<i>Pochonia chlamydosporia</i> Isolate PC-170-Induced Expression of Marker Genes for Defense Pathways in Tomatoes Challenged by Different Pathogens
<i>Pochonia chlamydosporia</i> is a fungal parasite of nematode eggs. Studies have shown that some strains of <i>Pochonia chlamydosporia</i> can promote plant growth and induce plants’ systemic resistance to root-knot nematodes by colonizing in their roots. This study aimed t...
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MDPI AG
2021-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/9/1882 |
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| author | Xia Zhuang Jian-Long Zhao Miao Bai Xing-Xing Ping Yan-Lin Li Yu-Hong Yang Zhen-Chuan Mao Guo-Shun Yang Bing-Yan Xie |
| author_facet | Xia Zhuang Jian-Long Zhao Miao Bai Xing-Xing Ping Yan-Lin Li Yu-Hong Yang Zhen-Chuan Mao Guo-Shun Yang Bing-Yan Xie |
| author_sort | Xia Zhuang |
| collection | DOAJ |
| description | <i>Pochonia chlamydosporia</i> is a fungal parasite of nematode eggs. Studies have shown that some strains of <i>Pochonia chlamydosporia</i> can promote plant growth and induce plants’ systemic resistance to root-knot nematodes by colonizing in their roots. This study aimed to verify the effect of the PC-170 strain on tomato growth and systemic resistance. Split-root experiments were conducted to observe the systemic resistance induced by PC-170. To explore the defense pathway that was excited due to the colonization by PC-170, we tested the expression of marker genes for defense pathways, and used mutant lines to verify the role of plant defense pathways. Our results showed that PC-170 can colonize roots, and promotes growth. We found a role for jasmonic acid (JA) in modulating tomato colonization by PC-170. PC-170 can activate tomato defense responses to reduce susceptibility to infection by the root-knot nematode <i>Meloidogyne incognita</i>, and induced resistance to some pathogens in tomatoes. The marker genes of the defense pathway were significantly induced after PC-170 colonization. However, salicylic acid (SA)- and jasmonic acid (JA)-dependent defenses in roots were variable with the invasion of different pathogens. Defense pathways play different roles at different points in time. SA- and JA-dependent defense pathways were shown to cross-communicate. Different phytohormones have been involved in tomato plants’ responses against different pathogens. Our study confirmed that adaptive JA signaling is necessary to regulate PC-170 colonization and induce systemic resistance in tomatoes. |
| first_indexed | 2024-03-10T07:25:40Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T07:25:40Z |
| publishDate | 2021-09-01 |
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| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-fb0ab0806a1c4bca860fdc44bb743bd32023-11-22T14:18:32ZengMDPI AGMicroorganisms2076-26072021-09-0199188210.3390/microorganisms9091882<i>Pochonia chlamydosporia</i> Isolate PC-170-Induced Expression of Marker Genes for Defense Pathways in Tomatoes Challenged by Different PathogensXia Zhuang0Jian-Long Zhao1Miao Bai2Xing-Xing Ping3Yan-Lin Li4Yu-Hong Yang5Zhen-Chuan Mao6Guo-Shun Yang7Bing-Yan Xie8Engineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding of Ministry of Education, College of Horticulture, Hunan Agricultural University, Changsha 410128, ChinaThe Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaEngineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding of Ministry of Education, College of Horticulture, Hunan Agricultural University, Changsha 410128, ChinaThe Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaEngineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding of Ministry of Education, College of Horticulture, Hunan Agricultural University, Changsha 410128, ChinaThe Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaThe Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaEngineering Research Center for Horticultural Crop Germplasm Creation and New Variety Breeding of Ministry of Education, College of Horticulture, Hunan Agricultural University, Changsha 410128, ChinaThe Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China<i>Pochonia chlamydosporia</i> is a fungal parasite of nematode eggs. Studies have shown that some strains of <i>Pochonia chlamydosporia</i> can promote plant growth and induce plants’ systemic resistance to root-knot nematodes by colonizing in their roots. This study aimed to verify the effect of the PC-170 strain on tomato growth and systemic resistance. Split-root experiments were conducted to observe the systemic resistance induced by PC-170. To explore the defense pathway that was excited due to the colonization by PC-170, we tested the expression of marker genes for defense pathways, and used mutant lines to verify the role of plant defense pathways. Our results showed that PC-170 can colonize roots, and promotes growth. We found a role for jasmonic acid (JA) in modulating tomato colonization by PC-170. PC-170 can activate tomato defense responses to reduce susceptibility to infection by the root-knot nematode <i>Meloidogyne incognita</i>, and induced resistance to some pathogens in tomatoes. The marker genes of the defense pathway were significantly induced after PC-170 colonization. However, salicylic acid (SA)- and jasmonic acid (JA)-dependent defenses in roots were variable with the invasion of different pathogens. Defense pathways play different roles at different points in time. SA- and JA-dependent defense pathways were shown to cross-communicate. Different phytohormones have been involved in tomato plants’ responses against different pathogens. Our study confirmed that adaptive JA signaling is necessary to regulate PC-170 colonization and induce systemic resistance in tomatoes.https://www.mdpi.com/2076-2607/9/9/1882induced defensesjasmonic acid (JA)<i>Pochonia chlamydosporia</i>pathogenroot endophytessalicylic acid (SA) |
| spellingShingle | Xia Zhuang Jian-Long Zhao Miao Bai Xing-Xing Ping Yan-Lin Li Yu-Hong Yang Zhen-Chuan Mao Guo-Shun Yang Bing-Yan Xie <i>Pochonia chlamydosporia</i> Isolate PC-170-Induced Expression of Marker Genes for Defense Pathways in Tomatoes Challenged by Different Pathogens Microorganisms induced defenses jasmonic acid (JA) <i>Pochonia chlamydosporia</i> pathogen root endophytes salicylic acid (SA) |
| title | <i>Pochonia chlamydosporia</i> Isolate PC-170-Induced Expression of Marker Genes for Defense Pathways in Tomatoes Challenged by Different Pathogens |
| title_full | <i>Pochonia chlamydosporia</i> Isolate PC-170-Induced Expression of Marker Genes for Defense Pathways in Tomatoes Challenged by Different Pathogens |
| title_fullStr | <i>Pochonia chlamydosporia</i> Isolate PC-170-Induced Expression of Marker Genes for Defense Pathways in Tomatoes Challenged by Different Pathogens |
| title_full_unstemmed | <i>Pochonia chlamydosporia</i> Isolate PC-170-Induced Expression of Marker Genes for Defense Pathways in Tomatoes Challenged by Different Pathogens |
| title_short | <i>Pochonia chlamydosporia</i> Isolate PC-170-Induced Expression of Marker Genes for Defense Pathways in Tomatoes Challenged by Different Pathogens |
| title_sort | i pochonia chlamydosporia i isolate pc 170 induced expression of marker genes for defense pathways in tomatoes challenged by different pathogens |
| topic | induced defenses jasmonic acid (JA) <i>Pochonia chlamydosporia</i> pathogen root endophytes salicylic acid (SA) |
| url | https://www.mdpi.com/2076-2607/9/9/1882 |
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