BuFeiXiaoJiYin ameliorates the NLRP3 inflammation response and gut microbiota in mice with lung cancer companied with Qi-yin deficiency
Abstract Introduction NLRP3 inflammasome responses and gut microbiota have been shown an important role in lung cancer, however, the relationship between gut microbiota and NLRP3 inflammasome responses in lung cancer with Qi-yin deficiency remains elusive. Methods To investigate the effect of the tr...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-03-01
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| Series: | Cancer Cell International |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12935-022-02543-9 |
| _version_ | 1818315569377574912 |
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| author | Rui-yuan Jiang Ting Wang Qiao-yu Lan Yan-chun Qin Ting-ting Man Hua Sun Zhu-long Li Xiao-ting Zhong Chun-mei Mo Zhen Rong |
| author_facet | Rui-yuan Jiang Ting Wang Qiao-yu Lan Yan-chun Qin Ting-ting Man Hua Sun Zhu-long Li Xiao-ting Zhong Chun-mei Mo Zhen Rong |
| author_sort | Rui-yuan Jiang |
| collection | DOAJ |
| description | Abstract Introduction NLRP3 inflammasome responses and gut microbiota have been shown an important role in lung cancer, however, the relationship between gut microbiota and NLRP3 inflammasome responses in lung cancer with Qi-yin deficiency remains elusive. Methods To investigate the effect of the traditional Chinese medicine BuFeiXiaoJiYin (BFXJY) on NLRP3 inflammasome responses and dysbiosis in lung cancer with Qi-yin deficiency, the female BALB/cA-nu mice were treated with LPS and ATP to induce inflammation, and were intragastrically treated with warm Chinese medicine and smoked with shavings to induce Qi-yin deficiency, as well as were injected with 1 × 107/ml A549 cells to simulate lung cancer. Then the three different doses of BuFeiXiaoJiYin (BFXJY) and positive control (CRID3) were used for intervention in mice for 27 consecutive days. Then, we estimated the protection effect of BFXJY on lung cancer mice with Qi-yin deficiency, through deterring tumor growth, NLRP3 inflammasome, PKC signaling, and homeostasis of gut microbiota. Results In this study, we found that BFXJY could inhibit the tumor growth in lung cancer with Qi-yin deficiency by reducing the production of IL-1β and IL-18 and inhibiting NLRP3 inflammasome activation, which might be associated with the inhibition of PKC signaling. Furthermore, BFXJY could promote microbial diversity and balance the microbial composition changes induced by inflammation and Qi-yin deficiency in lung cancer. Conclusion BuFeiXiaoJiYin ameliorates the NLRP3 inflammation response and gut microbiota in mice with lung cancer companied with Qi-yin deficiency. Our study provides a theoretical basis for the clinical development of therapeutic drugs targeting to treat lung cancer. |
| first_indexed | 2024-12-13T09:07:37Z |
| format | Article |
| id | doaj.art-fb12586eceba4c4f8351233c34c07031 |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-12-13T09:07:37Z |
| publishDate | 2022-03-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-fb12586eceba4c4f8351233c34c070312022-12-21T23:53:02ZengBMCCancer Cell International1475-28672022-03-0122111410.1186/s12935-022-02543-9BuFeiXiaoJiYin ameliorates the NLRP3 inflammation response and gut microbiota in mice with lung cancer companied with Qi-yin deficiencyRui-yuan Jiang0Ting Wang1Qiao-yu Lan2Yan-chun Qin3Ting-ting Man4Hua Sun5Zhu-long Li6Xiao-ting Zhong7Chun-mei Mo8Zhen Rong9Department of Graduate Student, Guangxi University of Chinese MedicineDepartment of Dean’s Office, Guangxi University of Chinese MedicineDepartment of Graduate Student, Guangzhou Medical UniversityDepartment of Graduate Student, Guangxi University of Chinese MedicineDepartment of