NADES Compounds Identified in <i>Hypoxis hemerocallidea</i> Corms during Dormancy
Soaking <i>Hypoxis hemerocallidea</i> corms in distilled water improved the propagation and development of cormlets, suggesting the potential leaching-out of inhibitory chemical compounds. To investigate the presence of inhibitory compounds, nuclear magnetic resonance (NMR) spectral data...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/18/2387 |
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| author | Motiki M. Mofokeng Gerhard Prinsloo Hintsa T. Araya Stephen O. Amoo Christian P. du Plooy Phatu W. Mashela |
| author_facet | Motiki M. Mofokeng Gerhard Prinsloo Hintsa T. Araya Stephen O. Amoo Christian P. du Plooy Phatu W. Mashela |
| author_sort | Motiki M. Mofokeng |
| collection | DOAJ |
| description | Soaking <i>Hypoxis hemerocallidea</i> corms in distilled water improved the propagation and development of cormlets, suggesting the potential leaching-out of inhibitory chemical compounds. To investigate the presence of inhibitory compounds, nuclear magnetic resonance (NMR) spectral data of the leachate from dormant <i>H. hemerocallidea</i> corms were obtained using a 600 MHz <sup>1</sup>H-NMR spectrometer. The <sup>1</sup>H-NMR analysis led to the identification of choline, succinate, propylene glycol, and lactose, as inhibitory compounds. These four chemical compounds are part of the “Natural Deep Eutectic Solvents” (NADES) that protect plant cells during stress periods, each of which has the potential to inhibit bud growth and development. These compounds are supposedly leached out of the corms during the first rain under natural conditions, possibly accompanied by changes in the ratios of dormancy-breaking phytohormones and inhibitory compounds, to release bud dormancy. The identified chemical compounds heralded a novel frontier in the vegetative propagation of <i>H. hemerocallidea</i> as a medicinal plant, and for its enhanced sustainable uses. |
| first_indexed | 2024-03-09T22:47:58Z |
| format | Article |
| id | doaj.art-faf63eaaff674e44b98685c0918b0da6 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T22:47:58Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-faf63eaaff674e44b98685c0918b0da62023-11-23T18:27:03ZengMDPI AGPlants2223-77472022-09-011118238710.3390/plants11182387NADES Compounds Identified in <i>Hypoxis hemerocallidea</i> Corms during DormancyMotiki M. Mofokeng0Gerhard Prinsloo1Hintsa T. Araya2Stephen O. Amoo3Christian P. du Plooy4Phatu W. Mashela5Agricultural Research Council—Vegetable, Industrial and Medicinal Plants (ARC-VIMP), Private Bag X293, Pretoria 0001, South AfricaDepartment of Agriculture and Animal Health, University of South Africa, Private Bag X6, Johannesburg 1710, South AfricaAgricultural Research Council—Vegetable, Industrial and Medicinal Plants (ARC-VIMP), Private Bag X293, Pretoria 0001, South AfricaAgricultural Research Council—Vegetable, Industrial and Medicinal Plants (ARC-VIMP), Private Bag X293, Pretoria 0001, South AfricaAgricultural Research Council—Vegetable, Industrial and Medicinal Plants (ARC-VIMP), Private Bag X293, Pretoria 0001, South AfricaGreen Technologies Research Centre, University of Limpopo, Private Bag X1106, Sovenga 0727, South AfricaSoaking <i>Hypoxis hemerocallidea</i> corms in distilled water improved the propagation and development of cormlets, suggesting the potential leaching-out of inhibitory chemical compounds. To investigate the presence of inhibitory compounds, nuclear magnetic resonance (NMR) spectral data of the leachate from dormant <i>H. hemerocallidea</i> corms were obtained using a 600 MHz <sup>1</sup>H-NMR spectrometer. The <sup>1</sup>H-NMR analysis led to the identification of choline, succinate, propylene glycol, and lactose, as inhibitory compounds. These four chemical compounds are part of the “Natural Deep Eutectic Solvents” (NADES) that protect plant cells during stress periods, each of which has the potential to inhibit bud growth and development. These compounds are supposedly leached out of the corms during the first rain under natural conditions, possibly accompanied by changes in the ratios of dormancy-breaking phytohormones and inhibitory compounds, to release bud dormancy. The identified chemical compounds heralded a novel frontier in the vegetative propagation of <i>H. hemerocallidea</i> as a medicinal plant, and for its enhanced sustainable uses.https://www.mdpi.com/2223-7747/11/18/2387propagationcholinelactoseorganic acidssuccinatepropylene glycol |
| spellingShingle | Motiki M. Mofokeng Gerhard Prinsloo Hintsa T. Araya Stephen O. Amoo Christian P. du Plooy Phatu W. Mashela NADES Compounds Identified in <i>Hypoxis hemerocallidea</i> Corms during Dormancy Plants propagation choline lactose organic acids succinate propylene glycol |
| title | NADES Compounds Identified in <i>Hypoxis hemerocallidea</i> Corms during Dormancy |
| title_full | NADES Compounds Identified in <i>Hypoxis hemerocallidea</i> Corms during Dormancy |
| title_fullStr | NADES Compounds Identified in <i>Hypoxis hemerocallidea</i> Corms during Dormancy |
| title_full_unstemmed | NADES Compounds Identified in <i>Hypoxis hemerocallidea</i> Corms during Dormancy |
| title_short | NADES Compounds Identified in <i>Hypoxis hemerocallidea</i> Corms during Dormancy |
| title_sort | nades compounds identified in i hypoxis hemerocallidea i corms during dormancy |
| topic | propagation choline lactose organic acids succinate propylene glycol |
| url | https://www.mdpi.com/2223-7747/11/18/2387 |
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