The Fibrolytic Enzyme Profiles and the Composition of Fungal Communities in Donkey Cecum-Colon Ecosystem
The fibrolytic enzymes and the hindgut fungi in donkey cecum-colon ecosystem play an important role in dietary fiber digestion. A better understanding of the fibrolytic enzyme profiles and the fungal community along donkey caecum and colon is key for optimizing hindgut function. In the present study...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-02-01
|
| Series: | Animals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2615/12/4/412 |
| _version_ | 1797483572972486656 |
|---|---|
| author | Zhenwei Zhang Yonghui Wang Bingjian Huang Mingxia Zhu Changfa Wang |
| author_facet | Zhenwei Zhang Yonghui Wang Bingjian Huang Mingxia Zhu Changfa Wang |
| author_sort | Zhenwei Zhang |
| collection | DOAJ |
| description | The fibrolytic enzymes and the hindgut fungi in donkey cecum-colon ecosystem play an important role in dietary fiber digestion. A better understanding of the fibrolytic enzyme profiles and the fungal community along donkey caecum and colon is key for optimizing hindgut function. In the present study, the fibrolytic enzyme activities within donkey caecum and colon were firstly measured by spectrophotometry. Activities of carboxymethyl cellulase, avicelase, xylanase, and acetyl esterase were greater in donkey dorsal colon than in caecum, indicating that the colon microorganisms may be more efficient in producing fibrolytic enzymes compared to caecum microbes. The fungal community composition along donkey hindgut was determined by sequencing ITS region using Illumina MiSeq. Three fungal phyla were identified by sequence comparison: <i>Ascomycota</i> (66.8%–74.4%), <i>Basidiomycota</i> (21.6%–30.9%), and <i>Neocallimastigomycota</i> (0.9%–3.3%). The <i>Aspergillus</i>, <i>Wallemia</i>, <i>Phanerochaete</i>, <i>Fusarium</i>, and <i>Penicillium</i> were detected as the dominant genera, but their metabolic and functional significance in donkey cecum-colon ecosystem need further investigation. In terms of the anaerobic fungi <i>Neocallimastigomycota</i>, its abundance was greater in donkey colon than in caecum (<i>p</i> < 0.05), indicating that the donkey hindgut region was associated with differences in fungal community composition. Moreover, the relative abundance of enzymes related to plant cell wall degradation were predicted by PICRUSt, and they were also lower in caecum than in colon. The present study provided new information about fibrolytic enzyme profiles and fungal composition in donkey hindgut ecosystem. |
| first_indexed | 2024-03-09T22:48:57Z |
| format | Article |
| id | doaj.art-fb18ccd2eb34439091618af44bfaa8ed |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T22:48:57Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-fb18ccd2eb34439091618af44bfaa8ed2023-11-23T18:24:40ZengMDPI AGAnimals2076-26152022-02-0112441210.3390/ani12040412The Fibrolytic Enzyme Profiles and the Composition of Fungal Communities in Donkey Cecum-Colon EcosystemZhenwei Zhang0Yonghui Wang1Bingjian Huang2Mingxia Zhu3Changfa Wang4Liaocheng Research Institute of Donkey High-Efficiency Breeding and Ecological Feeding, Liaocheng University, Liaocheng 252059, ChinaLiaocheng Research Institute of Donkey High-Efficiency Breeding and Ecological Feeding, Liaocheng University, Liaocheng 252059, ChinaLiaocheng Research Institute of Donkey High-Efficiency Breeding and Ecological Feeding, Liaocheng University, Liaocheng 252059, ChinaLiaocheng Research Institute of Donkey High-Efficiency Breeding and Ecological Feeding, Liaocheng University, Liaocheng 252059, ChinaLiaocheng Research Institute of Donkey High-Efficiency Breeding and Ecological Feeding, Liaocheng University, Liaocheng 252059, ChinaThe fibrolytic enzymes and the hindgut fungi in donkey cecum-colon ecosystem play an important role in dietary fiber digestion. A