Optimized workflow for human PBMC multiomic immunosurveillance studies
Summary: Deep immune profiling is essential for understanding the human immune system in health and disease. Successful biological interpretation of this data requires consistent laboratory processing with minimal batch-to-batch variation. Here, we detail a robust pipeline for the profiling of human...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-12-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721006067 |
| _version_ | 1818873838753021952 |
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| author | Palak C. Genge Charles R. Roll Alexander T. Heubeck Elliott Swanson Nina Kondza Cara Lord Morgan Weiss Veronica Hernandez Cole Phalen Zachary Thomson Troy R. Torgerson Peter J. Skene Thomas F. Bumol Julian Reading |
| author_facet | Palak C. Genge Charles R. Roll Alexander T. Heubeck Elliott Swanson Nina Kondza Cara Lord Morgan Weiss Veronica Hernandez Cole Phalen Zachary Thomson Troy R. Torgerson Peter J. Skene Thomas F. Bumol Julian Reading |
| author_sort | Palak C. Genge |
| collection | DOAJ |
| description | Summary: Deep immune profiling is essential for understanding the human immune system in health and disease. Successful biological interpretation of this data requires consistent laboratory processing with minimal batch-to-batch variation. Here, we detail a robust pipeline for the profiling of human peripheral blood mononuclear cells by both high-dimensional flow cytometry and single-cell RNA-seq. These protocols reduce batch effects, generate reproducible data, and increase throughput.For complete details on the use and execution of this protocol, please refer to Savage et al. (2021). |
| first_indexed | 2024-12-19T13:01:04Z |
| format | Article |
| id | doaj.art-fae212fe174d4a2a85ae33c7cb9322d0 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-19T13:01:04Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-fae212fe174d4a2a85ae33c7cb9322d02022-12-21T20:20:15ZengElsevierSTAR Protocols2666-16672021-12-0124100900Optimized workflow for human PBMC multiomic immunosurveillance studiesPalak C. Genge0Charles R. Roll1Alexander T. Heubeck2Elliott Swanson3Nina Kondza4Cara Lord5Morgan Weiss6Veronica Hernandez7Cole Phalen8Zachary Thomson9Troy R. Torgerson10Peter J. Skene11Thomas F. Bumol12Julian Reading13Allen Institute for Immunology, 615 Westlake Avenue N, Seattle, WA 98109, USAAllen Institute for Immunology, 615 Westlake Avenue N, Seattle, WA 98109, USAAllen Institute for Immunology, 615 Westlake Avenue N, Seattle, WA 98109, USAAllen Institute for Immunology, 615 Westlake Avenue N, Seattle, WA 98109, USAAllen Institute for Immunology, 615 Westlake Avenue N, Seattle, WA 98109, USAAllen Institute for Immunology, 615 Westlake Avenue N, Seattle, WA 98109, USAAllen Institute for Immunology, 615 Westlake Avenue N, Seattle, WA 98109, USAAllen Institute for Immunology, 615 Westlake Avenue N, Seattle, WA 98109, USAAllen Institute for Immunology, 615 Westlake Avenue N, Seattle, WA 98109, USAAllen Institute for Immunology, 615 Westlake Avenue N, Seattle, WA 98109, USAAllen Institute for Immunology, 615 Westlake Avenue N, Seattle, WA 98109, USAAllen Institute for Immunology, 615 Westlake Avenue N, Seattle, WA 98109, USA; Corresponding authorAllen Institute for Immunology, 615 Westlake Avenue N, Seattle, WA 98109, USAAllen Institute for Immunology, 615 Westlake Avenue N, Seattle, WA 98109, USA; Corresponding authorSummary: Deep immune profiling is essential for understanding the human immune system in health and disease. Successful biological interpretation of this data requires consistent laboratory processing with minimal batch-to-batch variation. Here, we detail a robust pipeline for the profiling of human peripheral blood mononuclear cells by both high-dimensional flow cytometry and single-cell RNA-seq. These protocols reduce batch effects, generate reproducible data, and increase throughput.For complete details on the use and execution of this protocol, please refer to Savage et al. (2021).http://www.sciencedirect.com/science/article/pii/S2666166721006067Cell BiologySingle CellFlow Cytometry/Mass CytometrySequencingRNAseqImmunology |
| spellingShingle | Palak C. Genge Charles R. Roll Alexander T. Heubeck Elliott Swanson Nina Kondza Cara Lord Morgan Weiss Veronica Hernandez Cole Phalen Zachary Thomson Troy R. Torgerson Peter J. Skene Thomas F. Bumol Julian Reading Optimized workflow for human PBMC multiomic immunosurveillance studies STAR Protocols Cell Biology Single Cell Flow Cytometry/Mass Cytometry Sequencing RNAseq Immunology |
| title | Optimized workflow for human PBMC multiomic immunosurveillance studies |
| title_full | Optimized workflow for human PBMC multiomic immunosurveillance studies |
| title_fullStr | Optimized workflow for human PBMC multiomic immunosurveillance studies |
| title_full_unstemmed | Optimized workflow for human PBMC multiomic immunosurveillance studies |
| title_short | Optimized workflow for human PBMC multiomic immunosurveillance studies |
| title_sort | optimized workflow for human pbmc multiomic immunosurveillance studies |
| topic | Cell Biology Single Cell Flow Cytometry/Mass Cytometry Sequencing RNAseq Immunology |
| url | http://www.sciencedirect.com/science/article/pii/S2666166721006067 |
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