Development of an anti-oxidative intraocular irrigating solution based on reactive persulfides
Abstract Anti-oxidative intraocular irrigating solutions (IISs) based on reactive persulfides, such as oxidized glutathione disulfide (GSSG), are commonly used worldwide. However, even with GSSG-based IISs, it has been shown that oxidative stress can occur during surgery, posing a risk to intraocula...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-21677-4 |
| _version_ | 1828129556729430016 |
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| author | Hiroshi Kunikata Hiroshi Tawarayama Satoru Tsuda Takaaki Akaike Toru Nakazawa |
| author_facet | Hiroshi Kunikata Hiroshi Tawarayama Satoru Tsuda Takaaki Akaike Toru Nakazawa |
| author_sort | Hiroshi Kunikata |
| collection | DOAJ |
| description | Abstract Anti-oxidative intraocular irrigating solutions (IISs) based on reactive persulfides, such as oxidized glutathione disulfide (GSSG), are commonly used worldwide. However, even with GSSG-based IISs, it has been shown that oxidative stress can occur during surgery, posing a risk to intraocular tissues. This study compared two IISs: one containing GSSG and one containing an oxidized glutathione trisulfide (GSSSG). Experimental in vivo irrigation with the IISs in rabbits showed that there was less leakage into the anterior chamber of rabbit serum albumin during perfusion with a 300-μM GSSSG IIS than with a 300-μM GSSG IIS. Experimental in vivo cataract surgery in rabbits showed that aqueous flare was suppressed 3 days after surgery with a 600-μM GSSSG IIS, but not with a 300-μM GSSSG or 300-μM GSSG IIS. Furthermore, an in vitro experiment, without any live tissue, showed that reactive oxygen species were suppressed more strongly with a 600-μM GSSSG IIS than with a 300-μM GSSG IIS. Thus, this study found that novel IISs based on GSSSG had anti-inflammatory and anti-oxidative effects during and after intraocular surgery and may decrease the rate of complications after surgery. |
| first_indexed | 2024-04-11T16:20:27Z |
| format | Article |
| id | doaj.art-faff876ecb3c449ead196181b3681c44 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T16:20:27Z |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-faff876ecb3c449ead196181b3681c442022-12-22T04:14:24ZengNature PortfolioScientific Reports2045-23222022-11-011211710.1038/s41598-022-21677-4Development of an anti-oxidative intraocular irrigating solution based on reactive persulfidesHiroshi Kunikata0Hiroshi Tawarayama1Satoru Tsuda2Takaaki Akaike3Toru Nakazawa4Department of Ophthalmology, Tohoku University Graduate School of MedicineDepartment of Retinal Disease Control, Tohoku University Graduate School of MedicineDepartment of Ophthalmology, Tohoku University Graduate School of MedicineDepartment of Environmental Health Sciences and Molecular Toxicology, Tohoku University Graduate School of MedicineDepartment of Ophthalmology, Tohoku University Graduate School of MedicineAbstract Anti-oxidative intraocular irrigating solutions (IISs) based on reactive persulfides, such as oxidized glutathione disulfide (GSSG), are commonly used worldwide. However, even with GSSG-based IISs, it has been shown that oxidative stress can occur during surgery, posing a risk to intraocular tissues. This study compared two IISs: one containing GSSG and one containing an oxidized glutathione trisulfide (GSSSG). Experimental in vivo irrigation with the IISs in rabbits showed that there was less leakage into the anterior chamber of rabbit serum albumin during perfusion with a 300-μM GSSSG IIS than with a 300-μM GSSG IIS. Experimental in vivo cataract surgery in rabbits showed that aqueous flare was suppressed 3 days after surgery with a 600-μM GSSSG IIS, but not with a 300-μM GSSSG or 300-μM GSSG IIS. Furthermore, an in vitro experiment, without any live tissue, showed that reactive oxygen species were suppressed more strongly with a 600-μM GSSSG IIS than with a 300-μM GSSG IIS. Thus, this study found that novel IISs based on GSSSG had anti-inflammatory and anti-oxidative effects during and after intraocular surgery and may decrease the rate of complications after surgery.https://doi.org/10.1038/s41598-022-21677-4 |
| spellingShingle | Hiroshi Kunikata Hiroshi Tawarayama Satoru Tsuda Takaaki Akaike Toru Nakazawa Development of an anti-oxidative intraocular irrigating solution based on reactive persulfides Scientific Reports |
| title | Development of an anti-oxidative intraocular irrigating solution based on reactive persulfides |
| title_full | Development of an anti-oxidative intraocular irrigating solution based on reactive persulfides |
| title_fullStr | Development of an anti-oxidative intraocular irrigating solution based on reactive persulfides |
| title_full_unstemmed | Development of an anti-oxidative intraocular irrigating solution based on reactive persulfides |
| title_short | Development of an anti-oxidative intraocular irrigating solution based on reactive persulfides |
| title_sort | development of an anti oxidative intraocular irrigating solution based on reactive persulfides |
| url | https://doi.org/10.1038/s41598-022-21677-4 |
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