Analysis of Compositional Bias in a Commercial Phage Display Peptide Library by Next-Generation Sequencing

Analysis of Compositional Bias in a Commercial Phage Display Peptide Library by Next-Generation Sequencing

The principal presumption of phage display biopanning is that the naïve library contains an unbiased repertoire of peptides, and thus, the enriched variants derive from the affinity selection of an entirely random peptide pool. In the current study, we utilized deep sequencing to characterize the wi...

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Bibliographic Details
Main Authors: Ane Beth Sloth, Babak Bakhshinejad, Malte Jensen, Camilla Stavnsbjerg, Mikkel Baldtzer Liisberg, Maria Rossing, Andreas Kjaer
Format: Article
Language:English
Published: MDPI AG 2022-10-01
Series:Viruses
Subjects:
biopanning
compositional bias
deep sequencing
departure from randomness
M13 phage
next-generation sequencing
Online Access:https://www.mdpi.com/1999-4915/14/11/2402

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