Microfluidic-Based Droplets for Advanced Regenerative Medicine: Current Challenges and Future Trends
Microfluidics is a promising approach for the facile and large-scale fabrication of monodispersed droplets for various applications in biomedicine. This technology has demonstrated great potential to address the limitations of regenerative medicine. Microfluidics provides safe, accurate, reliable, a...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/12/1/20 |
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| author | Hojjatollah Nazari Asieh Heirani-Tabasi Sadegh Ghorbani Hossein Eyni Sajad Razavi Bazaz Maryam Khayati Fatemeh Gheidari Keyvan Moradpour Mousa Kehtari Seyed Mohsen Ahmadi Tafti Seyed Hossein Ahmadi Tafti Majid Ebrahimi Warkiani |
| author_facet | Hojjatollah Nazari Asieh Heirani-Tabasi Sadegh Ghorbani Hossein Eyni Sajad Razavi Bazaz Maryam Khayati Fatemeh Gheidari Keyvan Moradpour Mousa Kehtari Seyed Mohsen Ahmadi Tafti Seyed Hossein Ahmadi Tafti Majid Ebrahimi Warkiani |
| author_sort | Hojjatollah Nazari |
| collection | DOAJ |
| description | Microfluidics is a promising approach for the facile and large-scale fabrication of monodispersed droplets for various applications in biomedicine. This technology has demonstrated great potential to address the limitations of regenerative medicine. Microfluidics provides safe, accurate, reliable, and cost-effective methods for encapsulating different stem cells, gametes, biomaterials, biomolecules, reagents, genes, and nanoparticles inside picoliter-sized droplets or droplet-derived microgels for different applications. Moreover, microenvironments made using such droplets can mimic niches of stem cells for cell therapy purposes, simulate native extracellular matrix (ECM) for tissue engineering applications, and remove challenges in cell encapsulation and three-dimensional (3D) culture methods. The fabrication of droplets using microfluidics also provides controllable microenvironments for manipulating gametes, fertilization, and embryo cultures for reproductive medicine. This review focuses on the relevant studies, and the latest progress in applying droplets in stem cell therapy, tissue engineering, reproductive biology, and gene therapy are separately evaluated. In the end, we discuss the challenges ahead in the field of microfluidics-based droplets for advanced regenerative medicine. |
| first_indexed | 2024-03-10T01:49:38Z |
| format | Article |
| id | doaj.art-fae6accc65a24afba510b7bea66f6c4e |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T01:49:38Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-fae6accc65a24afba510b7bea66f6c4e2023-11-23T13:08:04ZengMDPI AGBiosensors2079-63742021-12-011212010.3390/bios12010020Microfluidic-Based Droplets for Advanced Regenerative Medicine: Current Challenges and Future TrendsHojjatollah Nazari0Asieh Heirani-Tabasi1Sadegh Ghorbani2Hossein Eyni3Sajad Razavi Bazaz4Maryam Khayati5Fatemeh Gheidari6Keyvan Moradpour7Mousa Kehtari8Seyed Mohsen Ahmadi Tafti9Seyed Hossein Ahmadi Tafti10Majid Ebrahimi Warkiani11School of Biomedical Engineering, University of Technology Sydney, Sydney, NSW 2007, AustraliaResearch Center for Advanced Technologies in Cardiovascular Medicine, Tehran Heart Center Hospital, Tehran University of Medical Sciences, Tehran 14535, IranInterdisciplinary Nanoscience Center (iNANO), Aarhus University, 8000 Aarhus, DenmarkCellular and Molecular Research Center, School of Medicine, Iran University of Medical Sciences, Tehran 14535, IranSchool of Biomedical Engineering, University of Technology Sydney, Sydney, NSW 2007, AustraliaDepartment of Pharmaceutical Nanotechnology, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan 45371, IranDepartment of Biotechnology, University of Tehran, Tehran 14535, IranDepartment of Chemical Engineering, Sharif University of Technology, Tehran 14535, IranDepartment of Biology, Faculty of Science, University of Tehran, Tehran 14535, IranColorectal Surgery Research Center, Imam Hospital Complex, Tehran University of Medical Sciences, Tehran 14535, IranResearch Center for Advanced Technologies in Cardiovascular Medicine, Tehran Heart Center Hospital, Tehran University of Medical Sciences, Tehran 14535, IranSchool of Biomedical Engineering, University of Technology Sydney, Sydney, NSW 2007, AustraliaMicrofluidics is a promising approach for the facile and large-scale fabrication of monodispersed droplets for various applications in biomedicine. This technology has demonstrated great potential to address the limitations of regenerative medicine. Microfluidics provides safe, accurate, reliable, and cost-effective methods for encapsulating different stem cells, gametes, biomaterials, biomolecules, reagents, genes, and nanoparticles inside picoliter-sized droplets or droplet-derived microgels for different applications. Moreover, microenvironments made using such droplets can mimic niches of stem cells for cell therapy purposes, simulate native extracellular matrix (ECM) for tissue engineering applications, and remove challenges in cell encapsulation and three-dimensional (3D) culture methods. The fabrication of droplets using microfluidics also provides controllable microenvironments for manipulating gametes, fertilization, and embryo cultures for reproductive medicine. This review focuses on the relevant studies, and the latest progress in applying droplets in stem cell therapy, tissue engineering, reproductive biology, and gene therapy are separately evaluated. In the end, we discuss the challenges ahead in the field of microfluidics-based droplets for advanced regenerative medicine.https://www.mdpi.com/2079-6374/12/1/20dropletmicrofluidicsregenerative medicinestem cell therapytissue engineering |
| spellingShingle | Hojjatollah Nazari Asieh Heirani-Tabasi Sadegh Ghorbani Hossein Eyni Sajad Razavi Bazaz Maryam Khayati Fatemeh Gheidari Keyvan Moradpour Mousa Kehtari Seyed Mohsen Ahmadi Tafti Seyed Hossein Ahmadi Tafti Majid Ebrahimi Warkiani Microfluidic-Based Droplets for Advanced Regenerative Medicine: Current Challenges and Future Trends Biosensors droplet microfluidics regenerative medicine stem cell therapy tissue engineering |
| title | Microfluidic-Based Droplets for Advanced Regenerative Medicine: Current Challenges and Future Trends |
| title_full | Microfluidic-Based Droplets for Advanced Regenerative Medicine: Current Challenges and Future Trends |
| title_fullStr | Microfluidic-Based Droplets for Advanced Regenerative Medicine: Current Challenges and Future Trends |
| title_full_unstemmed | Microfluidic-Based Droplets for Advanced Regenerative Medicine: Current Challenges and Future Trends |
| title_short | Microfluidic-Based Droplets for Advanced Regenerative Medicine: Current Challenges and Future Trends |
| title_sort | microfluidic based droplets for advanced regenerative medicine current challenges and future trends |
| topic | droplet microfluidics regenerative medicine stem cell therapy tissue engineering |
| url | https://www.mdpi.com/2079-6374/12/1/20 |
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