Impact of shipping temperature on cell viability and T cell responses to bacterial antigens [version 1; peer review: 2 approved]

Impact of shipping temperature on cell viability and T cell responses to bacterial antigens [version 1; peer review: 2 approved]

Background: Interferon-γ (IFN-γ) secretion by T cells is a key correlate of immune protection against many pathogens including tuberculosis and the neglected tropical disease melioidosis. Clinical studies in tropical regions of immune responses to pathogens and vaccine monitoring studies require the...

Πλήρης περιγραφή

Λεπτομέρειες βιβλιογραφικής εγγραφής
Κύριοι συγγραφείς:	Susanna J. Dunachie, Barbara Kronsteiner, Patpong Rongkard
Μορφή:	Άρθρο
Γλώσσα:	English
Έκδοση:	Wellcome 2023-04-01
Σειρά:	Wellcome Open Research
Θέματα:	IFN-gamma ELISpot PBMC cryopreservation shipping storage eng
Διαθέσιμο Online:	https://wellcomeopenresearch.org/articles/8-188/v1

Παρόμοια τεκμήρια

Optimal Thawing of Cryopreserved Peripheral Blood Mononuclear Cells for Use in High-Throughput Human Immune Monitoring Studies
ανά: Ramu A. Subbramanian, κ.ά.
Έκδοση: (2012-07-01)

A Positive Control for Detection of Functional CD4 T Cells in PBMC: The CPI Pool
ανά: Annemarie Schiller, κ.ά.
Έκδοση: (2017-12-01)

How much of Virus-Specific CD8 T Cell Reactivity is Detected with a Peptide Pool when Compared to Individual Peptides?
ανά: Ramu A. Subbramanian, κ.ά.
Έκδοση: (2012-10-01)

Improvement of IFNg ELISPOT Performance Following Overnight Resting of Frozen PBMC Samples Confirmed Through Rigorous Statistical Analysis
ανά: Radleigh Santos, κ.ά.
Έκδοση: (2014-12-01)

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ανά: Yan Wisnu Prajoko, κ.ά.
Έκδοση: (2024-02-01)

ELISPOT Refinement Using Spot Morphology for Assessing Host Responses to Tuberculosis
ανά: Sally A. Sharpe, κ.ά.
Έκδοση: (2012-03-01)

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ανά: Jennifer E. Snyder-Cappione, κ.ά.
Έκδοση: (2012-05-01)

Increased Level of IFN-γ and IL-4 Spot-Forming Cells on ELISPOT Assay as Biomarkers for Acute Graft-Versus-Host Disease and Concurrent Infections
ανά: Yoshihiro Komada, κ.ά.
Έκδοση: (2012-04-01)

NPM-ALK-reactive T-cell responses in children and adolescents with NPM-ALK positive anaplastic large cell lymphoma
ανά: Vijay Kumar Singh, κ.ά.
Έκδοση: (2019-09-01)

Comparative analysis of intradermal tuberculin test and γ-interferon assay for diagnosis of bovine tuberculosis
ανά: S S VYAVAHARE, κ.ά.
Έκδοση: (2022-09-01)

T Cell Responses against Orthopoxviruses in HIV-Positive Patients
ανά: Sammet Stefanie, κ.ά.
Έκδοση: (2024-01-01)

Identification of a First Human Norovirus CD8+ T Cell Epitope Restricted to HLA-A*0201 Allele
ανά: Maria Malm, κ.ά.
Έκδοση: (2018-11-01)

Interferon-γ: biological function and application for study of cellular immune response
ανά: A. A. Lutckii, κ.ά.
Έκδοση: (2016-02-01)

Interferon-gamma FlowSpot assay for the measurement of the T-cell response to cytomegalovirus
ανά: Qianyu Ye, κ.ά.
Έκδοση: (2023-06-01)

Optimizing Microneutralization and IFN-γ ELISPOT Assays to Evaluate Mpox Immunity
ανά: Yinyi Yu, κ.ά.
Έκδοση: (2024-12-01)

Validation of the Enzyme-Linked ImmunoSpot Analytic Method for the Detection of Human IFN-γ from Peripheral Blood Mononuclear Cells in Response to the SARS-CoV-2 Spike Protein
ανά: Laura E. Carreto-Binaghi, κ.ά.
Έκδοση: (2024-10-01)

Performance of International AIDS Vaccine Initiative African clinical research laboratories in standardised ELISpot and peripheral blood mononuclear cell processing in support of HIV vaccine clinical trials
ανά: Robert K. Langat, κ.ά.
Έκδοση: (2021-02-01)

