CSDR Coupling with Exo III for Ultrasensitive Electrochemistry Determination of miR-145
Recently, miRNAs have become a promising biomarker for disease diagnostics. miRNA-145 is closely related to strokes. The accuracy determination of miRNA-145 (miR-145) in stroke patients still remains challenging due to its heterogeneity and low abundance, as well as the complexity of the blood matri...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/5/2208 |
| _version_ | 1827752492472991744 |
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| author | Moli Zhang Yang Yang Lingyi Xin Hua Zhang Lun Wu Jun Zhu Jing Zhu Shiyun Liu Zhaohui Wang Qinhua Chen Guangyi Yang |
| author_facet | Moli Zhang Yang Yang Lingyi Xin Hua Zhang Lun Wu Jun Zhu Jing Zhu Shiyun Liu Zhaohui Wang Qinhua Chen Guangyi Yang |
| author_sort | Moli Zhang |
| collection | DOAJ |
| description | Recently, miRNAs have become a promising biomarker for disease diagnostics. miRNA-145 is closely related to strokes. The accuracy determination of miRNA-145 (miR-145) in stroke patients still remains challenging due to its heterogeneity and low abundance, as well as the complexity of the blood matrix. In this work, we developed a novel electrochemical miRNA-145 biosensor via subtly coupling the cascade strand displacement reaction (CSDR), exonuclease III (Exo III), and magnetic nanoparticles (MNPs). The developed electrochemical biosensor can quantitatively detect miRNA-145 ranging from 1 × 10<sup>2</sup> to 1 × 10<sup>6</sup> aM with a detection limit as low down as 100 aM. This biosensor also exhibits excellent specificity to distinguish similar miRNA sequences even with single-base differences. It has been successfully applied to distinguish healthy people from stroke patients. The results of this biosensor are consistent with the results of the reverse transcription quantitative polymerase chain reaction (RT-qPCR). The proposed electrochemical biosensor has great potential applications for biomedical research on and clinical diagnosis of strokes. |
| first_indexed | 2024-03-11T07:17:10Z |
| format | Article |
| id | doaj.art-faecd4aab70b44f5af3a95857ef6f1dc |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T07:17:10Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-faecd4aab70b44f5af3a95857ef6f1dc2023-11-17T08:13:31ZengMDPI AGMolecules1420-30492023-02-01285220810.3390/molecules28052208CSDR Coupling with Exo III for Ultrasensitive Electrochemistry Determination of miR-145Moli Zhang0Yang Yang1Lingyi Xin2Hua Zhang3Lun Wu4Jun Zhu5Jing Zhu6Shiyun Liu7Zhaohui Wang8Qinhua Chen9Guangyi Yang10Shenzhen Bao’an Authentic TCM Therapy Hospital, Shenzhen 518102, ChinaShenzhen Bao’an Authentic TCM Therapy Hospital, Shenzhen 518102, ChinaShenzhen Bao’an Traditional Chinese Medicine Hospital, Guangzhou University of Chinese Medicine, Shenzhen 518000, ChinaSinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan 430345, ChinaSinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan 430345, ChinaSinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan 430345, ChinaShenzhen Bao’an Traditional Chinese Medicine Hospital, Guangzhou University of Chinese Medicine, Shenzhen 518000, ChinaSinopharm Dongfeng General Hospital, Hubei University of Medicine, Shiyan 430345, ChinaShenzhen Bao’an Authentic TCM Therapy Hospital, Shenzhen 518102, ChinaShenzhen Bao’an Authentic TCM Therapy Hospital, Shenzhen 518102, ChinaShenzhen Bao’an Traditional Chinese Medicine Hospital, Guangzhou University of Chinese Medicine, Shenzhen 518000, ChinaRecently, miRNAs have become a promising biomarker for disease diagnostics. miRNA-145 is closely related to strokes. The accuracy determination of miRNA-145 (miR-145) in stroke patients still remains challenging due to its heterogeneity and low abundance, as well as the complexity of the blood matrix. In this work, we developed a novel electrochemical miRNA-145 biosensor via subtly coupling the cascade strand displacement reaction (CSDR), exonuclease III (Exo III), and magnetic nanoparticles (MNPs). The developed electrochemical biosensor can quantitatively detect miRNA-145 ranging from 1 × 10<sup>2</sup> to 1 × 10<sup>6</sup> aM with a detection limit as low down as 100 aM. This biosensor also exhibits excellent specificity to distinguish similar miRNA sequences even with single-base differences. It has been successfully applied to distinguish healthy people from stroke patients. The results of this biosensor are consistent with the results of the reverse transcription quantitative polymerase chain reaction (RT-qPCR). The proposed electrochemical biosensor has great potential applications for biomedical research on and clinical diagnosis of strokes.https://www.mdpi.com/1420-3049/28/5/2208electrochemistrymiRNA-145strokeCSDRExo IIIMNPs |
| spellingShingle | Moli Zhang Yang Yang Lingyi Xin Hua Zhang Lun Wu Jun Zhu Jing Zhu Shiyun Liu Zhaohui Wang Qinhua Chen Guangyi Yang CSDR Coupling with Exo III for Ultrasensitive Electrochemistry Determination of miR-145 Molecules electrochemistry miRNA-145 stroke CSDR Exo III MNPs |
| title | CSDR Coupling with Exo III for Ultrasensitive Electrochemistry Determination of miR-145 |
| title_full | CSDR Coupling with Exo III for Ultrasensitive Electrochemistry Determination of miR-145 |
| title_fullStr | CSDR Coupling with Exo III for Ultrasensitive Electrochemistry Determination of miR-145 |
| title_full_unstemmed | CSDR Coupling with Exo III for Ultrasensitive Electrochemistry Determination of miR-145 |
| title_short | CSDR Coupling with Exo III for Ultrasensitive Electrochemistry Determination of miR-145 |
| title_sort | csdr coupling with exo iii for ultrasensitive electrochemistry determination of mir 145 |
| topic | electrochemistry miRNA-145 stroke CSDR Exo III MNPs |
| url | https://www.mdpi.com/1420-3049/28/5/2208 |
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