Effects of degradable protein and non-fibre carbohydrates on microbial growth and fermentation in the rumen simulating fermenter (Rusitec)
A rumen simulation technique (Rusitec) apparatus with eight 800 ml fermentation vessels was used to investigate the effects of rumen degradable protein (RDP) level and non-fibre carbohydrate (NFC) type on ruminal fermentation, microbial growth, and populations of ruminal cellulolytic bacteria. Treat...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2015-05-01
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| Series: | Italian Journal of Animal Science |
| Subjects: | |
| Online Access: | http://www.aspajournal.it/index.php/ijas/article/view/3771 |
| _version_ | 1819035191433232384 |
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| author | Xiang H. Zhao Jian M. Gong Shan Zhou Chuan B. Fu Chan J. Liu Lan J. Xu Ke Pan Ming R. Qu |
| author_facet | Xiang H. Zhao Jian M. Gong Shan Zhou Chuan B. Fu Chan J. Liu Lan J. Xu Ke Pan Ming R. Qu |
| author_sort | Xiang H. Zhao |
| collection | DOAJ |
| description | A rumen simulation technique (Rusitec) apparatus with eight 800 ml fermentation vessels was used to investigate the effects of rumen degradable protein (RDP) level and non-fibre carbohydrate (NFC) type on ruminal fermentation, microbial growth, and populations of ruminal cellulolytic bacteria. Treatments consisted of two NFC types (starch and pectin) supplemented with 0 g/d (low RDP) or 1.56 g/d (high RDP) sodium caseinate. Apparent disappearance of dry matter and organic matter was greater for pectin than for starch treatment (P<0.01) with low or high RDP. A NFC × RDP interaction was observed for neutral detergent fibre disappearance (P=0.01), which was lower for pectin than for starch only under low RDP conditions. Compared with starch, pectin treatment increased the copy numbers of <em>Ruminococcus albus</em> (P≤0.01) and <em>Ruminococcus flavefaciens</em> (P≤0.09), the molar proportion of acetate (P<0.01), the acetate:propionate ratio (P<0.01), and methane production (P<0.01), but reduced the propionate proportion (P<0.01). Increasing dietary RDP increased the production of total VFA (P=0.01), methane (P<0.01), ammonia N (P<0.01), and microbial N (P<0.01). Significant NFC × RDP interaction and interaction tendency were observed for ammonia N production (P=0.01) and daily N flow of total microorganisms (P=0.07), which did not differ under low RDP conditions, but pectin produced greater microbial N and less ammonia N than starch with increased RDP. Results showed NFC type, RDP level, and their interaction affected ruminal fermentation and microbial growth, and under sufficient ruminal degradable N pectin had greater advantage in microbial N synthesis than starch <em>in vitro</em>. |
| first_indexed | 2024-12-21T07:45:42Z |
| format | Article |
| id | doaj.art-fae4cb5cd621402a9b28088cf788a8f6 |
| institution | Directory Open Access Journal |
| issn | 1594-4077 1828-051X |
| language | English |
| last_indexed | 2024-12-21T07:45:42Z |
| publishDate | 2015-05-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Italian Journal of Animal Science |
| spelling | doaj.art-fae4cb5cd621402a9b28088cf788a8f62022-12-21T19:11:13ZengTaylor & Francis GroupItalian Journal of Animal Science1594-40771828-051X2015-05-0114210.4081/ijas.2015.37712348Effects of degradable protein and non-fibre carbohydrates on microbial growth and fermentation in the rumen simulating fermenter (Rusitec)Xiang H. Zhao0Jian M. Gong1Shan Zhou2Chuan B. Fu3Chan J. Liu4Lan J. Xu5Ke Pan6Ming R. Qu7Jiangxi Province Key Laboratory of Animal Nutrition/Engineering Research Center of Feed Development, Jiangxi Agricultural University, NanchangJiangxi Province Key Laboratory of Animal Nutrition/Engineering Research Center of Feed Development, Jiangxi Agricultural University, NanchangJiangxi Province Key Laboratory of Animal Nutrition/Engineering Research Center of Feed Development, Jiangxi Agricultural University, NanchangJiangxi Province Key