Evaluation of the Hepato and Nephron-Protective Effect of a Polyherbal Mixture using Wistar Albino Rats
Aim: A polyherbal formulation prepared from a mixture of leaves of Gongronema latifolia, Ocimum gratissimum and Vernonia amygdalina (GOV) was evaluated for hepato-nephro protective properties against acetaminophen-induced toxicity in Wistar albino rats. Materials and Methods: Normal Wistar albi...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2014-06-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/4501/5875_PFA(AK)_PF2(AGAK)_PFA2(AK)_PF2(PAG)_PF2(PN).pdf |
| Summary: | Aim: A polyherbal formulation prepared from a mixture of leaves
of Gongronema latifolia, Ocimum gratissimum and Vernonia
amygdalina (GOV) was evaluated for hepato-nephro protective
properties against acetaminophen-induced toxicity in Wistar
albino rats.
Materials and Methods: Normal Wistar albino rats were orally
treated with different doses of GOV extract (2, 4 and 8 g/kg b.
wt), distilled water and some standard hepatoprotective drugs
such as Liv 52 and silymarin for 14 days. However, a day prior to
the 14th day, 3 g/kg body weight dose of Acetaminophen (APAP)
was administered p.o. 1h before GOV and the standard drugs to
induce hepatic and renal damage. The normal control was setup
which received only distilled water. The serum levels of liver
marker enzymes, biochemical analytes, antioxidant enzymes and
hematological parameters were monitored.
Results: The results showed that pretreatment of experimental
animals with a different doses of the polyherbal formulation
dose dependently caused a significant (p≤0.05) increase in the
levels of most of the measured hematological parameters but
significantly (p≤0.05) reduced the levels of MCV and monocytes
when compared to the APAP induced toxin control group. Rats
pretreated with GOV exhibited significant (p< 0.05) increase
in serum levels of ALP, ALT, AST, GGT, LDH, Cholesterol,
Triglycerides, Urea and a subsequent decrease in Albumin,
Creatine and Total protein when compared to the normal rats.
This trend in enzyme and biochemical analytes levels were
significantly (p< 0.05) reversed when compared to toxin control
group. GOV significantly (p< 0.05) and dose dependently
increased the serum, kidney and hepatic CAT, GPx, GSH, GST,
SOD and total protein activity in APAP induced damage in rats
compared to the toxin control groups.
Conclusion: The data from this study suggest that the polyherbal
formulation possess hepato and nephron-protective potential
against acetaminophen induced hepatotoxicity in rats, thus
providing scientific rationale for its use in traditional medicine for
the treatment of liver diseases. |
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| ISSN: | 2249-782X 0973-709X |