Synthesis and Antibacterial Activity of Manganese-Ferrite/Silver Nanocomposite Combined with Two Essential Oils
The antimicrobial activity of metal nanoparticles obtained by biogenic routes has been extensively reported. However, their combined use with other antimicrobial formulations, such as essential oils, remains scarcely explored. In this work, a manganese-ferrite/silver nanocomposite (MnFe<sub>2&...
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MDPI AG
2022-06-01
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| Series: | Nanomaterials |
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| Online Access: | https://www.mdpi.com/2079-4991/12/13/2137 |
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| author | Javiera Parada Marcela Díaz Edward Hermosilla Joelis Vera Gonzalo Tortella Amedea B. Seabra Andrés Quiroz Emilio Hormazábal Olga Rubilar |
| author_facet | Javiera Parada Marcela Díaz Edward Hermosilla Joelis Vera Gonzalo Tortella Amedea B. Seabra Andrés Quiroz Emilio Hormazábal Olga Rubilar |
| author_sort | Javiera Parada |
| collection | DOAJ |
| description | The antimicrobial activity of metal nanoparticles obtained by biogenic routes has been extensively reported. However, their combined use with other antimicrobial formulations, such as essential oils, remains scarcely explored. In this work, a manganese-ferrite/silver nanocomposite (MnFe<sub>2</sub>O<sub>4</sub>/Ag-NC) was synthesized in a two-step procedure: first, MnFe<sub>2</sub>O<sub>4</sub> nanoparticles were produced by a coprecipitation method, followed by <i>in situ</i> biogenic reduction of silver ions using <i>Galega officinalis</i>. MnFe<sub>2</sub>O<sub>4</sub>/Ag-NC was characterized using transmission electron microscopy (TEM), scanning electron microscopy equipped with an energy dispersive X-ray analyzer (SEM-EDX), and a vibrating sample magnetometer (VSM-SQUID). The antibacterial activity if MnFe<sub>2</sub>O<sub>4</sub>/Ag-NC was evaluated against <i>Pseudomonas syringae</i> by determining its minimum inhibitory concentration (MIC) in the presence of two essential oils: eucalyptus oil (EO) and garlic oil (GO). The fractional inhibitory concentration (FIC) was also calculated to determine the interaction between MnFe<sub>2</sub>O<sub>4</sub>/Ag-NC and each oil. The MIC of MnFe<sub>2</sub>O<sub>4</sub>/Ag-NC was eightfold reduced with the two essential oils (from 20 to 2.5 µg mL<sup>−1</sup>). However, the interaction with EO was synergistic (FIC: 0.5), whereas the interaction with GO was additive (FIC: 0.75). Additionally, a time-kill curve analysis was performed, wherein the MIC of the combination of MnFe<sub>2</sub>O<sub>4</sub>/Ag-NC and EO provoked a rapid bactericidal effect, corroborating a strong synergism. These findings suggest that by combining MnFe<sub>2</sub>O<sub>4</sub>/Ag-NC with essential oils, the necessary ratio of the nanocomposite to control phytopathogens can be reduced, thus minimizing the environmental release of silver. |
| first_indexed | 2024-03-09T10:26:47Z |
| format | Article |
| id | doaj.art-fb0565b6471140b1bfaf99107f74d6b8 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T10:26:47Z |
| publishDate | 2022-06-01 |
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| series | Nanomaterials |
| spelling | doaj.art-fb0565b6471140b1bfaf99107f74d6b82023-12-01T21:37:13ZengMDPI AGNanomaterials2079-49912022-06-011213213710.3390/nano12132137Synthesis and Antibacterial Activity of Manganese-Ferrite/Silver Nanocomposite Combined with Two Essential OilsJaviera Parada0Marcela Díaz1Edward Hermosilla2Joelis Vera3Gonzalo Tortella4Amedea B. Seabra5Andrés Quiroz6Emilio Hormazábal7Olga Rubilar8Chemical Engineering Department, Universidad de La Frontera, Temuco P.O. Box 54-D, ChileBiotechnological Research Center Applied to the Environment (CIBAMA-BIOREN), Universidad de La Frontera, Temuco P.O. Box 54-D, ChileChemical Engineering Department, Universidad de La Frontera, Temuco P.O. Box 54-D, ChileBiotechnological Research