Psychological well-being of travellers on long-distance shipping voyages: A systematic review protocol [version 2; peer review: 2 approved]
ανά: Nachieketa Khamari Sharma, κ.ά.
Έκδοση: (2024-11-01)

Harmonization and qualification of an IFN-γ Enzyme-Linked ImmunoSpot assay (ELISPOT) to measure influenza-specific cell-mediated immunity within the FLUCOP consortium
ανά: Gwenn Waerlop, κ.ά.
Έκδοση: (2022-09-01)

Impact of cryopreservation on CAR T production and clinical response
ανά: Karin Brezinger-Dayan, κ.ά.
Έκδοση: (2022-10-01)

Transient Increases in Inflammation and Proapoptotic Potential Are Associated with the HESN Phenotype Observed in a Subgroup of Kenyan Female Sex Workers
ανά: Marcel Gluchowski, κ.ά.
Έκδοση: (2022-02-01)

Low Avidity T Cells Do Not Hinder High Avidity T Cell Responses Against Melanoma
ανά: Kalliopi Ioannidou, κ.ά.
Έκδοση: (2019-09-01)

Pre-existing T-cell immunity to SARS-CoV-2 in unexposed healthy controls in Ecuador, as detected with a COVID-19 Interferon-Gamma Release Assay
ανά: Gustavo Echeverría, κ.ά.
Έκδοση: (2021-04-01)

Th1 and Th2 epitopes of Cowdria polymorphic gene 1 of Ehrlichia ruminantium
ανά: Tlou A. Ngoepe, κ.ά.
Έκδοση: (2023-03-01)

Longitudinal Analysis of Dengue Virus–Specific Memory T Cell Responses and Their Association With Clinical Outcome in Subsequent DENV Infection
ανά: Luis Alberto Sanchez-Vargas, κ.ά.
Έκδοση: (2021-07-01)

BpOmpW Antigen Stimulates the Necessary Protective T-Cell Responses Against Melioidosis
ανά: Julen Tomás-Cortázar, κ.ά.
Έκδοση: (2021-12-01)

An Enhanced ELISPOT Assay for Sensitive Detection of Antigen-Specific T Cell Responses to Borrelia burgdorferi
ανά: Gottfried H. Kellermann, κ.ά.
Έκδοση: (2013-09-01)

Digital twin enhanced agile design of ship pipeline systems [version 1; peer review: 1 approved, 3 approved with reservations]
ανά: Xin Wang, κ.ά.
Έκδοση: (2024-03-01)

Digital twin enhanced agile design of ship pipeline systems [version 2; peer review: 1 approved, 3 approved with reservations]
ανά: Xin Wang, κ.ά.
Έκδοση: (2025-01-01)

The Correlation between the Virus- and Brain Antigen-Specific B Cell Response in the Blood of Patients with Multiple Sclerosis
ανά: Marie Wunsch, κ.ά.
Έκδοση: (2016-04-01)

A high-throughput screening RT-qPCR assay for quantifying surrogate markers of immunity from PBMCs
ανά: Daniel J. Browne, κ.ά.
Έκδοση: (2022-08-01)

Immunoglobulin producing cells in chickens immunized with Eimeria tenella gametocyte antigen vaccines
ανά: M.M. Ayaz, κ.ά.
Έκδοση: (2008-04-01)

Comparison of the impact of allelic polymorphisms in PfAMA1 on the induction of T Cell responses in high and low malaria endemic communities in Ghana
ανά: Ebenezer A. Ofori, κ.ά.
Έκδοση: (2021-09-01)

T-cell activation of Toxoplasma gondii positive donors by maltodextrin nanoparticles formulated with killed Toxoplasma gondii
ανά: Monica Vargas-Montes, κ.ά.
Έκδοση: (2025-02-01)

Recombinant fusion protein of Heparin-Binding Hemagglutinin Adhesin and Fibronectin Attachment Protein (rHBHA-FAP) of Mycobacterium avium subsp. paratuberculosis elicits a strong gamma interferon response in peripheral blood mononuclear cell culture
ανά: Vida Eraghi, κ.ά.
Έκδοση: (2019-07-01)

ELISPOT Assay for Monitoring Cytotoxic T Lymphocytes (CTL) Activity in Cancer Vaccine Clinical Trials
ανά: Thomas J. Sayers, κ.ά.
Έκδοση: (2012-05-01)

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ανά: Mario Umberto Mondelli, κ.ά.
Έκδοση: (2012-11-01)

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ανά: Magdalena Tary-Lehmann, κ.ά.
Έκδοση: (2012-05-01)

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ανά: Flavio R. Sztajnbok, κ.ά.
Έκδοση: (2009-06-01)

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ανά: Laia Bosch-Camós, κ.ά.
Έκδοση: (2021-01-01)