Reproducibility of Densitometric and Biomechanical Assessment of the Mouse Tibia From In Vivo Micro-CT Images
Interventions for bone diseases (e.g. osteoporosis) require testing in animal models before clinical translation and the mouse tibia is among the most common tested anatomical sites. In vivo micro-Computed Tomography (microCT) based measurements of the geometrical and densitometric properties are no...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-06-01
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| Series: | Frontiers in Endocrinology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fendo.2022.915938/full |
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| author | Sara Oliviero Sara Oliviero Sara Oliviero Sara Oliviero Vee San Cheong Vee San Cheong Bryant C. Roberts Bryant C. Roberts Carlos Amnael Orozco Diaz William Griffiths Ilaria Bellantuono Ilaria Bellantuono Ilaria Bellantuono Enrico Dall’Ara Enrico Dall’Ara Enrico Dall’Ara |
| author_facet | Sara Oliviero Sara Oliviero Sara Oliviero Sara Oliviero Vee San Cheong Vee San Cheong Bryant C. Roberts Bryant C. Roberts Carlos Amnael Orozco Diaz William Griffiths Ilaria Bellantuono Ilaria Bellantuono Ilaria Bellantuono Enrico Dall’Ara Enrico Dall’Ara Enrico Dall’Ara |
| author_sort | Sara Oliviero |
| collection | DOAJ |
| description | Interventions for bone diseases (e.g. osteoporosis) require testing in animal models before clinical translation and the mouse tibia is among the most common tested anatomical sites. In vivo micro-Computed Tomography (microCT) based measurements of the geometrical and densitometric properties are non-invasive and therefore constitute an important tool in preclinical studies. Moreover, validated micro-Finite Element (microFE) models can be used for predicting the bone mechanical properties non-invasively. However, considering that the image processing pipeline requires operator-dependant steps, the reproducibility of these measurements has to be assessed. The aim of this study was to evaluate the intra- and inter-operator reproducibility of several bone parameters measured from microCT images. Ten in vivo microCT images of the right tibia of five mice (at 18 and 22 weeks of age) were processed. One experienced operator (intra-operator analysis) and three different operators (inter-operator) aligned each image to a reference through a rigid registration and selected a volume of interest below the growth plate. From each image the following parameters were measured: total bone mineral content (BMC) and density (BMD), BMC in 40 subregions (ten longitudinal sections, four quadrants), microFE-based stiffness and failure load. Intra-operator reproducibility was acceptable for all parameters (precision error, PE < 3.71%), with lowest reproducibility for stiffness (3.06% at week 18, 3.71% at week 22). The inter-operator reproducibility was slightly lower (PE < 4.25%), although still acceptable for assessing the properties of most interventions. The lowest reproducibility was found for BMC in the lateral sector at the midshaft (PE = 4.25%). Densitometric parameters were more reproducible than most standard morphometric parameters calculated in the proximal trabecular bone. In conclusion, microCT and microFE models provide reproducible measurements for non-invasive assessment of the mouse tibia properties. |
| first_indexed | 2024-12-12T07:57:16Z |
| format | Article |
| id | doaj.art-fb12529e69f141a382f1439663f055cc |
| institution | Directory Open Access Journal |
| issn | 1664-2392 |
| language | English |
| last_indexed | 2024-12-12T07:57:16Z |
| publishDate | 2022-06-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Endocrinology |
| spelling | doaj.art-fb12529e69f141a382f1439663f055cc2022-12-22T00:32:17ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922022-06-011310.3389/fendo.2022.915938915938Reproducibility of Densitometric and Biomechanical Assessment of the Mouse Tibia From In Vivo Micro-CT ImagesSara Oliviero0Sara Oliviero1Sara Oliviero2Sara Oliviero3Vee San Cheong4Vee San Cheong5Bryant C. Roberts6Bryant C. Roberts7Carlos Amnael Orozco Diaz8William Griffiths9Ilaria Bellantuono10Ilaria Bellantuono11Ilaria Bellantuono12Enrico Dall’Ara13Enrico Dall’Ara14Enrico Dall’Ara15Department of Oncology and Metabolism, Mellanby Centre for bone Research, University of Sheffield, Sheffield, United KingdomINSIGNEO Institute for In Silico Medicine, University of Sheffield, Sheffield, United KingdomDepartment of Industrial Engineering, Alma Mater Studiorum, University of Bologna, Bologna, ItalyMedical Technology Lab, IRCCS Istituto Ortopedico Rizzoli, Bologna, ItalyINSIGNEO Institute for In Silico Medicine, University of Sheffield, Sheffield, United KingdomDepartment of Automatic