High Throughput Sequencing for Clinical Tuberculosis: An Overview
High throughput sequencing (HTS) can identify the presence of <i>Mycobacterium tuberculosis</i> DNA in a clinical sample while also providing information on drug susceptibility. Multiple studies have provided a context for exploring the clinical application of HTS for TB diagnosis. The w...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Pathogens |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-0817/11/11/1343 |
| _version_ | 1827643972891181056 |
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| author | Tara E. Ness Andrew DiNardo Maha R. Farhat |
| author_facet | Tara E. Ness Andrew DiNardo Maha R. Farhat |
| author_sort | Tara E. Ness |
| collection | DOAJ |
| description | High throughput sequencing (HTS) can identify the presence of <i>Mycobacterium tuberculosis</i> DNA in a clinical sample while also providing information on drug susceptibility. Multiple studies have provided a context for exploring the clinical application of HTS for TB diagnosis. The workflow challenges, strengths and limitations of the various sequencing platforms, and tools used for analysis are presented to provide a framework for further innovations in the field. |
| first_indexed | 2024-03-09T18:05:34Z |
| format | Article |
| id | doaj.art-fad81b8ab7c7426b8ebf88a94c59ae9b |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T18:05:34Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-fad81b8ab7c7426b8ebf88a94c59ae9b2023-11-24T09:34:11ZengMDPI AGPathogens2076-08172022-11-011111134310.3390/pathogens11111343High Throughput Sequencing for Clinical Tuberculosis: An OverviewTara E. Ness0Andrew DiNardo1Maha R. Farhat2Division of Pediatric Infectious Diseases, Baylor College of Medicine/Texas Children’s Hospital, Houston, TX 77030, USAGlobal TB Program, Baylor College of Medicine/Texas Childrens Hospital, Houston, TX 77030, USAHarvard Medical School Biomedical Informatics and Pulmonary and Critical Care Massachusetts General Hospital, Boston, MA 02115, USAHigh throughput sequencing (HTS) can identify the presence of <i>Mycobacterium tuberculosis</i> DNA in a clinical sample while also providing information on drug susceptibility. Multiple studies have provided a context for exploring the clinical application of HTS for TB diagnosis. The workflow challenges, strengths and limitations of the various sequencing platforms, and tools used for analysis are presented to provide a framework for further innovations in the field.https://www.mdpi.com/2076-0817/11/11/1343tuberculosis<i>Mycobacterium tuberculosis</i>sequencinghigh throughput sequencingnext generation sequencing |
| spellingShingle | Tara E. Ness Andrew DiNardo Maha R. Farhat High Throughput Sequencing for Clinical Tuberculosis: An Overview Pathogens tuberculosis <i>Mycobacterium tuberculosis</i> sequencing high throughput sequencing next generation sequencing |
| title | High Throughput Sequencing for Clinical Tuberculosis: An Overview |
| title_full | High Throughput Sequencing for Clinical Tuberculosis: An Overview |
| title_fullStr | High Throughput Sequencing for Clinical Tuberculosis: An Overview |
| title_full_unstemmed | High Throughput Sequencing for Clinical Tuberculosis: An Overview |
| title_short | High Throughput Sequencing for Clinical Tuberculosis: An Overview |
| title_sort | high throughput sequencing for clinical tuberculosis an overview |
| topic | tuberculosis <i>Mycobacterium tuberculosis</i> sequencing high throughput sequencing next generation sequencing |
| url | https://www.mdpi.com/2076-0817/11/11/1343 |
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