Protocol to analyze the inhibitory effect of free organic carbon generated by chemoautotrophic bacteria on their CO2 fixation
Summary: Here, we present a protocol to analyze the inhibition of self-generated extracellular free organic carbon (EFOC) on CO2 fixation by chemoautotrophic bacteria. We detail the construction and operation of membrane reactor, followed by a simulation experiment to verify the inhibition of EFOC o...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S266616672300254X |
| _version_ | 1797832331481841664 |
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| author | Saiwei Zhang Xiaohua Fu Ya-nan Wang Lei Wang |
| author_facet | Saiwei Zhang Xiaohua Fu Ya-nan Wang Lei Wang |
| author_sort | Saiwei Zhang |
| collection | DOAJ |
| description | Summary: Here, we present a protocol to analyze the inhibition of self-generated extracellular free organic carbon (EFOC) on CO2 fixation by chemoautotrophic bacteria. We detail the construction and operation of membrane reactor, followed by a simulation experiment to verify the inhibition of EFOC on CO2 fixation. We further describe the analysis of main inhibitory components in EFOC and measurement of abundance and transcription level of ribulose bisphosphate carboxylase/oxygenase (RuBisCO) gene to clarify the mechanism of the main inhibition components on CO2 fixation.For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| first_indexed | 2024-04-09T14:06:06Z |
| format | Article |
| id | doaj.art-faee7bc1a2844b22b4ed4ba2cfb34c28 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-04-09T14:06:06Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-faee7bc1a2844b22b4ed4ba2cfb34c282023-05-07T04:17:42ZengElsevierSTAR Protocols2666-16672023-06-0142102287Protocol to analyze the inhibitory effect of free organic carbon generated by chemoautotrophic bacteria on their CO2 fixationSaiwei Zhang0Xiaohua Fu1Ya-nan Wang2Lei Wang3Shanghai Academy of Environmental Sciences, Shanghai 200233, China; State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, ChinaState Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, ChinaQingdao Solid Waste Pollution Control and Resource Engineering Research Center, College of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao 266033, ChinaState Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China; Corresponding authorSummary: Here, we present a protocol to analyze the inhibition of self-generated extracellular free organic carbon (EFOC) on CO2 fixation by chemoautotrophic bacteria. We detail the construction and operation of membrane reactor, followed by a simulation experiment to verify the inhibition of EFOC on CO2 fixation. We further describe the analysis of main inhibitory components in EFOC and measurement of abundance and transcription level of ribulose bisphosphate carboxylase/oxygenase (RuBisCO) gene to clarify the mechanism of the main inhibition components on CO2 fixation.For complete details on the use and execution of this protocol, please refer to Zhang et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S266616672300254XMicrobiologyMolecular BiologyGene ExpressionMass SpectrometryBiotechnology and bioengineeringEnvironmental sciences |
| spellingShingle | Saiwei Zhang Xiaohua Fu Ya-nan Wang Lei Wang Protocol to analyze the inhibitory effect of free organic carbon generated by chemoautotrophic bacteria on their CO2 fixation STAR Protocols Microbiology Molecular Biology Gene Expression Mass Spectrometry Biotechnology and bioengineering Environmental sciences |
| title | Protocol to analyze the inhibitory effect of free organic carbon generated by chemoautotrophic bacteria on their CO2 fixation |
| title_full | Protocol to analyze the inhibitory effect of free organic carbon generated by chemoautotrophic bacteria on their CO2 fixation |
| title_fullStr | Protocol to analyze the inhibitory effect of free organic carbon generated by chemoautotrophic bacteria on their CO2 fixation |
| title_full_unstemmed | Protocol to analyze the inhibitory effect of free organic carbon generated by chemoautotrophic bacteria on their CO2 fixation |
| title_short | Protocol to analyze the inhibitory effect of free organic carbon generated by chemoautotrophic bacteria on their CO2 fixation |
| title_sort | protocol to analyze the inhibitory effect of free organic carbon generated by chemoautotrophic bacteria on their co2 fixation |
| topic | Microbiology Molecular Biology Gene Expression Mass Spectrometry Biotechnology and bioengineering Environmental sciences |
| url | http://www.sciencedirect.com/science/article/pii/S266616672300254X |
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