Efficacy and safety profile of a subtilisin protease produced by fermentation in Bacillus licheniformis to be used as a feed additive
The efficacy and safety of a novel Subtilisin protease from a Bacillus sp. produced in Bacillus licheniformis was investigated in broiler chickens, and a range of toxicological tests, respectively. The B. licheniformis production strain culture supernatant was not found cytotoxic in a Vero cell assa...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-08-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844022013184 |
| _version_ | 1811188058301661184 |
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| author | Denisa Cupi Michael Thorsen Signe Gry Elvig-Jørgensen Linda Wulf-Andersen Jose-Otavio Berti-Sorbara Aaron J. Cowieson Murtala Umar Faruk |
| author_facet | Denisa Cupi Michael Thorsen Signe Gry Elvig-Jørgensen Linda Wulf-Andersen Jose-Otavio Berti-Sorbara Aaron J. Cowieson Murtala Umar Faruk |
| author_sort | Denisa Cupi |
| collection | DOAJ |
| description | The efficacy and safety of a novel Subtilisin protease from a Bacillus sp. produced in Bacillus licheniformis was investigated in broiler chickens, and a range of toxicological tests, respectively. The B. licheniformis production strain culture supernatant was not found cytotoxic in a Vero cell assay. Subtilisin was non-mutagenic and non-clastogenic in in-vitro tests, and did not exhibit irritating potential to the eye or skin in ex-vivo/in-vitro models. Oral administration of Subtilisin to rats did not cause any adverse effects in a 13-week sub-chronic toxicity study. In addition, a 35-day dose response broiler performance trial conducted with Subtilisin (30,000 and 60,000 NFP/kg diet), showed a significant linear improvement in both body weight gain and feed conversion ratio up to 35 days of protease supplementation.In conclusion, there are no safety concerns using this novel Subtilisin as a feed additive, and the protease is efficient in improving broiler growth performance, making it a good candidate for use as a feed additive. |
| first_indexed | 2024-04-11T14:13:05Z |
| format | Article |
| id | doaj.art-fb0c6c0c28694e13b5131f43e8473038 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-11T14:13:05Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-fb0c6c0c28694e13b5131f43e84730382022-12-22T04:19:37ZengElsevierHeliyon2405-84402022-08-0188e10030Efficacy and safety profile of a subtilisin protease produced by fermentation in Bacillus licheniformis to be used as a feed additiveDenisa Cupi0Michael Thorsen1Signe Gry Elvig-Jørgensen2Linda Wulf-Andersen3Jose-Otavio Berti-Sorbara4Aaron J. Cowieson5Murtala Umar Faruk6Novozymes A/S, Krogshoejvej 36, DK-2880 Bagsvaerd, Denmark; Corresponding author.Novozymes A/S, Krogshoejvej 36, DK-2880 Bagsvaerd, DenmarkNovozymes A/S, Krogshoejvej 36, DK-2880 Bagsvaerd, DenmarkNovozymes A/S, Krogshoejvej 36, DK-2880 Bagsvaerd, DenmarkDSM Nutritional Products Ltd, CH-4002 Basel, SwitzerlandDSM Nutritional Products Ltd, CH-4002 Basel, SwitzerlandDSM Nutritional Products France, F-68128 Village-Neuf, FranceThe efficacy and safety of a novel Subtilisin protease from a Bacillus sp. produced in Bacillus licheniformis was investigated in broiler chickens, and a range of toxicological tests, respectively. The B. licheniformis production strain culture supernatant was not found cytotoxic in a Vero cell assay. Subtilisin was non-mutagenic and non-clastogenic in in-vitro tests, and did not exhibit irritating potential to the eye or skin in ex-vivo/in-vitro models. Oral administration of Subtilisin to rats did not cause any adverse effects in a 13-week sub-chronic toxicity study. In addition, a 35-day dose response broiler performance trial conducted with Subtilisin (30,000 and 60,000 NFP/kg diet), showed a significant linear improvement in both body weight gain and feed conversion ratio up to 35 days of protease supplementation.In conclusion, there are no safety concerns using this novel Subtilisin as a feed additive, and the protease is efficient in improving broiler growth performance, making it a good candidate for use as a feed additive.http://www.sciencedirect.com/science/article/pii/S2405844022013184SubtilisinBacillus licheniformisToxicitySafetyFeed additiveBroiler chickens |
| spellingShingle | Denisa Cupi Michael Thorsen Signe Gry Elvig-Jørgensen Linda Wulf-Andersen Jose-Otavio Berti-Sorbara Aaron J. Cowieson Murtala Umar Faruk Efficacy and safety profile of a subtilisin protease produced by fermentation in Bacillus licheniformis to be used as a feed additive Heliyon Subtilisin Bacillus licheniformis Toxicity Safety Feed additive Broiler chickens |
| title | Efficacy and safety profile of a subtilisin protease produced by fermentation in Bacillus licheniformis to be used as a feed additive |
| title_full | Efficacy and safety profile of a subtilisin protease produced by fermentation in Bacillus licheniformis to be used as a feed additive |
| title_fullStr | Efficacy and safety profile of a subtilisin protease produced by fermentation in Bacillus licheniformis to be used as a feed additive |
| title_full_unstemmed | Efficacy and safety profile of a subtilisin protease produced by fermentation in Bacillus licheniformis to be used as a feed additive |
| title_short | Efficacy and safety profile of a subtilisin protease produced by fermentation in Bacillus licheniformis to be used as a feed additive |
| title_sort | efficacy and safety profile of a subtilisin protease produced by fermentation in bacillus licheniformis to be used as a feed additive |
| topic | Subtilisin Bacillus licheniformis Toxicity Safety Feed additive Broiler chickens |
| url | http://www.sciencedirect.com/science/article/pii/S2405844022013184 |
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