Fusarium equiseti as One of the Main Fusarium Species Causing Wilt and Root Rot of Chickpeas in Morocco
Fungal isolates of Fusarium were collected from symptomatic chickpea ( Cicer arietinum L.) plants growing in fields within the Souk Tlat commune in the Gharb region. Morphological and molecular characterizations were performed of the fungal isolate N3 obtained from a chickpea plant. PCR amplifica...
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| Format: | Article |
| Language: | English |
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Polish Botanical Society
2023-09-01
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| Series: | Acta Mycologica |
| Subjects: | |
| Online Access: | https://www.journalssystem.com/amy/-Fusarium-equiseti-as-One-of-the-Main-Fusarium-Species-Causing-Wilt-and-Root-Rot,171904,0,2.html |
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| author | Naila El Hazzat Manal Adnani Soukaina Msairi Moulay Abdelaziz El Alaoui Najoua Mouden Mohamed Chliyeh Said Boughribil Karima Selmaoui Ouazzani Touhami Amina Allal Douira |
| author_facet | Naila El Hazzat Manal Adnani Soukaina Msairi Moulay Abdelaziz El Alaoui Najoua Mouden Mohamed Chliyeh Said Boughribil Karima Selmaoui Ouazzani Touhami Amina Allal Douira |
| author_sort | Naila El Hazzat |
| collection | DOAJ |
| description | Fungal isolates of Fusarium were collected from symptomatic chickpea ( Cicer arietinum L.) plants growing in fields within the Souk Tlat commune in the Gharb region. Morphological and molecular characterizations were performed of the fungal isolate N3 obtained from a chickpea plant. PCR amplification and sequencing of the internal transcribed spacer using the primers ITS1 and ITS4 were applied to identify the fungal isolate N3. The maximum similarity index of the fungus was found to be 99.33% with Fusarium equiseti (accession no. MT111122). In the pathogenicity test, both chickpea seed dip inoculation and soil infestation by the spore suspension of Fusarium isolate were adopted. Four weeks after chickpea seed inoculation, few plants emerged, and those that emerged were stunted. A high percentage of inoculated seeds did not emerge and showed accentuated rot symptoms. Eight weeks after sowing seeds in infested soil, the obtained chickpea seedlings displayed root necrosis, browning at the crown, and wilting. In addition, these plants showed a foliar alteration index of 0.395. The re-isolation was positive for different parts of chickpea plants for both seed and soil inoculation. Fusarium equiseti isolate decreased the length of the root and aerial parts, and the number of leaves and branches of the inoculated chickpea plants either by seed inoculation or soil infestation with values of 0.91 cm and 19.73 cm, 1.29 cm, and 19.44 cm, 1.11 and 18.66, and 0.0 and 2.08 respectively, whereas the corresponding values for the control plants were 27.16 and 28.33 cm, 29.05 and 31.05 cm, 24.21 and 25.66, and 3.50 and 3.11, respectively. To the best of our knowledge, this is the first report of F. equiseti on chickpea ( Cicer arietinum L.) in Morocco. |
| first_indexed | 2024-04-25T00:54:24Z |
| format | Article |
| id | doaj.art-fb126d1d2a014b7d9d3ebf35c4f5a758 |
| institution | Directory Open Access Journal |
| issn | 0001-625X 2353-074X |
| language | English |
| last_indexed | 2024-04-25T00:54:24Z |
| publishDate | 2023-09-01 |
| publisher | Polish Botanical Society |
| record_format | Article |
| series | Acta Mycologica |
| spelling | doaj.art-fb126d1d2a014b7d9d3ebf35c4f5a7582024-03-11T12:58:57ZengPolish Botanical SocietyActa Mycologica0001-625X2353-074X2023-09-015811010.5586/am.576171904Fusarium equiseti as One of the Main Fusarium Species Causing Wilt and Root Rot of Chickpeas in MoroccoNaila El Hazzat0https://orcid.org/0000-0001-7776-0399Manal Adnani1https://orcid.org/0000-0002-8988-5241Soukaina Msairi2https://orcid.org/0000-0002-3632-3077Moulay Abdelaziz El Alaoui3https://orcid.org/0000-0002-1555-5194Najoua Mouden4https://orcid.org/0000-0002-4274-2323Mohamed Chliyeh5https://orcid.org/0000-0003-0454-004XSaid Boughribil6https://orcid.org/0000-0002-2062-8781Karima Selmaoui7https://orcid.org/0000-0002-3374-8280Ouazzani Touhami Amina8https://orcid.org/0000-0001-7373-8645Allal Douira9https://orcid.org/0000-0001-5135-4632Laboratoire des Productions Végétales, Animales et Agro-industrie, Equipe de botanique, Biotechnologie et Protection des Plantes, Département de Biologie, Faculté des Sciences, Université Ibn Tofail, Kénitra, MoroccoLaboratoire des Productions Végétales, Animales et Agro-industrie, Equipe de botanique, Biotechnologie et Protection des Plantes, Département de Biologie, Faculté des Sciences, Université Ibn Tofail, Kénitra, MoroccoLaboratory of Phytobiotechnology, National