Liquid-Crystalline Dispersions of Double-Stranded DNA
In this review, we compare the circular dichroism (CD) spectra of liquid-crystalline dispersion (LCD) particles formed in PEG-containing aqueous-salt solutions with the purpose of determining the packing of ds DNA molecules in these particles. Depending on the osmotic pressure of the solution, the p...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2019-03-01
|
| Series: | Crystals |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4352/9/3/162 |
| _version_ | 1798006424974917632 |
|---|---|
| author | Yuri Yevdokimov Sergey Skuridin Viktor Salyanov Sergey Semenov Efim Kats |
| author_facet | Yuri Yevdokimov Sergey Skuridin Viktor Salyanov Sergey Semenov Efim Kats |
| author_sort | Yuri Yevdokimov |
| collection | DOAJ |
| description | In this review, we compare the circular dichroism (CD) spectra of liquid-crystalline dispersion (LCD) particles formed in PEG-containing aqueous-salt solutions with the purpose of determining the packing of ds DNA molecules in these particles. Depending on the osmotic pressure of the solution, the phase exclusion of ds DNA molecules at room temperature results in the formation of LCD particles with the cholesteric or the hexagonal packing of molecules. The heating of dispersion particles with the hexagonal packing of the ds DNA molecules results in a new phase transition, accompanied by an appearance of a new optically active phase of ds DNA molecules. Our results are rationalized by way of a concept of orientationally ordered “quasinematic„ layers formed by ds DNA molecules, with a parallel alignment in the hexagonal structure. These layers can adopt a twisted configuration with a temperature increase; and as a result of this process, a new, helicoidal structure of dispersion particle is formed (termed as the “re-entrant„ cholesteric phase). To prove the cholesteric pattern of ds DNA molecules in this phase, the “liquid-like„ state of the dispersion particles was transformed into its “rigid„ counterpart. |
| first_indexed | 2024-04-11T12:54:34Z |
| format | Article |
| id | doaj.art-fb1ed8bbd91a457682033938e290eb5c |
| institution | Directory Open Access Journal |
| issn | 2073-4352 |
| language | English |
| last_indexed | 2024-04-11T12:54:34Z |
| publishDate | 2019-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Crystals |
| spelling | doaj.art-fb1ed8bbd91a457682033938e290eb5c2022-12-22T04:23:07ZengMDPI AGCrystals2073-43522019-03-019316210.3390/cryst9030162cryst9030162Liquid-Crystalline Dispersions of Double-Stranded DNAYuri Yevdokimov0Sergey Skuridin1Viktor Salyanov2Sergey Semenov3Efim Kats4Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences, Vavilova, St. 32, Moscow 119991, RussiaEngelhardt Institute of Molecular Biology of the Russian Academy of Sciences, Vavilova, St. 32, Moscow 119991, RussiaEngelhardt Institute of Molecular Biology of the Russian Academy of Sciences, Vavilova, St. 32, Moscow 119991, RussiaNational Research Centre “Kurchatov Institute”, Kurchatova Sq. 1, Moscow 123182, RussiaLandau Institute for Theoretical Physics of the Russian Academy of Sciences, Academician Semenov Ave. 1a, Chernogolovka, Moscow Region 142432, RussiaIn this review, we compare the circular dichroism (CD) spectra of liquid-crystalline dispersion (LCD) particles formed in PEG-containing aqueous-salt solutions with the purpose of determining the packing of ds DNA molecules in these particles. Depending on the osmotic pressure of the solution, the phase exclusion of ds DNA molecules at room temperature results in the formation of LCD particles with the cholesteric or the hexagonal packing of molecules. The heating of dispersion particles with the hexagonal packing of the ds DNA molecules results in a new phase transition, accompanied by an appearance of a new optically active phase of ds DNA molecules. Our results are rationalized by way of a concept of orientationally ordered “quasinematic„ layers formed by ds DNA molecules, with a parallel alignment in the hexagonal structure. These layers can adopt a twisted configuration with a temperature increase; and as a result of this process, a new, helicoidal structure of dispersion particle is formed (termed as the “re-entrant„ cholesteric phase). To prove the cholesteric pattern of ds DNA molecules in this phase, the “liquid-like„ state of the dispersion particles was transformed into its “rigid„ counterpart.https://www.mdpi.com/2073-4352/9/3/162liquid-crystalline dispersions of DNAcholesteric and hexagonal packing of DNAtheoretically calculated and experimental circular dichroism spectratextures of the DNA liquid-crystalline phases“re-entrant” cholesteric phase of DNAanthracyclines drugschelate complexesnanobridges“rigid” particles of DNA |
| spellingShingle | Yuri Yevdokimov Sergey Skuridin Viktor Salyanov Sergey Semenov Efim Kats Liquid-Crystalline Dispersions of Double-Stranded DNA Crystals liquid-crystalline dispersions of DNA cholesteric and hexagonal packing of DNA theoretically calculated and experimental circular dichroism spectra textures of the DNA liquid-crystalline phases “re-entrant” cholesteric phase of DNA anthracyclines drugs chelate complexes nanobridges “rigid” particles of DNA |
| title | Liquid-Crystalline Dispersions of Double-Stranded DNA |
| title_full | Liquid-Crystalline Dispersions of Double-Stranded DNA |
| title_fullStr | Liquid-Crystalline Dispersions of Double-Stranded DNA |
| title_full_unstemmed | Liquid-Crystalline Dispersions of Double-Stranded DNA |
| title_short | Liquid-Crystalline Dispersions of Double-Stranded DNA |
| title_sort | liquid crystalline dispersions of double stranded dna |
| topic | liquid-crystalline dispersions of DNA cholesteric and hexagonal packing of DNA theoretically calculated and experimental circular dichroism spectra textures of the DNA liquid-crystalline phases “re-entrant” cholesteric phase of DNA anthracyclines drugs chelate complexes nanobridges “rigid” particles of DNA |
| url | https://www.mdpi.com/2073-4352/9/3/162 |
| work_keys_str_mv | AT yuriyevdokimov liquidcrystallinedispersionsofdoublestrandeddna AT sergeyskuridin liquidcrystallinedispersionsofdoublestrandeddna AT viktorsalyanov liquidcrystallinedispersionsofdoublestrandeddna AT sergeysemenov liquidcrystallinedispersionsofdoublestrandeddna AT efimkats liquidcrystallinedispersionsofdoublestrandeddna |