Identification of S alleles associated with self-incompatibility in capuli (<em>Prunus serótina</em> subsp. <em>capulí</em>) samples by amplification of the Intron I of the S-RNasa gene
<p>In plants, gametophytic self-incompatibility is a genetic mechanism regulated by the S locus, which has evolved to prevent self-fertilization. In fruit crops, information regarding the allelic composition of the S locus is essential for the establishment of productive orchards, as this alle...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Universidad San Francisco de Quito USFQ
2015-05-01
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| Series: | ACI Avances en Ciencias e Ingenierías |
| Subjects: | |
| Online Access: | http://revistas.usfq.edu.ec/index.php/avances/article/view/224 |
| Summary: | <p>In plants, gametophytic self-incompatibility is a genetic mechanism regulated by the S locus, which has evolved to prevent self-fertilization. In fruit crops, information regarding the allelic composition of the S locus is essential for the establishment of productive orchards, as this allelic composition defines compatible combinations between individuals. The identification and cloning of S-RNase genes in <em>Prunus</em> species has allowed the development of molecular techniques for the characterization of S genotypes in wild and less-studied species of the genus. In this study we evaluated 80 individuals of capulí (<em>Prunus serotina</em> subsp. <em>capulí</em>) collected from 8 provinces of the Ecuadorian highlands to determinate the degree of allelic diversity of the S locus in this species. The molecular characterization of S loci was performed using degenerate primers designed from conserved regions of the S-RNase gene of several <em>Prunus</em> species. PCR products were separated on agarose gels, classified based on band size and sequenced. Our results reveal the presence of 11 alleles across sampled individuals. Generally, identified alleles showed a high percentage of identity with S-locus sequences reported for other species of the genus and it can be speculated that these derive from a common ancestor. By contrast, sequences with a lower percentage of identity may have originated independently following the diversification of <em>Prunus</em> species. The results obtained in this study should be complemented with field tests to confirm the phenotypic behavior of the capuli individuals analyzed.</p> |
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| ISSN: | 1390-5384 2528-7788 |