Optimized workflow for human PBMC multiomic immunosurveillance studies

Optimized workflow for human PBMC multiomic immunosurveillance studies

Summary: Deep immune profiling is essential for understanding the human immune system in health and disease. Successful biological interpretation of this data requires consistent laboratory processing with minimal batch-to-batch variation. Here, we detail a robust pipeline for the profiling of human...

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Bibliographic Details
Main Authors: Palak C. Genge, Charles R. Roll, Alexander T. Heubeck, Elliott Swanson, Nina Kondza, Cara Lord, Morgan Weiss, Veronica Hernandez, Cole Phalen, Zachary Thomson, Troy R. Torgerson, Peter J. Skene, Thomas F. Bumol, Julian Reading
Format: Article
Language:English
Published: Elsevier 2021-12-01
Series:STAR Protocols
Subjects:
Cell Biology
Single Cell
Flow Cytometry/Mass Cytometry
Sequencing
RNAseq
Immunology
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721006067
Description
Summary:Summary: Deep immune profiling is essential for understanding the human immune system in health and disease. Successful biological interpretation of this data requires consistent laboratory processing with minimal batch-to-batch variation. Here, we detail a robust pipeline for the profiling of human peripheral blood mononuclear cells by both high-dimensional flow cytometry and single-cell RNA-seq. These protocols reduce batch effects, generate reproducible data, and increase throughput.For complete details on the use and execution of this protocol, please refer to Savage et al. (2021).
ISSN:2666-1667

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