Pancreatic Islet Purification from Large Mammals and Humans Using a COBE 2991 Cell Processor versus Large Plastic Bottles
The islet purification step in clinical islet isolation is important for minimizing the risks associated with intraportal infusion. Continuous density gradient with a COBE 2991 cell processor is commonly used for clinical islet purification. However, the high shear force involved in the purification...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/10/1/10 |
| _version_ | 1797543812969529344 |
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| author | Hirofumi Noguchi |
| author_facet | Hirofumi Noguchi |
| author_sort | Hirofumi Noguchi |
| collection | DOAJ |
| description | The islet purification step in clinical islet isolation is important for minimizing the risks associated with intraportal infusion. Continuous density gradient with a COBE 2991 cell processor is commonly used for clinical islet purification. However, the high shear force involved in the purification method using the COBE 2991 cell processor causes mechanical damage to the islets. We and other groups have shown human/porcine islet purification using large cylindrical plastic bottles. Shear stress can be minimized or eliminated using large cylindrical plastic bottles because the bottles do not have a narrow segment and no centrifugation is required during tissue loading and the collection processes of islet purification. This review describes current advances in islet purification from large mammals and humans using a COBE 2991 cell processor versus large cylindrical plastic bottles. |
| first_indexed | 2024-03-10T13:51:16Z |
| format | Article |
| id | doaj.art-fb039cd130e84ccc8431c0aaeb361229 |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T13:51:16Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-fb039cd130e84ccc8431c0aaeb3612292023-11-21T02:08:36ZengMDPI AGJournal of Clinical Medicine2077-03832020-12-011011010.3390/jcm10010010Pancreatic Islet Purification from Large Mammals and Humans Using a COBE 2991 Cell Processor versus Large Plastic BottlesHirofumi Noguchi0Department of Regenerative Medicine, Graduate School of Medicine, University of the Ryukyus, Okinawa 903-0215, JapanThe islet purification step in clinical islet isolation is important for minimizing the risks associated with intraportal infusion. Continuous density gradient with a COBE 2991 cell processor is commonly used for clinical islet purification. However, the high shear force involved in the purification method using the COBE 2991 cell processor causes mechanical damage to the islets. We and other groups have shown human/porcine islet purification using large cylindrical plastic bottles. Shear stress can be minimized or eliminated using large cylindrical plastic bottles because the bottles do not have a narrow segment and no centrifugation is required during tissue loading and the collection processes of islet purification. This review describes current advances in islet purification from large mammals and humans using a COBE 2991 cell processor versus large cylindrical plastic bottles.https://www.mdpi.com/2077-0383/10/1/10islet transplantationislet purificationCOBE 2991 cell processorlarge cylindrical plastic bottlescontinuous density gradient |
| spellingShingle | Hirofumi Noguchi Pancreatic Islet Purification from Large Mammals and Humans Using a COBE 2991 Cell Processor versus Large Plastic Bottles Journal of Clinical Medicine islet transplantation islet purification COBE 2991 cell processor large cylindrical plastic bottles continuous density gradient |
| title | Pancreatic Islet Purification from Large Mammals and Humans Using a COBE 2991 Cell Processor versus Large Plastic Bottles |
| title_full | Pancreatic Islet Purification from Large Mammals and Humans Using a COBE 2991 Cell Processor versus Large Plastic Bottles |
| title_fullStr | Pancreatic Islet Purification from Large Mammals and Humans Using a COBE 2991 Cell Processor versus Large Plastic Bottles |
| title_full_unstemmed | Pancreatic Islet Purification from Large Mammals and Humans Using a COBE 2991 Cell Processor versus Large Plastic Bottles |
| title_short | Pancreatic Islet Purification from Large Mammals and Humans Using a COBE 2991 Cell Processor versus Large Plastic Bottles |
| title_sort | pancreatic islet purification from large mammals and humans using a cobe 2991 cell processor versus large plastic bottles |
| topic | islet transplantation islet purification COBE 2991 cell processor large cylindrical plastic bottles continuous density gradient |
| url | https://www.mdpi.com/2077-0383/10/1/10 |
| work_keys_str_mv | AT hirofuminoguchi pancreaticisletpurificationfromlargemammalsandhumansusingacobe2991cellprocessorversuslargeplasticbottles |