Model structure of human APOBEC3G.
APOBEC3G (apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G) has antiretroviral activity associated with the hypermutation of viral DNA through cytosine deamination. APOBEC3G has two cytosine deaminase (CDA) domains; the catalytically inactive amino-terminal domain of APOBEC3G (N-C...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2007-04-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC1849894?pdf=render |
| _version_ | 1819091161599442944 |
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| author | Kun-Lin Zhang Bastien Mangeat Millan Ortiz Vincent Zoete Didier Trono Amalio Telenti Olivier Michielin |
| author_facet | Kun-Lin Zhang Bastien Mangeat Millan Ortiz Vincent Zoete Didier Trono Amalio Telenti Olivier Michielin |
| author_sort | Kun-Lin Zhang |
| collection | DOAJ |
| description | APOBEC3G (apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G) has antiretroviral activity associated with the hypermutation of viral DNA through cytosine deamination. APOBEC3G has two cytosine deaminase (CDA) domains; the catalytically inactive amino-terminal domain of APOBEC3G (N-CDA) carries the Vif interaction domain. There is no 3-D structure of APOBEC3G solved by X-ray or nuclear magnetic resonance.We predicted the structure of human APOBEC3G based on the crystal structure of APOBEC2. To assess the model structure, we evaluated 48 mutants of APOBEC3G N-CDA that identify novel variants altering DeltaVif HIV-1 infectivity and packaging of APOBEC3G. Results indicated that the key residue D128 is exposed at the surface of the model, with a negative local electrostatic potential. Mutation D128K changes the sign of that local potential. In addition, two novel functionally relevant residues that result in defective APOBEC3G encapsidation, R122 and W127, cluster at the surface.The structure model identifies a cluster of residues important for packaging of APOBEC3G into virions, and may serve to guide functional analysis of APOBEC3G. |
| first_indexed | 2024-12-21T22:35:19Z |
| format | Article |
| id | doaj.art-fb0246ab8b2747609402aa97536c7f8f |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T22:35:19Z |
| publishDate | 2007-04-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-fb0246ab8b2747609402aa97536c7f8f2022-12-21T18:47:59ZengPublic Library of Science (PLoS)PLoS ONE1932-62032007-04-0124e37810.1371/journal.pone.0000378Model structure of human APOBEC3G.Kun-Lin ZhangBastien MangeatMillan OrtizVincent ZoeteDidier TronoAmalio TelentiOlivier MichielinAPOBEC3G (apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-like 3G) has antiretroviral activity associated with the hypermutation of viral DNA through cytosine deamination. APOBEC3G has two cytosine deaminase (CDA) domains; the catalytically inactive amino-terminal domain of APOBEC3G (N-CDA) carries the Vif interaction domain. There is no 3-D structure of APOBEC3G solved by X-ray or nuclear magnetic resonance.We predicted the structure of human APOBEC3G based on the crystal structure of APOBEC2. To assess the model structure, we evaluated 48 mutants of APOBEC3G N-CDA that identify novel variants altering DeltaVif HIV-1 infectivity and packaging of APOBEC3G. Results indicated that the key residue D128 is exposed at the surface of the model, with a negative local electrostatic potential. Mutation D128K changes the sign of that local potential. In addition, two novel functionally relevant residues that result in defective APOBEC3G encapsidation, R122 and W127, cluster at the surface.The structure model identifies a cluster of residues important for packaging of APOBEC3G into virions, and may serve to guide functional analysis of APOBEC3G.http://europepmc.org/articles/PMC1849894?pdf=render |
| spellingShingle | Kun-Lin Zhang Bastien Mangeat Millan Ortiz Vincent Zoete Didier Trono Amalio Telenti Olivier Michielin Model structure of human APOBEC3G. PLoS ONE |
| title | Model structure of human APOBEC3G. |
| title_full | Model structure of human APOBEC3G. |
| title_fullStr | Model structure of human APOBEC3G. |
| title_full_unstemmed | Model structure of human APOBEC3G. |
| title_short | Model structure of human APOBEC3G. |
| title_sort | model structure of human apobec3g |
| url | http://europepmc.org/articles/PMC1849894?pdf=render |
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