Cumulus Extracellular Matrix Is an Important Part of Oocyte Microenvironment in Ovarian Follicles: Its Remodeling and Proteolytic Degradation
The extracellular matrix (ECM) is an essential structure with biological activities. It has been shown that the ECM influences gene expression via cytoskeletal components and the gene expression is dependent upon cell interactions with molecules and hormones. The development of ovarian follicles is...
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| Language: | English |
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MDPI AG
2021-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/1/54 |
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| author | Eva Nagyová Lucie Němcová Antonella Camaioni |
| author_facet | Eva Nagyová Lucie Němcová Antonella Camaioni |
| author_sort | Eva Nagyová |
| collection | DOAJ |
| description | The extracellular matrix (ECM) is an essential structure with biological activities. It has been shown that the ECM influences gene expression via cytoskeletal components and the gene expression is dependent upon cell interactions with molecules and hormones. The development of ovarian follicles is a hormone dependent process. The surge in the luteinizing hormone triggers ovulatory changes in oocyte microenvironment. In this review, we discuss how proteolytic cleavage affects formation of cumulus ECM following hormonal stimulation; in particular, how the specific proteasome inhibitor MG132 affects gonadotropin-induced cytoskeletal structure, the organization of cumulus ECM, steroidogenesis, and nuclear maturation. We found that after the inhibition of proteolytic cleavage, gonadotropin-stimulated oocyte–cumulus complexes (OCCs) were without any signs of cumulus expansion; they remained compact with preserved cytoskeletal F-actin-rich transzonal projections through the oocyte investments. Concomitantly, a significant decrease was detected in progesterone secretion and in the expression of gonadotropin-stimulated cumulus expansion–related transcripts, such as <i>HAS2</i> and <i>TNFAIP6</i>. In agreement, the covalent binding between hyaluronan and the heavy chains of serum-derived the inter-alpha-trypsin inhibitor, essential for the organization of cumulus ECM, was missing. |
| first_indexed | 2024-03-10T03:41:06Z |
| format | Article |
| id | doaj.art-fadd19c4891842a1b9cd71c22c1717a2 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T03:41:06Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-fadd19c4891842a1b9cd71c22c1717a22023-11-23T11:33:53ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-12-012315410.3390/ijms23010054Cumulus Extracellular Matrix Is an Important Part of Oocyte Microenvironment in Ovarian Follicles: Its Remodeling and Proteolytic DegradationEva Nagyová0Lucie Němcová1Antonella Camaioni2Institute of Animal Physiology and Genetics, Czech Academy of Sciences, 27721 Libechov, Czech RepublicInstitute of Animal Physiology and Genetics, Czech Academy of Sciences, 27721 Libechov, Czech RepublicDepartment of Biomedicine and Prevention, University of Rome Tor Vergata, Via Montpelier 1, 00133 Rome, ItalyThe extracellular matrix (ECM) is an essential structure with biological activities. It has been shown that the ECM influences gene expression via cytoskeletal components and the gene expression is dependent upon cell interactions with molecules and hormones. The development of ovarian follicles is a hormone dependent process. The surge in the luteinizing hormone triggers ovulatory changes in oocyte microenvironment. In this review, we discuss how proteolytic cleavage affects formation of cumulus ECM following hormonal stimulation; in particular, how the specific proteasome inhibitor MG132 affects gonadotropin-induced cytoskeletal structure, the organization of cumulus ECM, steroidogenesis, and nuclear maturation. We found that after the inhibition of proteolytic cleavage, gonadotropin-stimulated oocyte–cumulus complexes (OCCs) were without any signs of cumulus expansion; they remained compact with preserved cytoskeletal F-actin-rich transzonal projections through the oocyte investments. Concomitantly, a significant decrease was detected in progesterone secretion and in the expression of gonadotropin-stimulated cumulus expansion–related transcripts, such as <i>HAS2</i> and <i>TNFAIP6</i>. In agreement, the covalent binding between hyaluronan and the heavy chains of serum-derived the inter-alpha-trypsin inhibitor, essential for the organization of cumulus ECM, was missing.https://www.mdpi.com/1422-0067/23/1/54oocyte–cumulus complexextracellular matrixhyaluronanproteasome |
| spellingShingle | Eva Nagyová Lucie Němcová Antonella Camaioni Cumulus Extracellular Matrix Is an Important Part of Oocyte Microenvironment in Ovarian Follicles: Its Remodeling and Proteolytic Degradation International Journal of Molecular Sciences oocyte–cumulus complex extracellular matrix hyaluronan proteasome |
| title | Cumulus Extracellular Matrix Is an Important Part of Oocyte Microenvironment in Ovarian Follicles: Its Remodeling and Proteolytic Degradation |
| title_full | Cumulus Extracellular Matrix Is an Important Part of Oocyte Microenvironment in Ovarian Follicles: Its Remodeling and Proteolytic Degradation |
| title_fullStr | Cumulus Extracellular Matrix Is an Important Part of Oocyte Microenvironment in Ovarian Follicles: Its Remodeling and Proteolytic Degradation |
| title_full_unstemmed | Cumulus Extracellular Matrix Is an Important Part of Oocyte Microenvironment in Ovarian Follicles: Its Remodeling and Proteolytic Degradation |
| title_short | Cumulus Extracellular Matrix Is an Important Part of Oocyte Microenvironment in Ovarian Follicles: Its Remodeling and Proteolytic Degradation |
| title_sort | cumulus extracellular matrix is an important part of oocyte microenvironment in ovarian follicles its remodeling and proteolytic degradation |
| topic | oocyte–cumulus complex extracellular matrix hyaluronan proteasome |
| url | https://www.mdpi.com/1422-0067/23/1/54 |
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