The Potential Antipyretic Mechanism of Ellagic Acid with Brain Metabolomics Using Rats with Yeast-Induced Fever
Fever is caused by an increase in the heat production process when the body is under the action of a heat source or the dysfunction of the temperature center. Ellagic acid (EA) is a polyphenol dilactone that has anti-inflammatory, anti-tumor, and antioxidant activities. Male Sprague-Dawley rats were...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/27/8/2465 |
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| author | Fengfeng Xie Liba Xu Hua Zhu Yaling Chen Yinlan Li Lizhen Nong Yanfang Zeng Sijie Cen |
| author_facet | Fengfeng Xie Liba Xu Hua Zhu Yaling Chen Yinlan Li Lizhen Nong Yanfang Zeng Sijie Cen |
| author_sort | Fengfeng Xie |
| collection | DOAJ |
| description | Fever is caused by an increase in the heat production process when the body is under the action of a heat source or the dysfunction of the temperature center. Ellagic acid (EA) is a polyphenol dilactone that has anti-inflammatory, anti-tumor, and antioxidant activities. Male Sprague-Dawley rats were injected yeast to reproduce an experimental fever model (150 ± 20 g), and the rectal temperature and its change values were subsequently taken 19 h later; the excessive production of interleukin-1β (IL-1β) and prostaglandin2 (PGE2) induced by yeast was regulated to normal by EA administration. Rat brain metabolomics investigation of pyrexia and the antipyretic anti-inflammatory effect of EA was performed using Ultra-High-Performance Liquid Chromatography–Mass spectrometry (UPLC-MS). Twenty-six metabolites, as potential biomarkers, significantly altered metabolites that were found in pyretic rats, and eleven metabolites, as biomarkers of the antipyretic mechanism of EA, were significantly adjusted by EA to help relieve pyrexia, which was involved in glycerophospholipid metabolism and sphingolipid metabolism, etc. In conclusion, potential metabolic biomarkers in the brain shed light on the mechanism of EA’s antipyretic effects, mainly involving metabolic pathways, which may contribute to a further understanding of the therapeutic mechanisms of fever and therapeutic mechanism of EA. |
| first_indexed | 2024-03-09T04:22:29Z |
| format | Article |
| id | doaj.art-faff737ac7b74b2cb6ebc74b4b4f3488 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T04:22:29Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-faff737ac7b74b2cb6ebc74b4b4f34882023-12-03T13:46:19ZengMDPI AGMolecules1420-30492022-04-01278246510.3390/molecules27082465The Potential Antipyretic Mechanism of Ellagic Acid with Brain Metabolomics Using Rats with Yeast-Induced FeverFengfeng Xie0Liba Xu1Hua Zhu2Yaling Chen3Yinlan Li4Lizhen Nong5Yanfang Zeng6Sijie Cen7School of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530006, ChinaCollaborative Innovation Center of Zhuang and Yao Ethnic Medicine, Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine, Guangxi University of Chinese Medicine, Nanning 530200, ChinaSchool of Chemistry and Chemical Engineering, Guangxi University for Nationalities, Nanning 530006, ChinaCollaborative Innovation Center of Zhuang and Yao Ethnic Medicine, Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine, Guangxi University of Chinese Medicine, Nanning 530200, ChinaCollaborative Innovation Center of Zhuang and Yao Ethnic Medicine, Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine, Guangxi University of Chinese Medicine, Nanning 530200, ChinaCollaborative Innovation Center of Zhuang and Yao Ethnic Medicine, Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine, Guangxi University of Chinese Medicine, Nanning 530200, ChinaCollaborative Innovation Center of Zhuang and Yao Ethnic Medicine, Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine, Guangxi University of Chinese Medicine, Nanning 530200, ChinaCollaborative Innovation Center of Zhuang and Yao Ethnic Medicine, Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine, Guangxi University of Chinese Medicine, Nanning 530200, ChinaFever is caused by an increase in the heat production process when the body is under the action of a heat source or the dysfunction of the temperature center. Ellagic acid (EA) is a polyphenol dilactone that has anti-inflammatory, anti-tumor, and antioxidant activities. Male Sprague-Dawley rats were injected yeast to reproduce an experimental fever model (150 ± 20 g), and the rectal temperature and its change values were subsequently taken 19 h later; the excessive production of interleukin-1β (IL-1β) and prostaglandin2 (PGE2) induced by yeast was regulated to normal by EA administration. Rat brain metabolomics investigation of pyrexia and the antipyretic anti-inflammatory effect of EA was performed using Ultra-High-Performance Liquid Chromatography–Mass spectrometry (UPLC-MS). Twenty-six metabolites, as potential biomarkers, significantly altered metabolites that were found in pyretic rats, and eleven metabolites, as biomarkers of the antipyretic mechanism of EA, were significantly adjusted by EA to help relieve pyrexia, which was involved in glycerophospholipid metabolism and sphingolipid metabolism, etc. In conclusion, potential metabolic biomarkers in the brain shed light on the mechanism of EA’s antipyretic effects, mainly involving metabolic pathways, which may contribute to a further understanding of the therapeutic mechanisms of fever and therapeutic mechanism of EA.https://www.mdpi.com/1420-3049/27/8/2465fevermetabolomicsUPLC-MSellagic acidbiomarkers |
| spellingShingle | Fengfeng Xie Liba Xu Hua Zhu Yaling Chen Yinlan Li Lizhen Nong Yanfang Zeng Sijie Cen The Potential Antipyretic Mechanism of Ellagic Acid with Brain Metabolomics Using Rats with Yeast-Induced Fever Molecules fever metabolomics UPLC-MS ellagic acid biomarkers |
| title | The Potential Antipyretic Mechanism of Ellagic Acid with Brain Metabolomics Using Rats with Yeast-Induced Fever |
| title_full | The Potential Antipyretic Mechanism of Ellagic Acid with Brain Metabolomics Using Rats with Yeast-Induced Fever |
| title_fullStr | The Potential Antipyretic Mechanism of Ellagic Acid with Brain Metabolomics Using Rats with Yeast-Induced Fever |
| title_full_unstemmed | The Potential Antipyretic Mechanism of Ellagic Acid with Brain Metabolomics Using Rats with Yeast-Induced Fever |
| title_short | The Potential Antipyretic Mechanism of Ellagic Acid with Brain Metabolomics Using Rats with Yeast-Induced Fever |
| title_sort | potential antipyretic mechanism of ellagic acid with brain metabolomics using rats with yeast induced fever |
| topic | fever metabolomics UPLC-MS ellagic acid biomarkers |
| url | https://www.mdpi.com/1420-3049/27/8/2465 |
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