A Glycosyltransferase-Related Signature for Predicting Overall Survival in Head and Neck Squamous Cell Carcinoma
Background: Here, we establish a prognostic signature based on glycosyltransferase-related genes (GTRGs) for head and neck squamous cell carcinoma (HNSCC) patients.Methods: The prognostic signature of GTRGs was constructed via univariate and multivariate Cox analyses after obtaining the expression p...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-07-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2022.856671/full |
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| author | Huili Wu Huili Wu Huili Wu Huili Wu Xiao Zhao Xiao Zhao Xiao Zhao Xiao Zhao Tingting Zhu Tingting Zhu Tingting Zhu Tingting Zhu Di Rong Di Rong Di Rong Di Rong Ying Wang Ying Wang Ying Wang Ying Wang Diya Leng Diya Leng Diya Leng Diya Leng Daming Wu Daming Wu Daming Wu Daming Wu |
| author_facet | Huili Wu Huili Wu Huili Wu Huili Wu Xiao Zhao Xiao Zhao Xiao Zhao Xiao Zhao Tingting Zhu Tingting Zhu Tingting Zhu Tingting Zhu Di Rong Di Rong Di Rong Di Rong Ying Wang Ying Wang Ying Wang Ying Wang Diya Leng Diya Leng Diya Leng Diya Leng Daming Wu Daming Wu Daming Wu Daming Wu |
| author_sort | Huili Wu |
| collection | DOAJ |
| description | Background: Here, we establish a prognostic signature based on glycosyltransferase-related genes (GTRGs) for head and neck squamous cell carcinoma (HNSCC) patients.Methods: The prognostic signature of GTRGs was constructed via univariate and multivariate Cox analyses after obtaining the expression patterns of GTRGs from the TCGA. A nomogram based on the signature and clinical parameters was established to predict the survival of each HNSCC patient. Potential mechanisms were explored through gene set enrichment analysis (GSEA) and immune cell infiltration, immune checkpoints, immunotherapy, and tumor mutational burden (TMB) analyses. The expression differences and prognostic efficacy of the signature were verified through the gene expression omnibus (GEO) and several online databases.Results: The prognostic signature was constructed based on five glycosyltransferases (PYGL, ALG3, EXT2, FUT2, and KDELC1) and validated in the GSE65858 dataset. The pathways enriched in the high- and low-risk groups were significantly different. The high-risk group had higher tumor purity; lower infiltration of immune cells, such as CD8+ T cells and Tregs; higher cancer-associated fibroblast (CAF) infiltration; lower immune function; and lower checkpoint expression. The signature can also be applied to distinguish whether patients benefit from immunotherapy. In addition, the high-risk group had a higher TMB and more gene mutations, including those in TP53, CSMD1, CDKN2A, and MUC17.Conclusion: We propose a prognostic signature based on glycosyltransferases for HNSCC patients that may provide potential targets and biomarkers for the precise treatment of HNSCC. |
| first_indexed | 2024-04-13T13:56:05Z |
| format | Article |
| id | doaj.art-fb06aa6cf9c44de5a78ddca1a233abe7 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-04-13T13:56:05Z |
| publishDate | 2022-07-01 |
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| series | Frontiers in Genetics |
| spelling | doaj.art-fb06aa6cf9c44de5a78ddca1a233abe72022-12-22T02:44:12ZengFrontiers Media S.A.Frontiers in Genetics1664-80212022-07-011310.3389/fgene.2022.856671856671A Glycosyltransferase-Related Signature for Predicting Overall Survival in Head and Neck Squamous Cell CarcinomaHuili Wu0Huili Wu1Huili Wu2Huili Wu3Xiao Zhao4Xiao Zhao5Xiao Zhao6Xiao Zhao7Tingting Zhu8Tingting Zhu9Tingting Zhu10Tingting Zhu11Di Rong12Di Rong13Di Rong14Di Rong15Ying Wang16Ying Wang17Ying Wang18Ying Wang19Diya Leng20Diya Leng21Diya Leng22Diya Leng23Daming Wu24Daming Wu25Daming Wu26Daming Wu27Department of Endodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, ChinaDepartment of Oral and Maxillofacial Imaging, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, ChinaJiangsu Province Key Laboratory of Oral Diseases, Nanjing, ChinaJiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, ChinaDepartment of Endodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, ChinaDepartment of Oral and Maxillofacial Imaging, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, ChinaJiangsu Province Key Laboratory of Oral Diseases, Nanjing, ChinaJiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, ChinaDepartment of Endodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, ChinaDepartment of Oral and Maxillofacial Imaging, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, ChinaJiangsu Province Key Laboratory of Oral Diseases, Nanjing, ChinaJiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, ChinaDepartment of Endodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, ChinaDepartment of Oral and Maxillofacial Imaging, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, ChinaJiangsu Province Key Laboratory of Oral Diseases, Nanjing, ChinaJiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, ChinaDepartment of Endodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, ChinaDepartment of Oral and Maxillofacial Imaging, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, ChinaJiangsu Province Key Laboratory of Oral Diseases, Nanjing, ChinaJiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, ChinaDepartment of Endodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, ChinaDepartment of Oral and Maxillofacial Imaging, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, ChinaJiangsu Province Key Laboratory of Oral Diseases, Nanjing, ChinaJiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, ChinaDepartment of Endodontics, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, ChinaDepartment of Oral and Maxillofacial Imaging, The Affiliated Stomatological Hospital of Nanjing Medical University, Nanjing, ChinaJiangsu Province Key Laboratory of Oral Diseases, Nanjing, ChinaJiangsu Province Engineering Research Center of Stomatological Translational Medicine, Nanjing, ChinaBackground: Here, we establish a prognostic signature based on glycosyltransferase-related genes (GTRGs) for head and neck squamous cell carcinoma (HNSCC) patients.Methods: The prognostic signature of GTRGs was constructed via univariate and multivariate Cox analyses after obtaining the expression patterns of GTRGs from the TCGA. A nomogram based on the signature and clinical parameters was established to predict the survival of each HNSCC patient. Potential mechanisms were explored through gene set enrichment analysis (GSEA) and immune cell infiltration, immune checkpoints, immunotherapy, and tumor mutational burden (TMB) analyses. The expression differences and prognostic efficacy of the signature were verified through the gene expression omnibus (GEO) and several online databases.Results: The prognostic signature was constructed based on five glycosyltransferases (PYGL, ALG3, EXT2, FUT2, and KDELC1) and validated in the GSE65858 dataset. The pathways enriched in the high- and low-risk groups were significantly different. The high-risk group had higher tumor purity; lower infiltration of immune cells, such as CD8+ T cells and Tregs; higher cancer-associated fibroblast (CAF) infiltration; lower immune function; and lower checkpoint expression. The signature can also be applied to distinguish whether patients benefit from immunotherapy. In addition, the high-risk group had a higher TMB and more gene mutations, including those in TP53, CSMD1, CDKN2A, and MUC17.Conclusion: We propose a prognostic signature based on glycosyltransferases for HNSCC patients that may provide potential targets and biomarkers for the precise treatment of HNSCC.https://www.frontiersin.org/articles/10.3389/fgene.2022.856671/fullglycosyltransferaseprognostic signaturehead and neck squamous cell carcinomaimmune cell infiltrationtumor mutational burden |
| spellingShingle | Huili Wu Huili Wu Huili Wu Huili Wu Xiao Zhao Xiao Zhao Xiao Zhao Xiao Zhao Tingting Zhu Tingting Zhu Tingting Zhu Tingting Zhu Di Rong Di Rong Di Rong Di Rong Ying Wang Ying Wang Ying Wang Ying Wang Diya Leng Diya Leng Diya Leng Diya Leng Daming Wu Daming Wu Daming Wu Daming Wu A Glycosyltransferase-Related Signature for Predicting Overall Survival in Head and Neck Squamous Cell Carcinoma Frontiers in Genetics glycosyltransferase prognostic signature head and neck squamous cell carcinoma immune cell infiltration tumor mutational burden |
| title | A Glycosyltransferase-Related Signature for Predicting Overall Survival in Head and Neck Squamous Cell Carcinoma |
| title_full | A Glycosyltransferase-Related Signature for Predicting Overall Survival in Head and Neck Squamous Cell Carcinoma |
| title_fullStr | A Glycosyltransferase-Related Signature for Predicting Overall Survival in Head and Neck Squamous Cell Carcinoma |
| title_full_unstemmed | A Glycosyltransferase-Related Signature for Predicting Overall Survival in Head and Neck Squamous Cell Carcinoma |
| title_short | A Glycosyltransferase-Related Signature for Predicting Overall Survival in Head and Neck Squamous Cell Carcinoma |
| title_sort | glycosyltransferase related signature for predicting overall survival in head and neck squamous cell carcinoma |
| topic | glycosyltransferase prognostic signature head and neck squamous cell carcinoma immune cell infiltration tumor mutational burden |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2022.856671/full |
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