Control and Systems Engineering, University of Sheffield, Sheffield, United KingdomDepartment of Oncology and Metabolism, Mellanby Centre for bone Research, University of Sheffield, Sheffield, United KingdomINSIGNEO Institute for In Silico Medicine, University of Sheffield, Sheffield, United KingdomDepartment of Oncology and Metabolism, Mellanby Centre for bone Research, University of Sheffield, Sheffield, United KingdomINSIGNEO Institute for In Silico Medicine, University of Sheffield, Sheffield, United KingdomDepartment of Oncology and Metabolism, Mellanby Centre for bone Research, University of Sheffield, Sheffield, United KingdomINSIGNEO Institute for In Silico Medicine, University of Sheffield, Sheffield, United KingdomHealthy Lifespan Institute, University of Sheffield, Sheffield, United KingdomDepartment of Oncology and Metabolism, Mellanby Centre for bone Research, University of Sheffield, Sheffield, United KingdomINSIGNEO Institute for In Silico Medicine, University of Sheffield, Sheffield, United KingdomHealthy Lifespan Institute, University of Sheffield, Sheffield, United KingdomInterventions for bone diseases (e.g. osteoporosis) require testing in animal models before clinical translation and the mouse tibia is among the most common tested anatomical sites. In vivo micro-Computed Tomography (microCT) based measurements of the geometrical and densitometric properties are non-invasive and therefore constitute an important tool in preclinical studies. Moreover, validated micro-Finite Element (microFE) models can be used for predicting the bone mechanical properties non-invasively. However, considering that the image processing pipeline requires operator-dependant steps, the reproducibility of these measurements has to be assessed. The aim of this study was to evaluate the intra- and inter-operator reproducibility of several bone parameters measured from microCT images. Ten in vivo microCT images of the right tibia of five mice (at 18 and 22 weeks of age) were processed. One experienced operator (intra-operator analysis) and three different operators (inter-operator) aligned each image to a reference through a rigid registration and selected a volume of interest below the growth plate. From each image the following parameters were measured: total bone mineral content (BMC) and density (BMD), BMC in 40 subregions (ten longitudinal sections, four quadrants), microFE-based stiffness and failure load. Intra-operator reproducibility was acceptable for all parameters (precision error, PE < 3.71%), with lowest reproducibility for stiffness (3.06% at week 18, 3.71% at week 22). The inter-operator reproducibility was slightly lower (PE < 4.25%), although still acceptable for assessing the properties of most interventions. The lowest reproducibility was found for BMC in the lateral sector at the midshaft (PE = 4.25%). Densitometric parameters were more reproducible than most standard morphometric parameters calculated in the proximal trabecular bone. In conclusion, microCT and microFE models provide reproducible measurements for non-invasive assessment of the mouse tibia properties.https://www.frontiersin.org/articles/10.3389/fendo.2022.915938/fullreproducibilitymouse tibiamicroCTmorphometricbone mineralfinite element |
| spellingShingle | Sara Oliviero Sara Oliviero Sara Oliviero Sara Oliviero Vee San Cheong Vee San Cheong Bryant C. Roberts Bryant C. Roberts Carlos Amnael Orozco Diaz William Griffiths Ilaria Bellantuono Ilaria Bellantuono Ilaria Bellantuono Enrico Dall’Ara Enrico Dall’Ara Enrico Dall’Ara Reproducibility of Densitometric and Biomechanical Assessment of the Mouse Tibia From In Vivo Micro-CT Images Frontiers in Endocrinology reproducibility mouse tibia microCT morphometric bone mineral finite element |
| title | Reproducibility of Densitometric and Biomechanical Assessment of the Mouse Tibia From In Vivo Micro-CT Images |
| title_full | Reproducibility of Densitometric and Biomechanical Assessment of the Mouse Tibia From In Vivo Micro-CT Images |
| title_fullStr | Reproducibility of Densitometric and Biomechanical Assessment of the Mouse Tibia From In Vivo Micro-CT Images |
| title_full_unstemmed | Reproducibility of Densitometric and Biomechanical Assessment of the Mouse Tibia From In Vivo Micro-CT Images |
| title_short | Reproducibility of Densitometric and Biomechanical Assessment of the Mouse Tibia From In Vivo Micro-CT Images |
| title_sort | reproducibility of densitometric and biomechanical assessment of the mouse tibia from in vivo micro ct images |
| topic | reproducibility mouse tibia microCT morphometric bone mineral finite element |
| url | https://www.frontiersin.org/articles/10.3389/fendo.2022.915938/full |
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