GC-MS Based Characterization, Antibacterial, Antifungal and Anti-Oncogenic Activity of Ethyl Acetate Extract of <i>Aspergillus niger</i> Strain AK-6 Isolated from Rhizospheric Soil

Rhizospheric soil is the richest niche of different microbes that produce biologically active metabolites. The current study investigated the antimicrobial, antifungal and anticancer activities of ethyl acetate extract of the potent rhizospheric fungus <i>Aspergillus niger</i> AK6 (AK-6)...

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Main Authors: Shaik Kalimulla Niazi, Dhanyakumara Shivapoojar Basavarajappa, Sushma Hatti Kumaraswamy, Asmatanzeem Bepari, Halaswamy Hiremath, Shashiraj Kariyellappa Nagaraja, Muthuraj Rudrappa, Anil Hugar, Mary Anne Wong Cordero, Sreenivasa Nayaka
Format: Article
Language:English
Published: MDPI AG 2023-04-01
Series:Current Issues in Molecular Biology
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Online Access:https://www.mdpi.com/1467-3045/45/5/241
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Summary:Rhizospheric soil is the richest niche of different microbes that produce biologically active metabolites. The current study investigated the antimicrobial, antifungal and anticancer activities of ethyl acetate extract of the potent rhizospheric fungus <i>Aspergillus niger</i> AK6 (AK-6). A total of six fungal isolates were isolated, and isolate AK-6 was selected based on primary screening. Further, it exhibited moderate antimicrobial activity against pathogens such as <i>Klebsiella pneumonia</i>, <i>Candida albicans</i>, <i>Escherichia coli</i>, <i>Shigella flexneri</i>, <i>Bacillus subtilis</i> and <i>Staphylococcus aureus</i>. The morphological and molecular characterization (18S rRNA) confirmed that the isolate AK-6 belonged to Aspergillus niger. Further, AK-6 showed potent antifungal activity with 47.2%, 59.4% and 64.1% of inhibition against <i>Sclerotium rolfsii</i>, <i>Cercospora canescens</i> and <i>Fusarium sambucinum</i> phytopathogens. FT-IR analysis displayed different biological functional groups. Consequently, the GC-MS analysis displayed bioactive compounds, namely, n-didehydrohexacarboxyl-2,4,5-trimethylpiperazine (23.82%), dibutyl phthalate (14.65%), e-5-heptadecanol (8.98%), and 2,4-ditert-butylphenol (8.60%), among the total of 15 compounds isolated. Further, the anticancer activity of AK-6 was exhibited against the MCF-7 cell line of human breast adenocarcinoma with an IC<sub>50</sub> value of 102.01 μg/mL. Furthermore, flow cytometry depicted 17.3%, 26.43%, and 3.16% of early and late apoptosis and necrosis in the AK-6 extarct treated MCF-7 cell line, respectively. The results of the present analysis suggest that the isolated <i>Aspergillus niger</i> strain AK-6 extract has the potential to be explored as a promising antimicrobial, antifungal and anticancer drug for medical and agricultural applications.
ISSN:1467-3037
1467-3045