Graduate Student, Guangxi University of Chinese MedicineDepartment of Graduate Student, Guangxi University of Chinese MedicineDepartment of Graduate Student, Guangxi University of Chinese MedicineDepartment of Graduate Student, Guangxi University of Chinese MedicineDepartment of Hepatology, Bao’an Authentic TCM Therapy HospitalDepartment of Oncology, Bao’an Authentic TCM Therapy HospitalAbstract Introduction NLRP3 inflammasome responses and gut microbiota have been shown an important role in lung cancer, however, the relationship between gut microbiota and NLRP3 inflammasome responses in lung cancer with Qi-yin deficiency remains elusive. Methods To investigate the effect of the traditional Chinese medicine BuFeiXiaoJiYin (BFXJY) on NLRP3 inflammasome responses and dysbiosis in lung cancer with Qi-yin deficiency, the female BALB/cA-nu mice were treated with LPS and ATP to induce inflammation, and were intragastrically treated with warm Chinese medicine and smoked with shavings to induce Qi-yin deficiency, as well as were injected with 1 × 107/ml A549 cells to simulate lung cancer. Then the three different doses of BuFeiXiaoJiYin (BFXJY) and positive control (CRID3) were used for intervention in mice for 27 consecutive days. Then, we estimated the protection effect of BFXJY on lung cancer mice with Qi-yin deficiency, through deterring tumor growth, NLRP3 inflammasome, PKC signaling, and homeostasis of gut microbiota. Results In this study, we found that BFXJY could inhibit the tumor growth in lung cancer with Qi-yin deficiency by reducing the production of IL-1β and IL-18 and inhibiting NLRP3 inflammasome activation, which might be associated with the inhibition of PKC signaling. Furthermore, BFXJY could promote microbial diversity and balance the microbial composition changes induced by inflammation and Qi-yin deficiency in lung cancer. Conclusion BuFeiXiaoJiYin ameliorates the NLRP3 inflammation response and gut microbiota in mice with lung cancer companied with Qi-yin deficiency. Our study provides a theoretical basis for the clinical development of therapeutic drugs targeting to treat lung cancer.https://doi.org/10.1186/s12935-022-02543-9Lung cancerQi-yin deficiencyNLRP3 inflammation responseGut microbiotaBuFeiXiaoJiYin |
| spellingShingle | Rui-yuan Jiang Ting Wang Qiao-yu Lan Yan-chun Qin Ting-ting Man Hua Sun Zhu-long Li Xiao-ting Zhong Chun-mei Mo Zhen Rong BuFeiXiaoJiYin ameliorates the NLRP3 inflammation response and gut microbiota in mice with lung cancer companied with Qi-yin deficiency Cancer Cell International Lung cancer Qi-yin deficiency NLRP3 inflammation response Gut microbiota BuFeiXiaoJiYin |
| title | BuFeiXiaoJiYin ameliorates the NLRP3 inflammation response and gut microbiota in mice with lung cancer companied with Qi-yin deficiency |
| title_full | BuFeiXiaoJiYin ameliorates the NLRP3 inflammation response and gut microbiota in mice with lung cancer companied with Qi-yin deficiency |
| title_fullStr | BuFeiXiaoJiYin ameliorates the NLRP3 inflammation response and gut microbiota in mice with lung cancer companied with Qi-yin deficiency |
| title_full_unstemmed | BuFeiXiaoJiYin ameliorates the NLRP3 inflammation response and gut microbiota in mice with lung cancer companied with Qi-yin deficiency |
| title_short | BuFeiXiaoJiYin ameliorates the NLRP3 inflammation response and gut microbiota in mice with lung cancer companied with Qi-yin deficiency |
| title_sort | bufeixiaojiyin ameliorates the nlrp3 inflammation response and gut microbiota in mice with lung cancer companied with qi yin deficiency |
| topic | Lung cancer Qi-yin deficiency NLRP3 inflammation response Gut microbiota BuFeiXiaoJiYin |
| url | https://doi.org/10.1186/s12935-022-02543-9 |
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