better understanding of the fibrolytic enzyme profiles and the fungal community along donkey caecum and colon is key for optimizing hindgut function. In the present study, the fibrolytic enzyme activities within donkey caecum and colon were firstly measured by spectrophotometry. Activities of carboxymethyl cellulase, avicelase, xylanase, and acetyl esterase were greater in donkey dorsal colon than in caecum, indicating that the colon microorganisms may be more efficient in producing fibrolytic enzymes compared to caecum microbes. The fungal community composition along donkey hindgut was determined by sequencing ITS region using Illumina MiSeq. Three fungal phyla were identified by sequence comparison: <i>Ascomycota</i> (66.8%–74.4%), <i>Basidiomycota</i> (21.6%–30.9%), and <i>Neocallimastigomycota</i> (0.9%–3.3%). The <i>Aspergillus</i>, <i>Wallemia</i>, <i>Phanerochaete</i>, <i>Fusarium</i>, and <i>Penicillium</i> were detected as the dominant genera, but their metabolic and functional significance in donkey cecum-colon ecosystem need further investigation. In terms of the anaerobic fungi <i>Neocallimastigomycota</i>, its abundance was greater in donkey colon than in caecum (<i>p</i> < 0.05), indicating that the donkey hindgut region was associated with differences in fungal community composition. Moreover, the relative abundance of enzymes related to plant cell wall degradation were predicted by PICRUSt, and they were also lower in caecum than in colon. The present study provided new information about fibrolytic enzyme profiles and fungal composition in donkey hindgut ecosystem.https://www.mdpi.com/2076-2615/12/4/412donkeycaecumcolonfibrolytic enzymefungal composition |
| spellingShingle | Zhenwei Zhang Yonghui Wang Bingjian Huang Mingxia Zhu Changfa Wang The Fibrolytic Enzyme Profiles and the Composition of Fungal Communities in Donkey Cecum-Colon Ecosystem Animals donkey caecum colon fibrolytic enzyme fungal composition |
| title | The Fibrolytic Enzyme Profiles and the Composition of Fungal Communities in Donkey Cecum-Colon Ecosystem |
| title_full | The Fibrolytic Enzyme Profiles and the Composition of Fungal Communities in Donkey Cecum-Colon Ecosystem |
| title_fullStr | The Fibrolytic Enzyme Profiles and the Composition of Fungal Communities in Donkey Cecum-Colon Ecosystem |
| title_full_unstemmed | The Fibrolytic Enzyme Profiles and the Composition of Fungal Communities in Donkey Cecum-Colon Ecosystem |
| title_short | The Fibrolytic Enzyme Profiles and the Composition of Fungal Communities in Donkey Cecum-Colon Ecosystem |
| title_sort | fibrolytic enzyme profiles and the composition of fungal communities in donkey cecum colon ecosystem |
| topic | donkey caecum colon fibrolytic enzyme fungal composition |
| url | https://www.mdpi.com/2076-2615/12/4/412 |
| work_keys_str_mv | AT zhenweizhang thefibrolyticenzymeprofilesandthecompositionoffungalcommunitiesindonkeycecumcolonecosystem AT yonghuiwang thefibrolyticenzymeprofilesandthecompositionoffungalcommunitiesindonkeycecumcolonecosystem AT bingjianhuang thefibrolyticenzymeprofilesandthecompositionoffungalcommunitiesindonkeycecumcolonecosystem AT mingxiazhu thefibrolyticenzymeprofilesandthecompositionoffungalcommunitiesindonkeycecumcolonecosystem AT changfawang thefibrolyticenzymeprofilesandthecompositionoffungalcommunitiesindonkeycecumcolonecosystem AT zhenweizhang fibrolyticenzymeprofilesandthecompositionoffungalcommunitiesindonkeycecumcolonecosystem AT yonghuiwang fibrolyticenzymeprofilesandthecompositionoffungalcommunitiesindonkeycecumcolonecosystem AT bingjianhuang fibrolyticenzymeprofilesandthecompositionoffungalcommunitiesindonkeycecumcolonecosystem AT mingxiazhu fibrolyticenzymeprofilesandthecompositionoffungalcommunitiesindonkeycecumcolonecosystem AT changfawang fibrolyticenzymeprofilesandthecompositionoffungalcommunitiesindonkeycecumcolonecosystem |