Bile acids: analysis in biological fluids and tissues
The formation of bile acids/bile alcohols is of major importance for the maintenance of cholesterol homeostasis. Besides their functions in lipid absorption, bile acids/bile alcohols are regulatory molecules for a number of metabolic processes. Their effects are structure-dependent, and numerous met...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2010-01-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520313730 |
| _version_ | 1818658440127447040 |
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| author | William J. Griffiths Jan Sjövall |
| author_facet | William J. Griffiths Jan Sjövall |
| author_sort | William J. Griffiths |
| collection | DOAJ |
| description | The formation of bile acids/bile alcohols is of major importance for the maintenance of cholesterol homeostasis. Besides their functions in lipid absorption, bile acids/bile alcohols are regulatory molecules for a number of metabolic processes. Their effects are structure-dependent, and numerous metabolic conversions result in a complex mixture of biologically active and inactive forms. Advanced methods are required to characterize and quantify individual bile acids in these mixtures. A combination of such analyses with analyses of the proteome will be required for a better understanding of mechanisms of action and nature of endogenous ligands. Mass spectrometry is the basic detection technique for effluents from chromatographic columns. Capillary liquid chromatography-mass spectrometry with electrospray ionization provides the highest sensitivity in metabolome analysis. Classical gas chromatography-mass spectrometry is less sensitive but offers extensive structure-dependent fragmentation increasing the specificity in analyses of isobaric isomers of unconjugated bile acids. Depending on the nature of the bile acid/bile alcohol mixture and the range of concentration of individuals, different sample preparation sequences, from simple extractions to group separations and derivatizations, are applicable. We review the methods currently available for the analysis of bile acids in biological fluids and tissues, with emphasis on the combination of liquid and gas phase chromatography with mass spectrometry. |
| first_indexed | 2024-12-17T03:57:24Z |
| format | Article |
| id | doaj.art-fadd24849b864fda81f34717eb652850 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T03:57:24Z |
| publishDate | 2010-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-fadd24849b864fda81f34717eb6528502022-12-21T22:04:35ZengElsevierJournal of Lipid Research0022-22752010-01-015112341Bile acids: analysis in biological fluids and tissuesWilliam J. Griffiths0Jan Sjövall1Institute of Mass Spectrometry, School of Medicine, Grove Building Swansea University Singleton Park Swansea SA2 8PP United KingdomTo whom correspondence should be addressed; Department of Medical Biochemistry and Biophysics, Karolinska Institutet SE-17177 Stockholm SwedenThe formation of bile acids/bile alcohols is of major importance for the maintenance of cholesterol homeostasis. Besides their functions in lipid absorption, bile acids/bile alcohols are regulatory molecules for a number of metabolic processes. Their effects are structure-dependent, and numerous metabolic conversions result in a complex mixture of biologically active and inactive forms. Advanced methods are required to characterize and quantify individual bile acids in these mixtures. A combination of such analyses with analyses of the proteome will be required for a better understanding of mechanisms of action and nature of endogenous ligands. Mass spectrometry is the basic detection technique for effluents from chromatographic columns. Capillary liquid chromatography-mass spectrometry with electrospray ionization provides the highest sensitivity in metabolome analysis. Classical gas chromatography-mass spectrometry is less sensitive but offers extensive structure-dependent fragmentation increasing the specificity in analyses of isobaric isomers of unconjugated bile acids. Depending on the nature of the bile acid/bile alcohol mixture and the range of concentration of individuals, different sample preparation sequences, from simple extractions to group separations and derivatizations, are applicable. We review the methods currently available for the analysis of bile acids in biological fluids and tissues, with emphasis on the combination of liquid and gas phase chromatography with mass spectrometry.http://www.sciencedirect.com/science/article/pii/S0022227520313730liquid chromatography-mass spectrometryelectrospray ionizationgas-liquid chromatography-mass spectrometrysample preparation |
| spellingShingle | William J. Griffiths Jan Sjövall Bile acids: analysis in biological fluids and tissues Journal of Lipid Research liquid chromatography-mass spectrometry electrospray ionization gas-liquid chromatography-mass spectrometry sample preparation |
| title | Bile acids: analysis in biological fluids and tissues |
| title_full | Bile acids: analysis in biological fluids and tissues |
| title_fullStr | Bile acids: analysis in biological fluids and tissues |
| title_full_unstemmed | Bile acids: analysis in biological fluids and tissues |
| title_short | Bile acids: analysis in biological fluids and tissues |
| title_sort | bile acids analysis in biological fluids and tissues |
| topic | liquid chromatography-mass spectrometry electrospray ionization gas-liquid chromatography-mass spectrometry sample preparation |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520313730 |
| work_keys_str_mv | AT williamjgriffiths bileacidsanalysisinbiologicalfluidsandtissues AT jansjovall bileacidsanalysisinbiologicalfluidsandtissues |