Lhx1 in the proximal region of the optic vesicle permits neural retina development in the chicken
Summary How the eye forms has been one of the fundamental issues in developmental biology. The retinal anlage first appears as the optic vesicle (OV) evaginating from the forebrain. Subsequently, its distal portion invaginates to form the two-walled optic cup, which develops into the outer pigmented...
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| Format: | Article |
| Language: | English |
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The Company of Biologists
2012-08-01
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| Series: | Biology Open |
| Subjects: | |
| Online Access: | http://bio.biologists.org/content/1/11/1083 |
| _version_ | 1818740218400866304 |
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| author | Takumi Kawaue Mayumi Okamoto Akane Matsuyo Junji Inoue Yuhki Ueda Sayuri Tomonari Sumihare Noji Hideyo Ohuchi |
| author_facet | Takumi Kawaue Mayumi Okamoto Akane Matsuyo Junji Inoue Yuhki Ueda Sayuri Tomonari Sumihare Noji Hideyo Ohuchi |
| author_sort | Takumi Kawaue |
| collection | DOAJ |
| description | Summary
How the eye forms has been one of the fundamental issues in developmental biology. The retinal anlage first appears as the optic vesicle (OV) evaginating from the forebrain. Subsequently, its distal portion invaginates to form the two-walled optic cup, which develops into the outer pigmented and inner neurosensory layers of the retina. Recent work has shown that this optic-cup morphogenesis proceeds as a self-organizing activity without any extrinsic molecules. However, intrinsic factors that regulate this process have not been elucidated. Here we show that a LIM-homeobox gene, Lhx1, normally expressed in the proximal region of the nascent OV, induces a second neurosensory retina formation from the outer pigmented retina when overexpressed in the chicken OV. Lhx2, another LIM-homeobox gene supposed to be involved in early OV formation, could not substitute this function of Lhx1, while Lhx5, closely related to Lhx1, could replace it. Conversely, knockdown of Lhx1 expression by RNA interference resulted in the formation of a small or pigmented vesicle. These results suggest that the proximal region demarcated by Lhx1 expression permits OV development, eventually dividing the two retinal domains. |
| first_indexed | 2024-12-18T01:37:14Z |
| format | Article |
| id | doaj.art-fb0f448e8d3c44289a1d3d44149bc0d1 |
| institution | Directory Open Access Journal |
| issn | 2046-6390 |
| language | English |
| last_indexed | 2024-12-18T01:37:14Z |
| publishDate | 2012-08-01 |
| publisher | The Company of Biologists |
| record_format | Article |
| series | Biology Open |
| spelling | doaj.art-fb0f448e8d3c44289a1d3d44149bc0d12022-12-21T21:25:25ZengThe Company of BiologistsBiology Open2046-63902012-08-011111083109310.1242/bio.2012139620121396Lhx1 in the proximal region of the optic vesicle permits neural retina development in the chickenTakumi Kawaue0Mayumi Okamoto1Akane Matsuyo2Junji Inoue3Yuhki Ueda4Sayuri Tomonari5Sumihare Noji6Hideyo Ohuchi7 Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School, 2-1 Minami-Josanjima-cho, Tokushima 770-8506, Japan Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School, 2-1 Minami-Josanjima-cho, Tokushima 770-8506, Japan Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School, 2-1 Minami-Josanjima-cho, Tokushima 770-8506, Japan Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School, 2-1 Minami-Josanjima-cho, Tokushima 770-8506, Japan Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School, 2-1 Minami-Josanjima-cho, Tokushima 770-8506, Japan Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School, 2-1 Minami-Josanjima-cho, Tokushima 770-8506, Japan Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School, 2-1 Minami-Josanjima-cho, Tokushima 770-8506, Japan Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School, 2-1 Minami-Josanjima-cho, Tokushima 770-8506, Japan Summary How the eye forms has been one of the fundamental issues in developmental biology. The retinal anlage first appears as the optic vesicle (OV) evaginating from the forebrain. Subsequently, its distal portion invaginates to form the two-walled optic cup, which develops into the outer pigmented and inner neurosensory layers of the retina. Recent work has shown that this optic-cup morphogenesis proceeds as a self-organizing activity without any extrinsic molecules. However, intrinsic factors that regulate this process have not been elucidated. Here we show that a LIM-homeobox gene, Lhx1, normally expressed in the proximal region of the nascent OV, induces a second neurosensory retina formation from the outer pigmented retina when overexpressed in the chicken OV. Lhx2, another LIM-homeobox gene supposed to be involved in early OV formation, could not substitute this function of Lhx1, while Lhx5, closely related to Lhx1, could replace it. Conversely, knockdown of Lhx1 expression by RNA interference resulted in the formation of a small or pigmented vesicle. These results suggest that the proximal region demarcated by Lhx1 expression permits OV development, eventually dividing the two retinal domains.http://bio.biologists.org/content/1/11/1083Lhx1Lhx5Optic vesicleLIM-homeoboxChickenEye developmentNeural retina |
| spellingShingle | Takumi Kawaue Mayumi Okamoto Akane Matsuyo Junji Inoue Yuhki Ueda Sayuri Tomonari Sumihare Noji Hideyo Ohuchi Lhx1 in the proximal region of the optic vesicle permits neural retina development in the chicken Biology Open Lhx1 Lhx5 Optic vesicle LIM-homeobox Chicken Eye development Neural retina |
| title | Lhx1 in the proximal region of the optic vesicle permits neural retina development in the chicken |
| title_full | Lhx1 in the proximal region of the optic vesicle permits neural retina development in the chicken |
| title_fullStr | Lhx1 in the proximal region of the optic vesicle permits neural retina development in the chicken |
| title_full_unstemmed | Lhx1 in the proximal region of the optic vesicle permits neural retina development in the chicken |
| title_short | Lhx1 in the proximal region of the optic vesicle permits neural retina development in the chicken |
| title_sort | lhx1 in the proximal region of the optic vesicle permits neural retina development in the chicken |
| topic | Lhx1 Lhx5 Optic vesicle LIM-homeobox Chicken Eye development Neural retina |
| url | http://bio.biologists.org/content/1/11/1083 |
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