Gene delivery to pancreatic exocrine cells <it>in vivo</it> and <it>in vitro</it>
<p>Abstract</p> <p>Background</p> <p>Effective gene transfer to the pancreas or to pancreatic cells has remained elusive although it is essential for studies of genetic lineage tracing and modulation of gene expression. Different transduction methods and viral vectors w...
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| Format: | Article |
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BMC
2012-10-01
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| Series: | BMC Biotechnology |
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| Online Access: | http://www.biomedcentral.com/1472-6750/12/74 |
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| author | Houbracken Isabelle Baeyens Luc Ravassard Philippe Heimberg Harry Bouwens Luc |
| author_facet | Houbracken Isabelle Baeyens Luc Ravassard Philippe Heimberg Harry Bouwens Luc |
| author_sort | Houbracken Isabelle |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Effective gene transfer to the pancreas or to pancreatic cells has remained elusive although it is essential for studies of genetic lineage tracing and modulation of gene expression. Different transduction methods and viral vectors were tested in vitro and in vivo, in rat and mouse pancreas.</p> <p>Results</p> <p>For in vitro transfection/transduction of rat exocrine cells lipofection reagents, adenoviral vectors, and Mokola- and VSV-G pseudotyped lentiviral vectors were used. For in vivo transduction of mouse and rat pancreas adenoviral vectors and VSV-G lentiviral vectors were injected into the parenchymal tissue. Both lipofection of rat exocrine cell cultures and transduction with Mokola pseudotyped lentiviral vectors were inefficient and resulted in less than 4% EGFP expressing cells. Adenoviral transduction was highly efficient but its usefulness for gene delivery to rat exocrine cells in vitro was hampered by a drastic increase in cell death. In vitro transduction of rat exocrine cells was most optimal with VSV-G pseudotyped lentiviral vectors, with stable transgene expression, no significant effect on cell survival and about 40% transduced cells. In vivo, pancreatic cells could not be transduced by intra-parenchymal administration of lentiviral vectors in mouse and rat pancreas. However, a high efficiency could be obtained by adenoviral vectors, resulting in transient transduction of mainly exocrine acinar cells. Injection in immune-deficient animals diminished leukocyte infiltration and prolonged transgene expression.</p> <p>Conclusions</p> <p>In summary, our study remarkably demonstrates that transduction of pancreatic exocrine cells requires lentiviral vectors in vitro but adenoviral vectors in vivo.</p> |
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| id | doaj.art-faecd034ee244e688a79ac2750198aad |
| institution | Directory Open Access Journal |
| issn | 1472-6750 |
| language | English |
| last_indexed | 2024-12-13T16:49:10Z |
| publishDate | 2012-10-01 |
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| series | BMC Biotechnology |
| spelling | doaj.art-faecd034ee244e688a79ac2750198aad2022-12-21T23:38:05ZengBMCBMC Biotechnology1472-67502012-10-011217410.1186/1472-6750-12-74Gene delivery to pancreatic exocrine cells <it>in vivo</it> and <it>in vitro</it>Houbracken IsabelleBaeyens LucRavassard PhilippeHeimberg HarryBouwens Luc<p>Abstract</p> <p>Background</p> <p>Effective gene transfer to the pancreas or to pancreatic cells has remained elusive although it is essential for studies of genetic lineage tracing and modulation of gene expression. Different transduction methods and viral vectors were tested in vitro and in vivo, in rat and mouse pancreas.</p> <p>Results</p> <p>For in vitro transfection/transduction of rat exocrine cells lipofection reagents, adenoviral vectors, and Mokola- and VSV-G pseudotyped lentiviral vectors were used. For in vivo transduction of mouse and rat pancreas adenoviral vectors and VSV-G lentiviral vectors were injected into the parenchymal tissue. Both lipofection of rat exocrine cell cultures and transduction with Mokola pseudotyped lentiviral vectors were inefficient and resulted in less than 4% EGFP expressing cells. Adenoviral transduction was highly efficient but its usefulness for gene delivery to rat exocrine cells in vitro was hampered by a drastic increase in cell death. In vitro transduction of rat exocrine cells was most optimal with VSV-G pseudotyped lentiviral vectors, with stable transgene expression, no significant effect on cell survival and about 40% transduced cells. In vivo, pancreatic cells could not be transduced by intra-parenchymal administration of lentiviral vectors in mouse and rat pancreas. However, a high efficiency could be obtained by adenoviral vectors, resulting in transient transduction of mainly exocrine acinar cells. Injection in immune-deficient animals diminished leukocyte infiltration and prolonged transgene expression.</p> <p>Conclusions</p> <p>In summary, our study remarkably demonstrates that transduction of pancreatic exocrine cells requires lentiviral vectors in vitro but adenoviral vectors in vivo.</p>http://www.biomedcentral.com/1472-6750/12/74Lentiviral vectorAdenoviral vectorLipofectionGene transferPancreasAcinar cell |
| spellingShingle | Houbracken Isabelle Baeyens Luc Ravassard Philippe Heimberg Harry Bouwens Luc Gene delivery to pancreatic exocrine cells <it>in vivo</it> and <it>in vitro</it> BMC Biotechnology Lentiviral vector Adenoviral vector Lipofection Gene transfer Pancreas Acinar cell |
| title | Gene delivery to pancreatic exocrine cells <it>in vivo</it> and <it>in vitro</it> |
| title_full | Gene delivery to pancreatic exocrine cells <it>in vivo</it> and <it>in vitro</it> |
| title_fullStr | Gene delivery to pancreatic exocrine cells <it>in vivo</it> and <it>in vitro</it> |
| title_full_unstemmed | Gene delivery to pancreatic exocrine cells <it>in vivo</it> and <it>in vitro</it> |
| title_short | Gene delivery to pancreatic exocrine cells <it>in vivo</it> and <it>in vitro</it> |
| title_sort | gene delivery to pancreatic exocrine cells it in vivo it and it in vitro it |
| topic | Lentiviral vector Adenoviral vector Lipofection Gene transfer Pancreas Acinar cell |
| url | http://www.biomedcentral.com/1472-6750/12/74 |
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