Engineering microdent structures of bone implant surfaces to enhance osteogenic activity in MSCs
Problems persist with the integration of hip and dental implants with host bone tissues, which may result in long-term implant failure. Previous studies have found that implants bearing irregular surfaces can facilitate osseointegration. An improvement to this approach would use implant surfaces har...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-03-01
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| Series: | Biochemistry and Biophysics Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580816303351 |
| _version_ | 1819143928386945024 |
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| author | Sophia Li Thomas Chow Julia Chu |
| author_facet | Sophia Li Thomas Chow Julia Chu |
| author_sort | Sophia Li |
| collection | DOAJ |
| description | Problems persist with the integration of hip and dental implants with host bone tissues, which may result in long-term implant failure. Previous studies have found that implants bearing irregular surfaces can facilitate osseointegration. An improvement to this approach would use implant surfaces harboring a well-defined surface microstructure to decrease variability in implant surfaces. In this study, we tested whether well-defined surfaces with arrays of microdents (each with depth approximately 3 µm) significantly affected the morphology, proliferation, and osteogenic activity of mesenchymal stem cells (MSCs). Arrays of microdents tested had diameters of 9 µm, 12 µm, and 18 µm, while spacing between arrays ranged from 8 µm to 34 µm. Effects on MSC morphology (cell spreading area) and proliferation were also quantified, with both significantly decreasing on micropatterned surfaces (p<0.05) on smaller and denser microdents. In contrast, MSCs were found to deposit more calcified matrix on smaller and denser arrays of microdents. MSCs on a pattern with arrays of microdents with a diameter of 9 µm and a spacing 8 µm deposited 3–4 times more calcified matrix than on a smooth surface (p<0.05). These findings show that well-defined surface microtopographies promote osteogenic activity, which can be used on implant surfaces to improve integration with the host bone tissue. |
| first_indexed | 2024-12-22T12:34:02Z |
| format | Article |
| id | doaj.art-fafbb96b491f451984ecb4d9aa8a45c0 |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-12-22T12:34:02Z |
| publishDate | 2017-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-fafbb96b491f451984ecb4d9aa8a45c02022-12-21T18:25:37ZengElsevierBiochemistry and Biophysics Reports2405-58082017-03-019C10010510.1016/j.bbrep.2016.11.016Engineering microdent structures of bone implant surfaces to enhance osteogenic activity in MSCsSophia LiThomas ChowJulia ChuProblems persist with the integration of hip and dental implants with host bone tissues, which may result in long-term implant failure. Previous studies have found that implants bearing irregular surfaces can facilitate osseointegration. An improvement to this approach would use implant surfaces harboring a well-defined surface microstructure to decrease variability in implant surfaces. In this study, we tested whether well-defined surfaces with arrays of microdents (each with depth approximately 3 µm) significantly affected the morphology, proliferation, and osteogenic activity of mesenchymal stem cells (MSCs). Arrays of microdents tested had diameters of 9 µm, 12 µm, and 18 µm, while spacing between arrays ranged from 8 µm to 34 µm. Effects on MSC morphology (cell spreading area) and proliferation were also quantified, with both significantly decreasing on micropatterned surfaces (p<0.05) on smaller and denser microdents. In contrast, MSCs were found to deposit more calcified matrix on smaller and denser arrays of microdents. MSCs on a pattern with arrays of microdents with a diameter of 9 µm and a spacing 8 µm deposited 3–4 times more calcified matrix than on a smooth surface (p<0.05). These findings show that well-defined surface microtopographies promote osteogenic activity, which can be used on implant surfaces to improve integration with the host bone tissue.http://www.sciencedirect.com/science/article/pii/S2405580816303351MicropatternsBone implantsMicrodentsMesenchymal stem cellsOsteogenic differentiation |
| spellingShingle | Sophia Li Thomas Chow Julia Chu Engineering microdent structures of bone implant surfaces to enhance osteogenic activity in MSCs Biochemistry and Biophysics Reports Micropatterns Bone implants Microdents Mesenchymal stem cells Osteogenic differentiation |
| title | Engineering microdent structures of bone implant surfaces to enhance osteogenic activity in MSCs |
| title_full | Engineering microdent structures of bone implant surfaces to enhance osteogenic activity in MSCs |
| title_fullStr | Engineering microdent structures of bone implant surfaces to enhance osteogenic activity in MSCs |
| title_full_unstemmed | Engineering microdent structures of bone implant surfaces to enhance osteogenic activity in MSCs |
| title_short | Engineering microdent structures of bone implant surfaces to enhance osteogenic activity in MSCs |
| title_sort | engineering microdent structures of bone implant surfaces to enhance osteogenic activity in mscs |
| topic | Micropatterns Bone implants Microdents Mesenchymal stem cells Osteogenic differentiation |
| url | http://www.sciencedirect.com/science/article/pii/S2405580816303351 |
| work_keys_str_mv | AT sophiali engineeringmicrodentstructuresofboneimplantsurfacestoenhanceosteogenicactivityinmscs AT thomaschow engineeringmicrodentstructuresofboneimplantsurfacestoenhanceosteogenicactivityinmscs AT juliachu engineeringmicrodentstructuresofboneimplantsurfacestoenhanceosteogenicactivityinmscs |