Anti-sepsis effect of Xiaochaihu decoction based on the TLR4/MyD88/NF-κB signalling pathway
The aims of this study were to explore the protective effect of Xiaochaihu decoction in mice with sepsis induced by intraperitoneal injection; to explore its anti-inflammatory effect on the TLR4-MyD88-NF-κB signalling pathway; and to explore the main material basis of the anti-inflammatory effect of...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024027439 |
| _version_ | 1797259904407306240 |
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| author | Qingxin Yang Yulong Wang Gefei Cao Xiaoqing Li Tinghui Zhao |
| author_facet | Qingxin Yang Yulong Wang Gefei Cao Xiaoqing Li Tinghui Zhao |
| author_sort | Qingxin Yang |
| collection | DOAJ |
| description | The aims of this study were to explore the protective effect of Xiaochaihu decoction in mice with sepsis induced by intraperitoneal injection; to explore its anti-inflammatory effect on the TLR4-MyD88-NF-κB signalling pathway; and to explore the main material basis of the anti-inflammatory effect of Xiaochaihu decoction, with the aim of supplementing and expanding the associated research and providing a scientific foundation for the clinical use of the decoction. The effects of Xiaochaihu decoction on septic mice were analysed by measurements of white blood cells (WBC) and Platelets (PLT); Nitric Oxide (NO) level in serum; IL-6, IL-1β and TNF-α levels in serum; RT-PCR; Haematoxylin-Eosin (HE) immunohistochemistry; western blotting (WB). The results showed the excellent in vivo anti-inflammatory effects of Xiaochaihu decoction in LPS-induced septic mice, through down regulation of the gene and protein expression of TLR4, MYD88, TRAF6, IKK, IKBα and p65 and the subsequent reduction in the release of inflammatory mediators IL-6, IL-1β, TNF-α and NO. Moreover, significant anti-septic effect was observed from high and medium doses of Xiaochaihu decoction, but not from the low dose. |
| first_indexed | 2024-03-07T20:04:37Z |
| format | Article |
| id | doaj.art-fae4fee4472f45e083c8fdccb3586af2 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T23:16:50Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-fae4fee4472f45e083c8fdccb3586af22024-03-17T07:56:03ZengElsevierHeliyon2405-84402024-03-01105e26712Anti-sepsis effect of Xiaochaihu decoction based on the TLR4/MyD88/NF-κB signalling pathwayQingxin Yang0Yulong Wang1Gefei Cao2Xiaoqing Li3Tinghui Zhao4Department of Pharmacy, MianYang Orthopaedic Hospital, Mianyang, 621000, PR ChinaOffice of Party and Government Affairs, MianYang Orthopaedic Hospital, Mianyang, 621000, PR ChinaOffice of Party and Government Affairs, MianYang Orthopaedic Hospital, Mianyang, 621000, PR ChinaPharmaceutical Department, MianYang Hospital of Traditional Chinese Medicine, Mianyang, 621000, PR ChinaBurn and Plastic Surgery, MianYang Central Hospital, Mianyang, 621000, PR China; Corresponding author.The aims of this study were to explore the protective effect of Xiaochaihu decoction in mice with sepsis induced by intraperitoneal injection; to explore its anti-inflammatory effect on the TLR4-MyD88-NF-κB signalling pathway; and to explore the main material basis of the anti-inflammatory effect of Xiaochaihu decoction, with the aim of supplementing and expanding the associated research and providing a scientific foundation for the clinical use of the decoction. The effects of Xiaochaihu decoction on septic mice were analysed by measurements of white blood cells (WBC) and Platelets (PLT); Nitric Oxide (NO) level in serum; IL-6, IL-1β and TNF-α levels in serum; RT-PCR; Haematoxylin-Eosin (HE) immunohistochemistry; western blotting (WB). The results showed the excellent in vivo anti-inflammatory effects of Xiaochaihu decoction in LPS-induced septic mice, through down regulation of the gene and protein expression of TLR4, MYD88, TRAF6, IKK, IKBα and p65 and the subsequent reduction in the release of inflammatory mediators IL-6, IL-1β, TNF-α and NO. Moreover, significant anti-septic effect was observed from high and medium doses of Xiaochaihu decoction, but not from the low dose.http://www.sciencedirect.com/science/article/pii/S2405844024027439SepsisXiaochaihu decoctionTLR4MyD88NF-κBLPS |
| spellingShingle | Qingxin Yang Yulong Wang Gefei Cao Xiaoqing Li Tinghui Zhao Anti-sepsis effect of Xiaochaihu decoction based on the TLR4/MyD88/NF-κB signalling pathway Heliyon Sepsis Xiaochaihu decoction TLR4 MyD88 NF-κB LPS |
| title | Anti-sepsis effect of Xiaochaihu decoction based on the TLR4/MyD88/NF-κB signalling pathway |
| title_full | Anti-sepsis effect of Xiaochaihu decoction based on the TLR4/MyD88/NF-κB signalling pathway |
| title_fullStr | Anti-sepsis effect of Xiaochaihu decoction based on the TLR4/MyD88/NF-κB signalling pathway |
| title_full_unstemmed | Anti-sepsis effect of Xiaochaihu decoction based on the TLR4/MyD88/NF-κB signalling pathway |
| title_short | Anti-sepsis effect of Xiaochaihu decoction based on the TLR4/MyD88/NF-κB signalling pathway |
| title_sort | anti sepsis effect of xiaochaihu decoction based on the tlr4 myd88 nf κb signalling pathway |
| topic | Sepsis Xiaochaihu decoction TLR4 MyD88 NF-κB LPS |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024027439 |
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