Comparison of Genomic Polymorphisms and Genetic Relation of Clinical Salmonella Enterica Serovar Typhimurium Isolates in Kerman Province by ERIC- PCR and Box-PCR Methods
Introduction: Salmonella is one of the most important causes of gastroenteritis in humans. Salmonella enterica Serovar Typhimurium has many hosts in addition to humans, and its prevalence in the community is high. The aim of the study was comparing the genetic diversity of Salmonella enterica serova...
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| Format: | Article |
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Shahid Sadoughi University of Medical Sciences
2017-10-01
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| Series: | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd |
| Subjects: | |
| Online Access: | http://jssu.ssu.ac.ir/browse.php?a_code=A-10-2801-1&slc_lang=en&sid=1 |
| _version_ | 1818190167759912960 |
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| author | Arghavan Geranmayeh Farzaneh Hoseini Robab Rafiei Tabatabaei |
| author_facet | Arghavan Geranmayeh Farzaneh Hoseini Robab Rafiei Tabatabaei |
| author_sort | Arghavan Geranmayeh |
| collection | DOAJ |
| description | Introduction: Salmonella is one of the most important causes of gastroenteritis in humans. Salmonella enterica Serovar Typhimurium has many hosts in addition to humans, and its prevalence in the community is high. The aim of the study was comparing the genetic diversity of Salmonella enterica serovar Typhimurium isolated from human fecal samples by both of ERIC-PCR and BOX-PCR method.
Methods: In this cross-sectional study, 60 Salmonella enterica serovar Typhimurium were obtained from the human fecal samples. These strains were identified by standard microbiological and biochemical tests. Then, ERIC-PCR and BOX-PCR were carried out for determination of molecular relatedness of Salmonella enterica serovar Typhimurium using specific primers.
Results: The results showed that all 60 Salmonella enterica serovar Typhimurium were separable using ERIC 1, ERIC 2 and BOXAIR. In electrophoresis, 2-11 bands with 20-3200bp for ERIC-PCR and 2-10 bands with 200-1500bp for BOX-PCR were observed. Therefore, 13 different clusters (C1-C13) in ERIC-PCR and 21 different clusters (C1-C21) in BOX-PCR were identified.
Conclusion: The results showed that Salmonella enterica serovar Typhimurium strains were non-homolog. Therefore, ERIC-PCR and BOX-PCR methods are appropriate methods for molecular typing of Salmonella strains and determine the original infection source for the epidemiological survey as well as infection prevention program. |
| first_indexed | 2024-12-11T23:54:25Z |
| format | Article |
| id | doaj.art-fb149eadf461467293c0e60da3a8bfed |
| institution | Directory Open Access Journal |
| issn | 2228-5741 2228-5733 |
| language | fas |
| last_indexed | 2024-12-11T23:54:25Z |
| publishDate | 2017-10-01 |
| publisher | Shahid Sadoughi University of Medical Sciences |
| record_format | Article |
| series | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd |
| spelling | doaj.art-fb149eadf461467293c0e60da3a8bfed2022-12-22T00:45:23ZfasShahid Sadoughi University of Medical SciencesMajallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd2228-57412228-57332017-10-01257564571Comparison of Genomic Polymorphisms and Genetic Relation of Clinical Salmonella Enterica Serovar Typhimurium Isolates in Kerman Province by ERIC- PCR and Box-PCR MethodsArghavan Geranmayeh0Farzaneh Hoseini1Robab Rafiei Tabatabaei2 Department of Microbiology, College of Bioscience, Islamic Azad University Tehran North Branch, Tehran, Iran. Department of Microbiology, College of Bioscience, Islamic Azad University Tehran North Branch, Tehran, Iran. Department of Microbiology, College of Bioscience, Islamic Azad University Tehran North Branch, Tehran, Iran. Introduction: Salmonella is one of the most important causes of gastroenteritis in humans. Salmonella enterica Serovar Typhimurium has many hosts in addition to humans, and its prevalence in the community is high. The aim of the study was comparing the genetic diversity of Salmonella enterica serovar Typhimurium isolated from human fecal samples by both of ERIC-PCR and BOX-PCR method. Methods: In this cross-sectional study, 60 Salmonella enterica serovar Typhimurium were obtained from the human fecal samples. These strains were identified by standard microbiological and biochemical tests. Then, ERIC-PCR and BOX-PCR were carried out for determination of molecular relatedness of Salmonella enterica serovar Typhimurium using specific primers. Results: The results showed that all 60 Salmonella enterica serovar Typhimurium were separable using ERIC 1, ERIC 2 and BOXAIR. In electrophoresis, 2-11 bands with 20-3200bp for ERIC-PCR and 2-10 bands with 200-1500bp for BOX-PCR were observed. Therefore, 13 different clusters (C1-C13) in ERIC-PCR and 21 different clusters (C1-C21) in BOX-PCR were identified. Conclusion: The results showed that Salmonella enterica serovar Typhimurium strains were non-homolog. Therefore, ERIC-PCR and BOX-PCR methods are appropriate methods for molecular typing of Salmonella strains and determine the original infection source for the epidemiological survey as well as infection prevention program.http://jssu.ssu.ac.ir/browse.php?a_code=A-10-2801-1&slc_lang=en&sid=1Salmonella enterica serovar Typhimurium BOX-PCR ERIC-PCR. |
| spellingShingle | Arghavan Geranmayeh Farzaneh Hoseini Robab Rafiei Tabatabaei Comparison of Genomic Polymorphisms and Genetic Relation of Clinical Salmonella Enterica Serovar Typhimurium Isolates in Kerman Province by ERIC- PCR and Box-PCR Methods Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd Salmonella enterica serovar Typhimurium BOX-PCR ERIC-PCR. |
| title | Comparison of Genomic Polymorphisms and Genetic Relation of Clinical Salmonella Enterica Serovar Typhimurium Isolates in Kerman Province by ERIC- PCR and Box-PCR Methods |
| title_full | Comparison of Genomic Polymorphisms and Genetic Relation of Clinical Salmonella Enterica Serovar Typhimurium Isolates in Kerman Province by ERIC- PCR and Box-PCR Methods |
| title_fullStr | Comparison of Genomic Polymorphisms and Genetic Relation of Clinical Salmonella Enterica Serovar Typhimurium Isolates in Kerman Province by ERIC- PCR and Box-PCR Methods |
| title_full_unstemmed | Comparison of Genomic Polymorphisms and Genetic Relation of Clinical Salmonella Enterica Serovar Typhimurium Isolates in Kerman Province by ERIC- PCR and Box-PCR Methods |
| title_short | Comparison of Genomic Polymorphisms and Genetic Relation of Clinical Salmonella Enterica Serovar Typhimurium Isolates in Kerman Province by ERIC- PCR and Box-PCR Methods |
| title_sort | comparison of genomic polymorphisms and genetic relation of clinical salmonella enterica serovar typhimurium isolates in kerman province by eric pcr and box pcr methods |
| topic | Salmonella enterica serovar Typhimurium BOX-PCR ERIC-PCR. |
| url | http://jssu.ssu.ac.ir/browse.php?a_code=A-10-2801-1&slc_lang=en&sid=1 |
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