Laboratory of Animal Nutrition/Engineering Research Center of Feed Development, Jiangxi Agricultural University, NanchangJiangxi Province Key Laboratory of Animal Nutrition/Engineering Research Center of Feed Development, Jiangxi Agricultural University, NanchangJiangxi Province Key Laboratory of Animal Nutrition/Engineering Research Center of Feed Development, Jiangxi Agricultural University, NanchangJiangxi Province Key Laboratory of Animal Nutrition/Engineering Research Center of Feed Development, Jiangxi Agricultural University, NanchangJiangxi Province Key Laboratory of Animal Nutrition/Engineering Research Center of Feed Development, Jiangxi Agricultural University, NanchangA rumen simulation technique (Rusitec) apparatus with eight 800 ml fermentation vessels was used to investigate the effects of rumen degradable protein (RDP) level and non-fibre carbohydrate (NFC) type on ruminal fermentation, microbial growth, and populations of ruminal cellulolytic bacteria. Treatments consisted of two NFC types (starch and pectin) supplemented with 0 g/d (low RDP) or 1.56 g/d (high RDP) sodium caseinate. Apparent disappearance of dry matter and organic matter was greater for pectin than for starch treatment (P<0.01) with low or high RDP. A NFC × RDP interaction was observed for neutral detergent fibre disappearance (P=0.01), which was lower for pectin than for starch only under low RDP conditions. Compared with starch, pectin treatment increased the copy numbers of <em>Ruminococcus albus</em> (P≤0.01) and <em>Ruminococcus flavefaciens</em> (P≤0.09), the molar proportion of acetate (P<0.01), the acetate:propionate ratio (P<0.01), and methane production (P<0.01), but reduced the propionate proportion (P<0.01). Increasing dietary RDP increased the production of total VFA (P=0.01), methane (P<0.01), ammonia N (P<0.01), and microbial N (P<0.01). Significant NFC × RDP interaction and interaction tendency were observed for ammonia N production (P=0.01) and daily N flow of total microorganisms (P=0.07), which did not differ under low RDP conditions, but pectin produced greater microbial N and less ammonia N than starch with increased RDP. Results showed NFC type, RDP level, and their interaction affected ruminal fermentation and microbial growth, and under sufficient ruminal degradable N pectin had greater advantage in microbial N synthesis than starch <em>in vitro</em>.http://www.aspajournal.it/index.php/ijas/article/view/3771Microbial protein synthesisPectinRuminal degradable proteinRusitec |
| spellingShingle | Xiang H. Zhao Jian M. Gong Shan Zhou Chuan B. Fu Chan J. Liu Lan J. Xu Ke Pan Ming R. Qu Effects of degradable protein and non-fibre carbohydrates on microbial growth and fermentation in the rumen simulating fermenter (Rusitec) Italian Journal of Animal Science Microbial protein synthesis Pectin Ruminal degradable protein Rusitec |
| title | Effects of degradable protein and non-fibre carbohydrates on microbial growth and fermentation in the rumen simulating fermenter (Rusitec) |
| title_full | Effects of degradable protein and non-fibre carbohydrates on microbial growth and fermentation in the rumen simulating fermenter (Rusitec) |
| title_fullStr | Effects of degradable protein and non-fibre carbohydrates on microbial growth and fermentation in the rumen simulating fermenter (Rusitec) |
| title_full_unstemmed | Effects of degradable protein and non-fibre carbohydrates on microbial growth and fermentation in the rumen simulating fermenter (Rusitec) |
| title_short | Effects of degradable protein and non-fibre carbohydrates on microbial growth and fermentation in the rumen simulating fermenter (Rusitec) |
| title_sort | effects of degradable protein and non fibre carbohydrates on microbial growth and fermentation in the rumen simulating fermenter rusitec |
| topic | Microbial protein synthesis Pectin Ruminal degradable protein Rusitec |
| url | http://www.aspajournal.it/index.php/ijas/article/view/3771 |
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