Center Applied to the Environment (CIBAMA-BIOREN), Universidad de La Frontera, Temuco P.O. Box 54-D, ChileChemical Engineering Department, Universidad de La Frontera, Temuco P.O. Box 54-D, ChileCenter for Natural and Human Sciences, Federal University of ABC (UFABC), Santo André 09210-580, BrazilDepartamento de Ciencias Químicas y Recursos Naturales, Universidad de La Frontera, Temuco P.O. Box 54-D, ChileDepartamento de Ciencias Químicas y Recursos Naturales, Universidad de La Frontera, Temuco P.O. Box 54-D, ChileChemical Engineering Department, Universidad de La Frontera, Temuco P.O. Box 54-D, ChileThe antimicrobial activity of metal nanoparticles obtained by biogenic routes has been extensively reported. However, their combined use with other antimicrobial formulations, such as essential oils, remains scarcely explored. In this work, a manganese-ferrite/silver nanocomposite (MnFe<sub>2</sub>O<sub>4</sub>/Ag-NC) was synthesized in a two-step procedure: first, MnFe<sub>2</sub>O<sub>4</sub> nanoparticles were produced by a coprecipitation method, followed by <i>in situ</i> biogenic reduction of silver ions using <i>Galega officinalis</i>. MnFe<sub>2</sub>O<sub>4</sub>/Ag-NC was characterized using transmission electron microscopy (TEM), scanning electron microscopy equipped with an energy dispersive X-ray analyzer (SEM-EDX), and a vibrating sample magnetometer (VSM-SQUID). The antibacterial activity if MnFe<sub>2</sub>O<sub>4</sub>/Ag-NC was evaluated against <i>Pseudomonas syringae</i> by determining its minimum inhibitory concentration (MIC) in the presence of two essential oils: eucalyptus oil (EO) and garlic oil (GO). The fractional inhibitory concentration (FIC) was also calculated to determine the interaction between MnFe<sub>2</sub>O<sub>4</sub>/Ag-NC and each oil. The MIC of MnFe<sub>2</sub>O<sub>4</sub>/Ag-NC was eightfold reduced with the two essential oils (from 20 to 2.5 µg mL<sup>−1</sup>). However, the interaction with EO was synergistic (FIC: 0.5), whereas the interaction with GO was additive (FIC: 0.75). Additionally, a time-kill curve analysis was performed, wherein the MIC of the combination of MnFe<sub>2</sub>O<sub>4</sub>/Ag-NC and EO provoked a rapid bactericidal effect, corroborating a strong synergism. These findings suggest that by combining MnFe<sub>2</sub>O<sub>4</sub>/Ag-NC with essential oils, the necessary ratio of the nanocomposite to control phytopathogens can be reduced, thus minimizing the environmental release of silver.https://www.mdpi.com/2079-4991/12/13/2137manganese-ferrite nanoparticlessilver nanoparticlesnanocompositeantibacterialessential oils |
| spellingShingle | Javiera Parada Marcela Díaz Edward Hermosilla Joelis Vera Gonzalo Tortella Amedea B. Seabra Andrés Quiroz Emilio Hormazábal Olga Rubilar Synthesis and Antibacterial Activity of Manganese-Ferrite/Silver Nanocomposite Combined with Two Essential Oils Nanomaterials manganese-ferrite nanoparticles silver nanoparticles nanocomposite antibacterial essential oils |
| title | Synthesis and Antibacterial Activity of Manganese-Ferrite/Silver Nanocomposite Combined with Two Essential Oils |
| title_full | Synthesis and Antibacterial Activity of Manganese-Ferrite/Silver Nanocomposite Combined with Two Essential Oils |
| title_fullStr | Synthesis and Antibacterial Activity of Manganese-Ferrite/Silver Nanocomposite Combined with Two Essential Oils |
| title_full_unstemmed | Synthesis and Antibacterial Activity of Manganese-Ferrite/Silver Nanocomposite Combined with Two Essential Oils |
| title_short | Synthesis and Antibacterial Activity of Manganese-Ferrite/Silver Nanocomposite Combined with Two Essential Oils |
| title_sort | synthesis and antibacterial activity of manganese ferrite silver nanocomposite combined with two essential oils |
| topic | manganese-ferrite nanoparticles silver nanoparticles nanocomposite antibacterial essential oils |
| url | https://www.mdpi.com/2079-4991/12/13/2137 |
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