Genetic diversity in different populations of citrus leafminer, Phyllocnistis citrella Stainton (Lepidoptera: Gracillariidae) in Tunisia, assessed by RAPD-PCR
The citrus leafminer, Phyllocnistis citrella Stainton (Lepidoptera: Gracillariidae) is a major invasive pest of citrus in Tunisia. In order to help the implementation of an efficient integrated management strategy, it was essential to assess the genetic diversity and population structure of the pest...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
University of Tarbiat Modares
2012-09-01
|
| Series: | Journal of Crop Protection |
| Subjects: | |
| Online Access: | http://jcp.modares.ac.ir/article-3-12180-en.html |
| _version_ | 1818474613522300928 |
|---|---|
| author | Dhia Bouktila Saïda Kharrat Maha Mezghani-Khemakhem Abderrahmane Jerraya Mohamed Makni |
| author_facet | Dhia Bouktila Saïda Kharrat Maha Mezghani-Khemakhem Abderrahmane Jerraya Mohamed Makni |
| author_sort | Dhia Bouktila |
| collection | DOAJ |
| description | The citrus leafminer, Phyllocnistis citrella Stainton (Lepidoptera: Gracillariidae) is a major invasive pest of citrus in Tunisia. In order to help the implementation of an efficient integrated management strategy, it was essential to assess the genetic diversity and population structure of the pest. For this purpose, random-amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was applied, using eight oligo-nucleotide primers, to reveal genetic variability among eight populations of P. citrella, originating from the north, center and south of Tunisia. A total of 66 RAPD markers and 33 phenotypes were generated. Inter-population polymorphism was revealed, using the percentage of polymorphic markers (62.12 %), mean number of phenotypes generated per primer (4.125) and mean genetic distance (0.199). Hierarchical analysis, using the UPGMA method, indicated that the genetic variability was influenced by the regional distribution. This pattern of population clustering was supported by Principal Coordinate Analysis (PCO). Yet, a weak correlation (0.69) was revealed between genetic and geographic distances, suggesting that climatic contrariety between the north and south of Tunisia plays a major role in the differentiation of P. citrella, leading to a restriction of gene flow between populations. Results obtained in this work show clear genetic differences, which should be considered in the development of control strategies. |
| first_indexed | 2024-04-14T04:39:33Z |
| format | Article |
| id | doaj.art-fb011347789a429fa0a3ff7bf00153a7 |
| institution | Directory Open Access Journal |
| issn | 2251-9041 2251-905X |
| language | English |
| last_indexed | 2024-04-14T04:39:33Z |
| publishDate | 2012-09-01 |
| publisher | University of Tarbiat Modares |
| record_format | Article |
| series | Journal of Crop Protection |
| spelling | doaj.art-fb011347789a429fa0a3ff7bf00153a72022-12-22T02:11:45ZengUniversity of Tarbiat ModaresJournal of Crop Protection2251-90412251-905X2012-09-0113189199Genetic diversity in different populations of citrus leafminer, Phyllocnistis citrella Stainton (Lepidoptera: Gracillariidae) in Tunisia, assessed by RAPD-PCRDhia Bouktila0Saïda Kharrat1Maha Mezghani-Khemakhem2Abderrahmane Jerraya3Mohamed Makni4 Higher Institute of Biotechnology of Béja. University of Jendouba, Tunisia Faculty of Sciences of Bizerte. University of Cartago. Tunisia Research Unit on Genomics of Crop Insect Pests (GIRC), Faculty of Sciences of Tunis, University of Tunis-El-Manar, Tunisia Department of Entomology, National Institute of Agronomy, Tunis, Tunisia Research Unit on Genomics of Crop Insect Pests (GIRC), Faculty of Sciences of Tunis, University of Tunis-El-Manar, Tunisia The citrus leafminer, Phyllocnistis citrella Stainton (Lepidoptera: Gracillariidae) is a major invasive