Study on mutagenesis of high protease producing thermophilic bacteria by UV-DES and hydrolysis of excess sludge
Excess sludge a resource-rich organic waste, can be solubilized by thermophilic enzymes to extract proteins and anaerobic digestion of methane production to reduce sludge and use resources. At the same time, it can improve new ideas to relieve the pressure of the energy industry. To solve the proble...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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EDP Sciences
2023-01-01
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| Series: | E3S Web of Conferences |
| Online Access: | https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/103/e3sconf_icaeer2023_01013.pdf |
| _version_ | 1827373107374981120 |
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| author | Wang Changwei Ning Zhifang Zhong Weizhang Li Yanfei Hou Jiwei Wang Zhaoyang Zhang Haoran Dong Xilei |
| author_facet | Wang Changwei Ning Zhifang Zhong Weizhang Li Yanfei Hou Jiwei Wang Zhaoyang Zhang Haoran Dong Xilei |
| author_sort | Wang Changwei |
| collection | DOAJ |
| description | Excess sludge a resource-rich organic waste, can be solubilized by thermophilic enzymes to extract proteins and anaerobic digestion of methane production to reduce sludge and use resources. At the same time, it can improve new ideas to relieve the pressure of the energy industry. To solve the problems of low enzyme-producing activity of wild strains and low hydrolysis rate of ES, this study utilized UV-DES compound mutagenesis to screen dominant mutant strains under optimized conditions, which were added to ES hydrolysis reaction system. The results showed that UV-DES mutagenesis could effectively improve the ability of S-TE and promote the hydrolysis of ES. The protease activity of the mutant strain KT16 obtained by screening was 46.7% higher than that of the original strain DC8. KT16 effectively degraded insoluble organic matter, reduced the average particle size of ES, and increased the concentrations of extracellular proteins, polypeptides, and amino acids to 1731.8, 521.3, and 510 mg·L-1, respectively. This study will provide a reference for the resource utilized. |
| first_indexed | 2024-03-08T11:11:57Z |
| format | Article |
| id | doaj.art-fb0ac526966241ea8205dd57ad1bb642 |
| institution | Directory Open Access Journal |
| issn | 2267-1242 |
| language | English |
| last_indexed | 2024-03-08T11:11:57Z |
| publishDate | 2023-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | E3S Web of Conferences |
| spelling | doaj.art-fb0ac526966241ea8205dd57ad1bb6422024-01-26T10:43:49ZengEDP SciencesE3S Web of Conferences2267-12422023-01-014660101310.1051/e3sconf/202346601013e3sconf_icaeer2023_01013Study on mutagenesis of high protease producing thermophilic bacteria by UV-DES and hydrolysis of excess sludgeWang Changwei0Ning Zhifang1Zhong Weizhang2Li Yanfei3Hou Jiwei4Wang Zhaoyang5Zhang Haoran6Dong Xilei7School of Environmental Science and Engineering, Hebei University of Science and TechnologySchool of Environmental Science and Engineering, Hebei University of Science and TechnologySchool of Environmental Science and Engineering, Hebei University of Science and TechnologySchool of Environmental Science and Engineering, Hebei University of Science and TechnologySchool of Environmental Science and Engineering, Hebei University of Science and TechnologySchool of Environmental Science and Engineering, Hebei University of Science and TechnologySchool of Environmental Science and Engineering, Hebei University of Science and TechnologySchool of Environmental Science and Engineering, Hebei University of Science and TechnologyExcess sludge a resource-rich organic waste, can be solubilized by thermophilic enzymes to extract proteins and anaerobic digestion of methane production to reduce sludge and use resources. At the same time, it can improve new ideas to relieve the pressure of the energy industry. To solve the problems of low enzyme-producing activity of wild strains and low hydrolysis rate of ES, this study utilized UV-DES compound mutagenesis to screen dominant mutant strains under optimized conditions, which were added to ES hydrolysis reaction system. The results showed that UV-DES mutagenesis could effectively improve the ability of S-TE and promote the hydrolysis of ES. The protease activity of the mutant strain KT16 obtained by screening was 46.7% higher than that of the original strain DC8. KT16 effectively degraded insoluble organic matter, reduced the average particle size of ES, and increased the concentrations of extracellular proteins, polypeptides, and amino acids to 1731.8, 521.3, and 510 mg·L-1, respectively. This study will provide a reference for the resource utilized.https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/103/e3sconf_icaeer2023_01013.pdf |
| spellingShingle | Wang Changwei Ning Zhifang Zhong Weizhang Li Yanfei Hou Jiwei Wang Zhaoyang Zhang Haoran Dong Xilei Study on mutagenesis of high protease producing thermophilic bacteria by UV-DES and hydrolysis of excess sludge E3S Web of Conferences |
| title | Study on mutagenesis of high protease producing thermophilic bacteria by UV-DES and hydrolysis of excess sludge |
| title_full | Study on mutagenesis of high protease producing thermophilic bacteria by UV-DES and hydrolysis of excess sludge |
| title_fullStr | Study on mutagenesis of high protease producing thermophilic bacteria by UV-DES and hydrolysis of excess sludge |
| title_full_unstemmed | Study on mutagenesis of high protease producing thermophilic bacteria by UV-DES and hydrolysis of excess sludge |
| title_short | Study on mutagenesis of high protease producing thermophilic bacteria by UV-DES and hydrolysis of excess sludge |
| title_sort | study on mutagenesis of high protease producing thermophilic bacteria by uv des and hydrolysis of excess sludge |
| url | https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/103/e3sconf_icaeer2023_01013.pdf |
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