/Yayın Bilgisi: (2016-01-01)

IMOBILIZAÇÃO DE PEROXIDASE DE RAIZ FORTE EM BAGAÇO DE CANA-DE-AÇÚCAR
Yazar:: Monna Lisa B. Queiroz, ve diğerleri

Significance of hepatitis C virus (HCV) viremia in anti-HCV antibody (IgG) positive subjects
Yazar:: Sukrutha Gopal, ve diğerleri
Baskı/Yayın Bilgisi: (2017-01-01)

A Sandwich Immunosorbent Assay for 3-Amino-2-Oxazolidinone Following Derivatization
Yazar:: ZHANG Shiwei, WU Huiling, ZHOU Yingchun, WANG Bingzhi, YANG Xingxing, DU Yegang, TANG Lu, FENG Ronghu, GUO Jiping
Baskı/Yayın Bilgisi: (2024-03-01)

Response of letter to the editor on Procalcitonin: a promising diagnostic marker for sepsis and antibiotic therapy
Yazar:: R. Saikant, ve diğerleri
Baskı/Yayın Bilgisi: (2017-12-01)

Biochemical properties of immobilized horseradish peroxidase on ceramic and its application in removal of azo dye
Yazar:: Hala A. Salah, ve diğerleri
Baskı/Yayın Bilgisi: (2024-11-01)

Polymeric Forms of Plant Flavonoids Obtained by Enzymatic Reactions
Yazar:: Malgorzata Latos-Brozio, ve diğerleri
Baskı/Yayın Bilgisi: (2022-06-01)

A Biosensor Based on Immobilization of Horseradish Peroxidase in Chitosan Matrix Cross-linked with Glyoxal for Amperometric Determination of Hydrogen Peroxide
Yazar:: Gui-Xiang Wang, ve diğerleri
Baskı/Yayın Bilgisi: (2005-05-01)

Asymmetric Biomimetic Oxidations of Phenols: The Mechanism of the Diastereo- and Enantioselective Synthesis of Thomasidioic Acid
Yazar:: Bruno Rindone, ve diğerleri
Baskı/Yayın Bilgisi: (2008-01-01)

Multicommutated Flow Analysis System for Determination of Horseradish Peroxidase and Its Inhibitors
Yazar:: Justyna Głowacka, ve diğerleri
Baskı/Yayın Bilgisi: (2021-09-01)

Phenol Removal by Immobilized Horseradish Peroxidase
Yazar:: Iran Alemzadeh, ve diğerleri
Baskı/Yayın Bilgisi: (2010-06-01)

Investigation of the Properties of Immobilized Horseradish Peroxidase on Magnetic Particles
Yazar:: Ivanov T., ve diğerleri
Baskı/Yayın Bilgisi: (2008-12-01)

Molecular Assembly of a Durable HRP-AuNPs/PEDOT:BSA/Pt Biosensor with Detailed Characterizations
Yazar:: Fangcheng Xu, ve diğerleri
Baskı/Yayın Bilgisi: (2018-06-01)

Highly Sensitive Measurement of Horseradish Peroxidase Using Surface-Enhanced Raman Scattering of 2,3-Diaminophenazine
Yazar:: Evgeniy G. Evtushenko, ve diğerleri
Baskı/Yayın Bilgisi: (2024-02-01)

Tuning Myogenesis by Controlling Gelatin Hydrogel Properties through Hydrogen Peroxide-Mediated Cross-Linking and Degradation
Yazar:: Wildan Mubarok, ve diğerleri
Baskı/Yayın Bilgisi: (2022-06-01)

Horseradish Peroxidase (HRP) Immobilized Poly(aniline-co-m-aminophenol) Film ElectrodesÃ¢Â€Â“fabrication and Evaluation as Hydrogen Peroxide Sensor
Yazar:: Seong-Ho Choi, ve diğerleri
Baskı/Yayın Bilgisi: (2007-05-01)

Hierarchical HRP-Crosslinked Silk Fibroin/ZnSr-TCP Scaffolds for Osteochondral Tissue Regeneration: Assessment of the Mechanical and Antibacterial Properties
Yazar:: Viviana P. Ribeiro, ve diğerleri
Baskı/Yayın Bilgisi: (2020-03-01)

Modulation of Cell-Cycle Progression by Hydrogen Peroxide-Mediated Cross-Linking and Degradation of Cell-Adhesive Hydrogels
Yazar:: Wildan Mubarok, ve diğerleri
Baskı/Yayın Bilgisi: (2022-03-01)

Evaluation of activity of peroxidases contained in various plant sources
Yazar:: Solovyeva, Anna A., ve diğerleri
Baskı/Yayın Bilgisi: (2023-12-01)

Hydrogen Peroxide Sensing Based on Inner Surfaces Modification of Solid-State Nanopore
Yazar:: Libo Zhu, ve diğerleri
Baskı/Yayın Bilgisi: (2017-06-01)

Enzymatic Platforms for Sensitive Neurotransmitter Detection
Yazar:: Sylwia Baluta, ve diğerleri
Baskı/Yayın Bilgisi: (2020-01-01)