Tracking Antimicrobial Resistant <i>E. coli</i> from Pigs on Farm to Pork at Slaughter
Antimicrobial-resistant bacteria might be transferred via the foodchain. However, that risk is rarely tracked along different production steps, e.g., from pigs at farm to meat. To close that gap, we performed a prospective study in four conventional and two organic farms from the moment pigs entered...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-07-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/8/1485 |
| _version_ | 1797408820248444928 |
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| author | Rupert Bassitta Hanna Kronfeld Johann Bauer Karin Schwaiger Christina Hölzel |
| author_facet | Rupert Bassitta Hanna Kronfeld Johann Bauer Karin Schwaiger Christina Hölzel |
| author_sort | Rupert Bassitta |
| collection | DOAJ |
| description | Antimicrobial-resistant bacteria might be transferred via the foodchain. However, that risk is rarely tracked along different production steps, e.g., from pigs at farm to meat. To close that gap, we performed a prospective study in four conventional and two organic farms from the moment pigs entered the farm until meat sampling at slaughter. Antimicrobial use was recorded (0 to 11 agents). Antimicrobial susceptibility (AMS) against 26 antibiotics, including critically important substances, was tested by microdilution, and <i>tet</i>A-<i>tet</i>B-<i>sul</i>I-<i>sul</i>II-<i>str</i>A-<i>str</i>B-<i>bla</i>-CTXM-<i>qac</i>EΔ1 were included in PCR-genotyping. From 244 meat samples of 122 pigs, 54 samples (22.1%) from 45 animals were positive for <i>E. coli</i> (<i>n</i> = 198). MICs above the breakpoint/ECOFF occurred for all antibiotics except meropenem. One isolate from organic farming was markedly resistant against beta-lactams including fourth-generation cefalosporines. AMS patterns differed remarkably between isolates from one piece of meat, varying from monoresistance to 16-fold multiresistance. Amplicon-typing revealed high similarity between isolates at slaughter and on farm. Prior pig lots andeven the farmer might serve as reservoirs for <i>E. coli</i> isolated from meat at slaughter. However, AMS phenotyping and genotyping indicate that antimicrobial resistance in <i>E. coli</i> is highly dynamic, impairing reliable prediction of health risks from findings along the production chain. |
| first_indexed | 2024-03-09T04:05:07Z |
| format | Article |
| id | doaj.art-fadaee3fd678453da6b0f880bf8087c6 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T04:05:07Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-fadaee3fd678453da6b0f880bf8087c62023-12-03T14:08:45ZengMDPI AGMicroorganisms2076-26072022-07-01108148510.3390/microorganisms10081485Tracking Antimicrobial Resistant <i>E. coli</i> from Pigs on Farm to Pork at SlaughterRupert Bassitta0Hanna Kronfeld1Johann Bauer2Karin Schwaiger3Christina Hölzel4Former Department of Animal Hygiene, Technical University of Munich, Weihenstephaner Berg 3, 85354 Freising, GermanyDepartment for Animal Hygiene, Animal Health and Food Safety, Institute of Animal Breeding and Husbandry, Christian-Albrechts-University, Olshausenstr. 40, 24098 Kiel, GermanyFormer Department of Animal Hygiene, Technical University of Munich, Weihenstephaner Berg 3, 85354 Freising, GermanyUnit of Food Hygiene and Technology, Institute of Food Safety, Food Technology and Veterinary Public Health, University of Veterinary Medicine, Veterinärplatz 1, 1210 Vienna, AustriaDepartment for Animal Hygiene, Animal Health and Food Safety, Institute of Animal Breeding and Husbandry, Christian-Albrechts-University, Olshausenstr. 40, 24098 Kiel, GermanyAntimicrobial-resistant bacteria might be transferred via the foodchain. However, that risk is rarely tracked along different production steps, e.g., from pigs at farm to meat. To close that gap, we performed a prospective study in four conventional and two organic farms from the moment pigs entered the farm until meat sampling at slaughter. Antimicrobial use was recorded (0 to 11 agents). Antimicrobial susceptibility (AMS) against 26 antibiotics, including critically important substances, was tested by microdilution, and <i>tet</i>A-<i>tet</i>B-<i>sul</i>I-<i>sul</i>II-<i>str</i>A-<i>str</i>B-<i>bla</i>-CTXM-<i>qac</i>EΔ1 were included in PCR-genotyping. From 244 meat samples of 122 pigs, 54 samples (22.1%) from 45 animals were positive for <i>E. coli</i> (<i>n</i> = 198). MICs above the breakpoint/ECOFF occurred for all antibiotics except meropenem. One isolate from organic farming was markedly resistant against beta-lactams including fourth-generation cefalosporines. AMS patterns differed remarkably between isolates from one piece of meat, varying from monoresistance to 16-fold multiresistance. Amplicon-typing revealed high similarity between isolates at slaughter and on farm. Prior pig lots andeven the farmer might serve as reservoirs for <i>E. coli</i> isolated from meat at slaughter. However, AMS phenotyping and genotyping indicate that antimicrobial resistance in <i>E. coli</i> is highly dynamic, impairing reliable prediction of health risks from findings along the production chain.https://www.mdpi.com/2076-2607/10/8/1485<i>Escherichia coli</i>foodborne pathogensantimicrobial resistanceantibioticsfood chainfood safety |
| spellingShingle | Rupert Bassitta Hanna Kronfeld Johann Bauer Karin Schwaiger Christina Hölzel Tracking Antimicrobial Resistant <i>E. coli</i> from Pigs on Farm to Pork at Slaughter Microorganisms <i>Escherichia coli</i> foodborne pathogens antimicrobial resistance antibiotics food chain food safety |
| title | Tracking Antimicrobial Resistant <i>E. coli</i> from Pigs on Farm to Pork at Slaughter |
| title_full | Tracking Antimicrobial Resistant <i>E. coli</i> from Pigs on Farm to Pork at Slaughter |
| title_fullStr | Tracking Antimicrobial Resistant <i>E. coli</i> from Pigs on Farm to Pork at Slaughter |
| title_full_unstemmed | Tracking Antimicrobial Resistant <i>E. coli</i> from Pigs on Farm to Pork at Slaughter |
| title_short | Tracking Antimicrobial Resistant <i>E. coli</i> from Pigs on Farm to Pork at Slaughter |
| title_sort | tracking antimicrobial resistant i e coli i from pigs on farm to pork at slaughter |
| topic | <i>Escherichia coli</i> foodborne pathogens antimicrobial resistance antibiotics food chain food safety |
| url | https://www.mdpi.com/2076-2607/10/8/1485 |
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