Agency of Medicinal and Aromatic Plants, Taounate, MoroccoLaboratoire des Productions Végétales, Animales et Agro-industrie, Equipe de botanique, Biotechnologie et Protection des Plantes, Département de Biologie, Faculté des Sciences, Université Ibn Tofail, Kénitra, MoroccoLaboratoire de Chimie Moléculaire et Molécules de l’Environnement, Faculté Pluridisciplinaire de Nador-Université Mohammed 1er Oujda, MoroccoLaboratoire des Productions Végétales, Animales et Agro-industrie, Equipe de botanique, Biotechnologie et Protection des Plantes, Département de Biologie, Faculté des Sciences, Université Ibn Tofail, Kénitra, MoroccoLaboratoire de Virologie Microbiologie, Qualité et Biotechnologie/ Ecotoxicology et Biodiversité, Faculté des Sciences et Techniques – Mohammedia, Hassan II Université de Casablanca, MoroccoLaboratoire des Productions Végétales, Animales et Agro-industrie, Equipe de botanique, Biotechnologie et Protection des Plantes, Département de Biologie, Faculté des Sciences, Université Ibn Tofail, Kénitra, MoroccoLaboratoire des Productions Végétales, Animales et Agro-industrie, Equipe de botanique, Biotechnologie et Protection des Plantes, Département de Biologie, Faculté des Sciences, Université Ibn Tofail, Kénitra, MoroccoLaboratoire des Productions Végétales, Animales et Agro-industrie, Equipe de botanique, Biotechnologie et Protection des Plantes, Département de Biologie, Faculté des Sciences, Université Ibn Tofail, Kénitra, MoroccoFungal isolates of Fusarium were collected from symptomatic chickpea ( Cicer arietinum L.) plants growing in fields within the Souk Tlat commune in the Gharb region. Morphological and molecular characterizations were performed of the fungal isolate N3 obtained from a chickpea plant. PCR amplification and sequencing of the internal transcribed spacer using the primers ITS1 and ITS4 were applied to identify the fungal isolate N3. The maximum similarity index of the fungus was found to be 99.33% with Fusarium equiseti (accession no. MT111122). In the pathogenicity test, both chickpea seed dip inoculation and soil infestation by the spore suspension of Fusarium isolate were adopted. Four weeks after chickpea seed inoculation, few plants emerged, and those that emerged were stunted. A high percentage of inoculated seeds did not emerge and showed accentuated rot symptoms. Eight weeks after sowing seeds in infested soil, the obtained chickpea seedlings displayed root necrosis, browning at the crown, and wilting. In addition, these plants showed a foliar alteration index of 0.395. The re-isolation was positive for different parts of chickpea plants for both seed and soil inoculation. Fusarium equiseti isolate decreased the length of the root and aerial parts, and the number of leaves and branches of the inoculated chickpea plants either by seed inoculation or soil infestation with values of 0.91 cm and 19.73 cm, 1.29 cm, and 19.44 cm, 1.11 and 18.66, and 0.0 and 2.08 respectively, whereas the corresponding values for the control plants were 27.16 and 28.33 cm, 29.05 and 31.05 cm, 24.21 and 25.66, and 3.50 and 3.11, respectively. To the best of our knowledge, this is the first report of F. equiseti on chickpea ( Cicer arietinum L.) in Morocco.https://www.journalssystem.com/amy/-Fusarium-equiseti-as-One-of-the-Main-Fusarium-Species-Causing-Wilt-and-Root-Rot,171904,0,2.htmlchickpeasinoculationfusarium equisetirotwilting |
| spellingShingle | Naila El Hazzat Manal Adnani Soukaina Msairi Moulay Abdelaziz El Alaoui Najoua Mouden Mohamed Chliyeh Said Boughribil Karima Selmaoui Ouazzani Touhami Amina Allal Douira Fusarium equiseti as One of the Main Fusarium Species Causing Wilt and Root Rot of Chickpeas in Morocco Acta Mycologica chickpeas inoculation fusarium equiseti rot wilting |
| title | Fusarium equiseti as One of the Main Fusarium Species Causing Wilt and Root Rot of Chickpeas in Morocco |
| title_full | Fusarium equiseti as One of the Main Fusarium Species Causing Wilt and Root Rot of Chickpeas in Morocco |
| title_fullStr | Fusarium equiseti as One of the Main Fusarium Species Causing Wilt and Root Rot of Chickpeas in Morocco |
| title_full_unstemmed | Fusarium equiseti as One of the Main Fusarium Species Causing Wilt and Root Rot of Chickpeas in Morocco |
| title_short | Fusarium equiseti as One of the Main Fusarium Species Causing Wilt and Root Rot of Chickpeas in Morocco |
| title_sort | fusarium equiseti as one of the main fusarium species causing wilt and root rot of chickpeas in morocco |
| topic | chickpeas inoculation fusarium equiseti rot wilting |
| url | https://www.journalssystem.com/amy/-Fusarium-equiseti-as-One-of-the-Main-Fusarium-Species-Causing-Wilt-and-Root-Rot,171904,0,2.html |
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