Effects of mRNA amplification on gene expression ratios in cDNA experiments estimated by analysis of variance

Effects of mRNA amplification on gene expression ratios in cDNA experiments estimated by analysis of variance

<p>Abstract</p> <p>Background</p> <p>A limiting factor of cDNA microarray technology is the need for a substantial amount of RNA per labeling reaction. Thus, 20–200 micro-grams total RNA or 0.5–2 micro-grams poly (A) RNA is typically required for monitoring gene express...

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Bibliographic Details
Main Authors: Myklebost Ola, Liu Fang, Rue Håvard, Langaas Mette, Holden Marit, Løland Anders, Nygaard Vigdis, Fodstad Øystein, Hovig Eivind, Smith-Sørensen Birgitte
Format: Article
Language:English
Published: BMC 2003-03-01
Series:BMC Genomics
Subjects:
mRNA amplification
microarray
gene expression
multiple hypothesis testing
linear mixed effects model
Online Access:http://www.biomedcentral.com/1471-2164/4/11

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