pest of citrus in Tunisia. In order to help the implementation of an efficient integrated management strategy, it was essential to assess the genetic diversity and population structure of the pest. For this purpose, random-amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) was applied, using eight oligo-nucleotide primers, to reveal genetic variability among eight populations of P. citrella, originating from the north, center and south of Tunisia. A total of 66 RAPD markers and 33 phenotypes were generated. Inter-population polymorphism was revealed, using the percentage of polymorphic markers (62.12 %), mean number of phenotypes generated per primer (4.125) and mean genetic distance (0.199). Hierarchical analysis, using the UPGMA method, indicated that the genetic variability was influenced by the regional distribution. This pattern of population clustering was supported by Principal Coordinate Analysis (PCO). Yet, a weak correlation (0.69) was revealed between genetic and geographic distances, suggesting that climatic contrariety between the north and south of Tunisia plays a major role in the differentiation of P. citrella, leading to a restriction of gene flow between populations. Results obtained in this work show clear genetic differences, which should be considered in the development of control strategies.http://jcp.modares.ac.ir/article-3-12180-en.htmlcitrusgenetic diversitypest managementphyllocnistis citrellarapd-pcr |
| spellingShingle | Dhia Bouktila Saïda Kharrat Maha Mezghani-Khemakhem Abderrahmane Jerraya Mohamed Makni Genetic diversity in different populations of citrus leafminer, Phyllocnistis citrella Stainton (Lepidoptera: Gracillariidae) in Tunisia, assessed by RAPD-PCR Journal of Crop Protection citrus genetic diversity pest management phyllocnistis citrella rapd-pcr |
| title | Genetic diversity in different populations of citrus leafminer, Phyllocnistis citrella Stainton (Lepidoptera: Gracillariidae) in Tunisia, assessed by RAPD-PCR |
| title_full | Genetic diversity in different populations of citrus leafminer, Phyllocnistis citrella Stainton (Lepidoptera: Gracillariidae) in Tunisia, assessed by RAPD-PCR |
| title_fullStr | Genetic diversity in different populations of citrus leafminer, Phyllocnistis citrella Stainton (Lepidoptera: Gracillariidae) in Tunisia, assessed by RAPD-PCR |
| title_full_unstemmed | Genetic diversity in different populations of citrus leafminer, Phyllocnistis citrella Stainton (Lepidoptera: Gracillariidae) in Tunisia, assessed by RAPD-PCR |
| title_short | Genetic diversity in different populations of citrus leafminer, Phyllocnistis citrella Stainton (Lepidoptera: Gracillariidae) in Tunisia, assessed by RAPD-PCR |
| title_sort | genetic diversity in different populations of citrus leafminer phyllocnistis citrella stainton lepidoptera gracillariidae in tunisia assessed by rapd pcr |
| topic | citrus genetic diversity pest management phyllocnistis citrella rapd-pcr |
| url | http://jcp.modares.ac.ir/article-3-12180-en.html |
| work_keys_str_mv | AT dhiabouktila geneticdiversityindifferentpopulationsofcitrusleafminerphyllocnistiscitrellastaintonlepidopteragracillariidaeintunisiaassessedbyrapdpcr AT saidakharrat geneticdiversityindifferentpopulationsofcitrusleafminerphyllocnistiscitrellastaintonlepidopteragracillariidaeintunisiaassessedbyrapdpcr AT mahamezghanikhemakhem geneticdiversityindifferentpopulationsofcitrusleafminerphyllocnistiscitrellastaintonlepidopteragracillariidaeintunisiaassessedbyrapdpcr AT abderrahmanejerraya geneticdiversityindifferentpopulationsofcitrusleafminerphyllocnistiscitrellastaintonlepidopteragracillariidaeintunisiaassessedbyrapdpcr AT mohamedmakni geneticdiversityindifferentpopulationsofcitrusleafminerphyllocnistiscitrellastaintonlepidopteragracillariidaeintunisiaassessedbyrapdpcr |