Humoral immune and adjuvant responses of mucosally-administered Tinospora cordifolia immunomodulatory protein in BALB/c mice
Background: In traditional medicine, guduchi (Tinospora cordifolia) is considered as an adaptogen with immunomodulatory prowess. A 25 kDa protein from guduchi stem has been characterized as an immunomodulatory protein (ImP). Objectives: The aim of this study was to evaluate the intrinsic immunogenic...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-04-01
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| Series: | Journal of Ayurveda and Integrative Medicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0975947617301195 |
| _version_ | 1811328752999727104 |
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| author | Ivan Aranha Yeldur P. Venkatesh |
| author_facet | Ivan Aranha Yeldur P. Venkatesh |
| author_sort | Ivan Aranha |
| collection | DOAJ |
| description | Background: In traditional medicine, guduchi (Tinospora cordifolia) is considered as an adaptogen with immunomodulatory prowess. A 25 kDa protein from guduchi stem has been characterized as an immunomodulatory protein (ImP). Objectives: The aim of this study was to evaluate the intrinsic immunogenicity of guduchi ImP and adjuvant activity using ovalbumin (OVA) as antigen in BALB/c mice. Materials and Methods: Mice were given guduchi ImP (30 and 60 μg) by intranasal administration to respective groups (n = 6) on days 1, 14 and thereafter weekly till day 42. Immunogenic response was monitored by serum IgG/IgA levels (days 14, 35 and 50). The adjuvant activity was measured by serum anti-OVA IgG/IgA responses to administration of 30 μg OVA with guduchi ImP. The effect of guduchi ImP on the spleen status was examined by splenic weight (day 50). Results: Guduchi ImP administration displayed a significant increase in anti-guduchi ImP IgG (5–7 fold) and anti-guduchi ImP IgA (3–4 fold) on day 50 vs. control. Guduchi ImP showed a significant increase in anti-OVA IgG (6–7 fold) and anti-OVA IgA (4–5 fold) on day 50 vs. control. The splenic index of guduchi ImP group increased significantly in both the immune and adjuvant response groups; however, the splenic index in the adjuvant response group was markedly higher. Conclusion: The results indicate that guduchi ImP is a strong immunogen by itself and enhances the immunogenicity of mucosally-administered antigen in BALB/c mice. Based on the results of this animal study, it appears that guduchi ImP shows a potential for future studies in humans. |
| first_indexed | 2024-04-13T15:30:33Z |
| format | Article |
| id | doaj.art-fadc7c2d888849eba3724712aba0653f |
| institution | Directory Open Access Journal |
| issn | 0975-9476 |
| language | English |
| last_indexed | 2024-04-13T15:30:33Z |
| publishDate | 2020-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Ayurveda and Integrative Medicine |
| spelling | doaj.art-fadc7c2d888849eba3724712aba0653f2022-12-22T02:41:23ZengElsevierJournal of Ayurveda and Integrative Medicine0975-94762020-04-01112140146Humoral immune and adjuvant responses of mucosally-administered Tinospora cordifolia immunomodulatory protein in BALB/c miceIvan Aranha0Yeldur P. Venkatesh1Department of Biochemistry and Nutrition, CSIR–Central Food Technological Research Institute (CFTRI), Mysuru, Karnataka, IndiaCorresponding author.; Department of Biochemistry and Nutrition, CSIR–Central Food Technological Research Institute (CFTRI), Mysuru, Karnataka, IndiaBackground: In traditional medicine, guduchi (Tinospora cordifolia) is considered as an adaptogen with immunomodulatory prowess. A 25 kDa protein from guduchi stem has been characterized as an immunomodulatory protein (ImP). Objectives: The aim of this study was to evaluate the intrinsic immunogenicity of guduchi ImP and adjuvant activity using ovalbumin (OVA) as antigen in BALB/c mice. Materials and Methods: Mice were given guduchi ImP (30 and 60 μg) by intranasal administration to respective groups (n = 6) on days 1, 14 and thereafter weekly till day 42. Immunogenic response was monitored by serum IgG/IgA levels (days 14, 35 and 50). The adjuvant activity was measured by serum anti-OVA IgG/IgA responses to administration of 30 μg OVA with guduchi ImP. The effect of guduchi ImP on the spleen status was examined by splenic weight (day 50). Results: Guduchi ImP administration displayed a significant increase in anti-guduchi ImP IgG (5–7 fold) and anti-guduchi ImP IgA (3–4 fold) on day 50 vs. control. Guduchi ImP showed a significant increase in anti-OVA IgG (6–7 fold) and anti-OVA IgA (4–5 fold) on day 50 vs. control. The splenic index of guduchi ImP group increased significantly in both the immune and adjuvant response groups; however, the splenic index in the adjuvant response group was markedly higher. Conclusion: The results indicate that guduchi ImP is a strong immunogen by itself and enhances the immunogenicity of mucosally-administered antigen in BALB/c mice. Based on the results of this animal study, it appears that guduchi ImP shows a potential for future studies in humans.http://www.sciencedirect.com/science/article/pii/S0975947617301195Adjuvant activityGuduchiImmunogenic responseImmunomodulatory proteinSplenic indexTinospora cordifolia |
| spellingShingle | Ivan Aranha Yeldur P. Venkatesh Humoral immune and adjuvant responses of mucosally-administered Tinospora cordifolia immunomodulatory protein in BALB/c mice Journal of Ayurveda and Integrative Medicine Adjuvant activity Guduchi Immunogenic response Immunomodulatory protein Splenic index Tinospora cordifolia |
| title | Humoral immune and adjuvant responses of mucosally-administered Tinospora cordifolia immunomodulatory protein in BALB/c mice |
| title_full | Humoral immune and adjuvant responses of mucosally-administered Tinospora cordifolia immunomodulatory protein in BALB/c mice |
| title_fullStr | Humoral immune and adjuvant responses of mucosally-administered Tinospora cordifolia immunomodulatory protein in BALB/c mice |
| title_full_unstemmed | Humoral immune and adjuvant responses of mucosally-administered Tinospora cordifolia immunomodulatory protein in BALB/c mice |
| title_short | Humoral immune and adjuvant responses of mucosally-administered Tinospora cordifolia immunomodulatory protein in BALB/c mice |
| title_sort | humoral immune and adjuvant responses of mucosally administered tinospora cordifolia immunomodulatory protein in balb c mice |
| topic | Adjuvant activity Guduchi Immunogenic response Immunomodulatory protein Splenic index Tinospora cordifolia |
| url | http://www.sciencedirect.com/science/article/pii/S0975947617301195 |
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