Differential Immunoexpression of BRAF/V600E, Senescence Markers, PTEN, and T-type Calcium Channels in Acquired Naevi According to their Histopathological and Dermoscopic Classification
BRAF/V600E mutation and other cell growth/growth-control mechanisms are involved in naevogenesis and melanomagenesis. Immunoexpression of BRAF/V600E and other molecules (p16, phosphatase and tensin homologue (PTEN), Ki67, hTERT and Cav3.1 and 3.2 calcium channels) were investigated in 80 histopatho...
| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Medical Journals Sweden
2021-11-01
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| Series: | Acta Dermato-Venereologica |
| Subjects: | |
| Online Access: | https://medicaljournalssweden.se/actadv/article/view/361 |
| _version_ | 1831687449878724608 |
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| author | Sara Moreno Oscar Maiques Carla Barcelo Marta Romero Maria Santacana Ignacio Gómez Dolors Cuevas Ana Velasco Alvar Vea Anna Macia Ramon Boix Joan Valls Sonia Gatius Carles Canti Xavier Matias-Guiu Xavier Soria Rosa M. Marti |
| author_facet | Sara Moreno Oscar Maiques Carla Barcelo Marta Romero Maria Santacana Ignacio Gómez Dolors Cuevas Ana Velasco Alvar Vea Anna Macia Ramon Boix Joan Valls Sonia Gatius Carles Canti Xavier Matias-Guiu Xavier Soria Rosa M. Marti |
| author_sort | Sara Moreno |
| collection | DOAJ |
| description | BRAF/V600E mutation and other cell growth/growth-control mechanisms are involved in naevogenesis and melanomagenesis. Immunoexpression of BRAF/V600E and other molecules (p16, phosphatase and tensin homologue (PTEN), Ki67, hTERT and Cav3.1 and 3.2 calcium channels) were investigated in 80 histopathologically and dermoscopically classified acquired naevi. Regarding BRAF/V600E, dysplastic naevi showed lower immunostaining than common naevi, which was significant in comparison with intradermal naevi, which showed the highest BRAF/V600E histoscore. Junctional naevi showed the lowest BRAF/V600E levels. Globular/cobblestone and reticular dermoscopic patterns were consistently associated with high and low BRAF/V600E immunoexpression, respectively, but Zalaudek’s peripheral globule pattern (CR/PG) showed the highest BRAF/V600E immunoexpression. Among global patterns, the previously not investigated multicomponent pattern showed the lowest BRAF/V600E immunoexpression. Regarding the remaining biomarkers, new immunohistochemical features were found, in particular p16 and PTEN low expression in multicomponent pattern; and Ki67, hTERT and Cav.3.1 high expression in CR/PG. In conclusion, histopathology and dermoscopy provide complementary information regarding the biology of melanocytic naevi. |
| first_indexed | 2024-12-20T08:58:40Z |
| format | Article |
| id | doaj.art-fb10f1cd9c824c5b98cd290cac95a850 |
| institution | Directory Open Access Journal |
| issn | 0001-5555 1651-2057 |
| language | English |
| last_indexed | 2024-12-20T08:58:40Z |
| publishDate | 2021-11-01 |
| publisher | Medical Journals Sweden |
| record_format | Article |
| series | Acta Dermato-Venereologica |
| spelling | doaj.art-fb10f1cd9c824c5b98cd290cac95a8502022-12-21T19:45:56ZengMedical Journals SwedenActa Dermato-Venereologica0001-55551651-20572021-11-011011110.2340/actadv.v101.361Differential Immunoexpression of BRAF/V600E, Senescence Markers, PTEN, and T-type Calcium Channels in Acquired Naevi According to their Histopathological and Dermoscopic ClassificationSara Moreno0Oscar MaiquesCarla BarceloMarta RomeroMaria SantacanaIgnacio GómezDolors CuevasAna VelascoAlvar VeaAnna MaciaRamon BoixJoan VallsSonia GatiusCarles CantiXavier Matias-GuiuXavier SoriaRosa M. MartiDepartment of Dermatology, Hospital Universitari Arnau de Vilanova, University of Lleida, IRBLleida, Avda Alcalde Rovira Roure 80, ES-25198 Lleida, SpainBRAF/V600E mutation and other cell growth/growth-control mechanisms are involved in naevogenesis and melanomagenesis. Immunoexpression of BRAF/V600E and other molecules (p16, phosphatase and tensin homologue (PTEN), Ki67, hTERT and Cav3.1 and 3.2 calcium channels) were investigated in 80 histopathologically and dermoscopically classified acquired naevi. Regarding BRAF/V600E, dysplastic naevi showed lower immunostaining than common naevi, which was significant in comparison with intradermal naevi, which showed the highest BRAF/V600E histoscore. Junctional naevi showed the lowest BRAF/V600E levels. Globular/cobblestone and reticular dermoscopic patterns were consistently associated with high and low BRAF/V600E immunoexpression, respectively, but Zalaudek’s peripheral globule pattern (CR/PG) showed the highest BRAF/V600E immunoexpression. Among global patterns, the previously not investigated multicomponent pattern showed the lowest BRAF/V600E immunoexpression. Regarding the remaining biomarkers, new immunohistochemical features were found, in particular p16 and PTEN low expression in multicomponent pattern; and Ki67, hTERT and Cav.3.1 high expression in CR/PG. In conclusion, histopathology and dermoscopy provide complementary information regarding the biology of melanocytic naevi.https://medicaljournalssweden.se/actadv/article/view/361acquired melanocytic naevusBRAF-V600Esenescence markersPTENT-type calcium channels |
| spellingShingle | Sara Moreno Oscar Maiques Carla Barcelo Marta Romero Maria Santacana Ignacio Gómez Dolors Cuevas Ana Velasco Alvar Vea Anna Macia Ramon Boix Joan Valls Sonia Gatius Carles Canti Xavier Matias-Guiu Xavier Soria Rosa M. Marti Differential Immunoexpression of BRAF/V600E, Senescence Markers, PTEN, and T-type Calcium Channels in Acquired Naevi According to their Histopathological and Dermoscopic Classification Acta Dermato-Venereologica acquired melanocytic naevus BRAF-V600E senescence markers PTEN T-type calcium channels |
| title | Differential Immunoexpression of BRAF/V600E, Senescence Markers, PTEN, and T-type Calcium Channels in Acquired Naevi According to their Histopathological and Dermoscopic Classification |
| title_full | Differential Immunoexpression of BRAF/V600E, Senescence Markers, PTEN, and T-type Calcium Channels in Acquired Naevi According to their Histopathological and Dermoscopic Classification |
| title_fullStr | Differential Immunoexpression of BRAF/V600E, Senescence Markers, PTEN, and T-type Calcium Channels in Acquired Naevi According to their Histopathological and Dermoscopic Classification |
| title_full_unstemmed | Differential Immunoexpression of BRAF/V600E, Senescence Markers, PTEN, and T-type Calcium Channels in Acquired Naevi According to their Histopathological and Dermoscopic Classification |
| title_short | Differential Immunoexpression of BRAF/V600E, Senescence Markers, PTEN, and T-type Calcium Channels in Acquired Naevi According to their Histopathological and Dermoscopic Classification |
| title_sort | differential immunoexpression of braf v600e senescence markers pten and t type calcium channels in acquired naevi according to their histopathological and dermoscopic classification |
| topic | acquired melanocytic naevus BRAF-V600E senescence markers PTEN T-type calcium channels |
| url | https://medicaljournalssweden.se/actadv/article/view/361 |
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